MiR22-5p, TET2, and 5-hmC expression in endometrium of minimal/mild endometriosis and normal endometrium.

Li Xiao (307908), Tianjiao Pei (9115734), Wei Huang (36889), Min Zhou (173135), Jing Fu (177892), Jing Tan (2230783), Tingting Liu (267387), Yong Song (286107), Shiyuan Yang (9115737)
2020 · doi:10.1371/journal.pone.0234086.g001 · W6923157491
other OA: green CC0
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⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-07-05 ⓘ

This study quantified miR22-5p, TET2 mRNA and protein, and 5-hmC expression in endometrial tissues from women with minimal/mild endometriosis and healthy controls.

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The study evaluated whether miR-22-5p, TET2, and 5-hmC are differentially expressed in endometrium during the implantation window in women with minimal/mild endometriosis compared with normal endometrium. Using RT-qPCR, Western blot, and immunohistochemistry, the authors measured miR-22-5p and TET2 expression and assessed 5-hmC staining patterns in endometrial stromal and epithelial cells, finding changes associated with minimal/mild endometriosis (with expression normalized to U6 and GAPDH, respectively). The analysis relied on small sample sizes for some assays (e.g., Western blot and immunohistochemistry) and compared limited groups (n=13 minimal/mild endometriosis vs n=11 normal for RT-qPCR). This paper is centrally about endometriosis — it specifically analyzes miR22-5p, TET2, and 5-hmC expression in endometrium of minimal/mild endometriosis during the implantation window.

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Abstract

(A) RT-qPCR analysis of miR22-5p and (B) TET2 expression in endometrium of minimal/mild endometriosis (n = 13) and normal endometrium (n = 11). miRNA expression was normalized to U6 snRNA expression, and mRNA expression was normalized to GAPDH expression. (C) Western blot analysis of TET2 expression in mild endometriosis (n = 6) and in normal endometrium (n = 5), normalized to GAPDH expression. (D) Representative Immunohistochemistry images showing TET2 and 5-hmC in the cytoplasmic and nuclear regions of stromal and epithelial cells in endometrium of mild endometriosis and normal endometrium (n = 3). Black arrows indicate positive staining. Scale bars, 200 μm. Data represent the mean ± SD and were analyzed by Student’s t-test.
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MicroRNA22-5p targets ten-eleven translocation and regulates estrogen receptor 2 expression in infertile women with minimal/mild endometriosis during implantation window Fig 1 MiR22-5p, TET2, and 5-hmC expression in endometrium of minimal/mild endometriosis and normal endometrium. (A) RT-qPCR analysis of miR22-5p and (B) TET2 expression in endometrium of minimal/mild endometriosis (n = 13) and normal endometrium (n = 11). miRNA expression was normalized to U6 snRNA expression, and mRNA expression was normalized to GAPDH expression. (C) Western blot analysis of TET2 expression in mild endometriosis (n = 6) and in normal endometrium (n = 5), normalized to GAPDH expression. (D) Representative Immunohistochemistry images showing TET2 and 5-hmC in the cytoplasmic and nuclear regions of stromal and epithelial cells in endometrium of mild endometriosis and normal endometrium (n = 3). Black arrows indicate positive staining. Scale bars, 200 μm. Data represent the mean ± SD and were analyzed by Student’s t-test.

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endometriosis

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