MiR22-5p, TET2, and 5-hmC expression in endometrium of minimal/mild endometriosis and normal endometrium.

(A) RT-qPCR analysis of miR22-5p and (B) TET2 expression in endometrium of minimal/mild endometriosis (n = 13) and normal endometrium (n = 11). miRNA expression was normalized to U6 snRNA expression, and mRNA expression was normalized to GAPDH expression. (C) Western blot analysis of TET2 expression in mild endometriosis (n = 6) and in normal endometrium (n = 5), normalized to GAPDH expression. (D) Representative Immunohistochemistry images showing TET2 and 5-hmC in the cytoplasmic and nuclear regions of stromal and epithelial cells in endometrium of mild endometriosis and normal endometrium (n = 3). Black arrows indicate positive staining. Scale bars, 200 μm. Data represent the mean ± SD and were analyzed by Student’s t-test.