Was fishing village of Lepenski Vir built by Europe’s first farmers?

Today, it is widely accepted that agriculture and settled village life arrived in Europe as a cultural package, carried by people migrating from Anatolia and the Aegean Basin. The putative fisher-forager site of Lepenski Vir in Serbia has long been acknowledged as an exception to this model. Here, the Mesolithic-Neolithic transition - possibly inspired by interaction with the new arrivals - was thought to have taken place autochthonously on site. Our reinterpretation, based on ancient genomes, as well as archaeological and isotopic evidence, revisits this conclusion, indicating that here too, house construction, early village society and agriculture were primarily associated with Europe’s first farmers, thus challenging the long-held interpretation of Lepenski Vir as a Mesolithic community that adopted Neolithic practices. A detailed timeline of population changes at the site suggests that Aegean incomers did not simply integrate into an established Mesolithic society, rather founded new lineages and households. Iron Gates foragers and their admixed descendants appear to have been buried largely separately, on the fringes of the settlement, their diet showing no major shift from aquatic to terrestrial food resources.