Phenotypic characterization of pectinase-producing alkaliphilic microbial isolates from Lake Bogoria, Baringo County, Kenya

Phenotypic characterization of pectinase-producing alkaliphilic microbial isolates from Lake Bogoria, Baringo County, Kenya | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL MicrobiologyOpen This is a preprint and has not been peer reviewed. Data may be preliminary. 17 February 2025 V1 Latest version Share on Phenotypic characterization of pectinase-producing alkaliphilic microbial isolates from Lake Bogoria, Baringo County, Kenya Authors : Kevin Raymond Oluoch 0000-0001-5876-3754 [email protected] , Edward Kirwa Muge 0000-0001-6332-6874 , Maxwell Omondi Onyango , and Francis Jakim Mulaa Authors Info & Affiliations https://doi.org/10.22541/au.173980280.07061246/v1 Published MicrobiologyOpen Version of record Peer review timeline 419 views 171 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Alkaline pectinases are in demand in industrial processes that require the degradation of plant pectins at high pH, e.g., removal of pectin stains from fabrics, cutlery, and porcelain; treatment of pectic wastewater; fermentation of coffee, tea, and cocoa; manufacture of poultry and animal feeds, and processing of textiles, etc. The present study aimed to a) screen four alkaliphilic microbial isolates, previously obtained from samples collected around Lake Bogoria (soda lake), Baringo County, Kenya, for alkaline pectinases, and b) characterize the pectinase-producers. The screening data revealed that all the isolates were pectinase producers, exhibiting catalytic activities that ranged from 1.4 - 1.6 pectinolytic indices (PI) (primary screening) and 0.04 - 0.15 U/ml (secondary screening). These isolates’ colonies featured smooth textures and umbonate elevations and were moist, white, or cream. Their cells were motile, aerobic rods that were Gram-, catalase-, and oxidase-positive. They utilized inositol, sucrose, lactose, and glucose and hydrolyzed starch, pullulan, casein, and gelatin. They grew optimally at pH 10.5, 45 °C, and without NaCl but tolerated growth at higher temperatures (up to 55 °C) and saline conditions [up to 12.5 % (w/v) NaCl]. At neutral pH, no growth was detected. Based on these phenotypic characteristics, the Lake Bogoria alkaliphilic microbial isolates were identified as Bacillus halodurans , capable of producing alkaline pectinases that, if harnessed, can potentially find applications in the aforementioned industrial processes. Supplementary Material File (manuscript - microbiologyopen.docx) Download 1.20 MB Information & Authors Information Version history V1 Version 1 17 February 2025 Peer review timeline Published MicrobiologyOpen Version of Record 9 Sep 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection MicrobiologyOpen Keywords bacillus biodegradation secondary metabolites Authors Affiliations Kevin Raymond Oluoch 0000-0001-5876-3754 [email protected] University of Nairobi Department of Biochemistry View all articles by this author Edward Kirwa Muge 0000-0001-6332-6874 University of Nairobi Department of Biochemistry View all articles by this author Maxwell Omondi Onyango University of Nairobi Faculty of Veterinary Medicine View all articles by this author Francis Jakim Mulaa University of Nairobi Department of Biochemistry View all articles by this author Metrics & Citations Metrics Article Usage 419 views 171 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Kevin Raymond Oluoch, Edward Kirwa Muge, Maxwell Omondi Onyango, et al. Phenotypic characterization of pectinase-producing alkaliphilic microbial isolates from Lake Bogoria, Baringo County, Kenya. Authorea . 17 February 2025. DOI: https://doi.org/10.22541/au.173980280.07061246/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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