Priming of ash saplings with a low virulent Hymenoscyphus fraxineus strain as a possible disease control approach for reducing symptoms of ash dieback

Priming of ash saplings with a low virulent Hymenoscyphus fraxineus strain as a possible disease control approach for reducing symptoms of ash dieback | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Priming of ash saplings with a low virulent Hymenoscyphus fraxineus strain as a possible disease control approach for reducing symptoms of ash dieback Maia Ridley, Özge Demir, Esteban Charria-Girón, Barbara Schulz, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4348277/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 31 Jan, 2025 Read the published version in Journal of Plant Diseases and Protection → Version 1 posted 4 You are reading this latest preprint version Abstract Ash dieback is a tree disease caused by the fungal pathogen Hymenoscyphus fraxineus. Since its introduction into Europe, it has caused widespread and significant losses of the European ash, Fraxinus excelsior. Inoculations of F. excelsior with a low virulent H. fraxineus isolate was assessed as a promising method for reducing symptoms associated with ash dieback, presumably by triggering systemic induced resistance. Two strains of H. fraxineus were chosen based on observations of high and low in planta virulence. Crude extracts obtained from cultures of the highly virulent strain were more phytotoxic in a leaf puncture assay than ones obtained from the low virulent strain. UHPLC-DAD-MS/MS data identified the phytotoxin viridiol and the potential phytotoxin hyfraxin A in both cultures. However, the production of these compounds in vitro did not correspond with virulence in planta. To test the effects of priming, saplings of F. excelsior were first inoculated with the low virulent strain and subsequently with the highly virulent strain. On average, necrosis expansion on the stems was reduced by 54% in primed saplings at the end of the monitoring period of 14 weeks, thus providing proof of concept for priming. These results contribute to our understanding of a possible integrated biological disease control approach for increasing resistance in saplings and reducing potential damages associated with pathogens, particularly during nursery propagation, out-planting and through the establishment phase. We discuss the results in the context of relevant literature and summarise the limited availability of literature on priming and underlying principles in trees. ash dieback biocontrol Fraxinus excelsior induced resistance priming secondary metabolites Full Text Cite Share Download PDF Status: Published Journal Publication published 31 Jan, 2025 Read the published version in Journal of Plant Diseases and Protection → Version 1 posted Reviewers invited by journal 03 May, 2024 Editor invited by journal 03 May, 2024 Editor assigned by journal 02 May, 2024 First submitted to journal 30 Apr, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4348277","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":298274356,"identity":"be46a077-871e-48f1-aef1-7939df7be978","order_by":0,"name":"Maia 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Since its introduction into Europe, it has caused widespread and significant losses of the European ash, Fraxinus excelsior. Inoculations of F. excelsior with a low virulent H. fraxineus isolate was assessed as a promising method for reducing symptoms associated with ash dieback, presumably by triggering systemic induced resistance. Two strains of H. fraxineus were chosen based on observations of high and low in planta virulence. Crude extracts obtained from cultures of the highly virulent strain were more phytotoxic in a leaf puncture assay than ones obtained from the low virulent strain. UHPLC-DAD-MS/MS data identified the phytotoxin viridiol and the potential phytotoxin hyfraxin A in both cultures. However, the production of these compounds in vitro did not correspond with virulence in planta.\nTo test the effects of priming, saplings of F. excelsior were first inoculated with the low virulent strain and subsequently with the highly virulent strain. On average, necrosis expansion on the stems was reduced by 54% in primed saplings at the end of the monitoring period of 14 weeks, thus providing proof of concept for priming. These results contribute to our understanding of a possible integrated biological disease control approach for increasing resistance in saplings and reducing potential damages associated with pathogens, particularly during nursery propagation, out-planting and through the establishment phase. 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