Copper Nanoparticles by Rhodotorula diobovata as an antimicrobial agent against Antibiotic Resistance

Copper Nanoparticles by Rhodotorula diobovata as an antimicrobial agent against Antibiotic Resistance | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Copper Nanoparticles by Rhodotorula diobovata as an antimicrobial agent against Antibiotic Resistance Batool M. Ubed, Enas A. Bady, Ahmed A. A. Alsaad, Rafeef Amer Abdul-Jabar, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6738954/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Copper's distinctive physical and chemical characteristics render it a prevalent metallic element. This research aimed to explore the eco-friendly, straightforward, and cost-effective green synthesis of copper nanoparticles (Cu-NPs) using Rhodotorula diobovata Strain B2© and copper sulphate as starting material. The influence of the incubation time and the effect of biosynthesis Cu-NPs on the growth of bacteria and yeast were studied in this research. The direct and rapid formation of Cu-NPs occurs when Rhodotorula diobovata interacts with Cu+2 ions, which is visually indicated by a color transition from light blue to dark bluish-green. UV-Vis, FT-IR, DLS and zeta-potential, TEM, and EDX were used to characterize the shape, size, and structure of the produced Cu-NPs. The UV-Vis measurement revealed a surface plasmon resonance (SPR) peak at 606.5 and 622.5 nm due to the capping activity of Rhodotorula diobovata biomolecules in the synthesis of Cu-NPs. Energy dispersive spectroscopy (EDX) revealed low-intensity peaks of phosphorus (P), calcium (Ca), and potassium (K) elements and high-intensity metallic peaks of copper (Cu), oxygen (O), and sulfur (S). The synthesis of Cu-NPs is triggered by the presence of functional groups -OH, C=C, and C-H, as proven by FT-IR analysis. The TEM investigations also showed that the particles ranged in size from 33 to 63 nm and had a spherical form. DLS and zeta-potential analysis indicate that the particle sizes are 22.1 nm and 19.5 nm, and the zeta-potentials are -26.8 and -17.3, confirming the stability of the Cu-NPs. The antibacterial activity of Cu-NPs against Staphylococcus aureus from diabetic foot infection and E. coli and Candida albicans from vaginal swabs was demonstrated. The produced Cu-NPs' hemolytic activity was comparable to that of the control Ringer solution. Nanotechnology antibiotic resistance copper nanoparticles green synthesis Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6738954","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":462183002,"identity":"db84e57e-97b3-4eec-a253-b2e407baa0f1","order_by":0,"name":"Batool M. 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This research aimed to explore the eco-friendly, straightforward, and cost-effective green synthesis of copper nanoparticles (Cu-NPs) using Rhodotorula diobovata Strain B2© and copper sulphate as starting material. The influence of the incubation time and the effect of biosynthesis Cu-NPs on the growth of bacteria and yeast were studied in this research. The direct and rapid formation of Cu-NPs occurs when Rhodotorula diobovata interacts with Cu+2 ions, which is visually indicated by a color transition from light blue to dark bluish-green. UV-Vis, FT-IR, DLS and zeta-potential, TEM, and EDX were used to characterize the shape, size, and structure of the produced Cu-NPs. The UV-Vis measurement revealed a surface plasmon resonance (SPR) peak at 606.5 and 622.5 nm due to the capping activity of Rhodotorula diobovata biomolecules in the synthesis of Cu-NPs. Energy dispersive spectroscopy (EDX) revealed low-intensity peaks of phosphorus (P), calcium (Ca), and potassium (K) elements and high-intensity metallic peaks of copper (Cu), oxygen (O), and sulfur (S). The synthesis of Cu-NPs is triggered by the presence of functional groups -OH, C=C, and C-H, as proven by FT-IR analysis. The TEM investigations also showed that the particles ranged in size from 33 to 63 nm and had a spherical form. DLS and zeta-potential analysis indicate that the particle sizes are 22.1 nm and 19.5 nm, and the zeta-potentials are -26.8 and -17.3, confirming the stability of the Cu-NPs. The antibacterial activity of Cu-NPs against Staphylococcus aureus from diabetic foot infection and E. coli and Candida albicans from vaginal swabs was demonstrated. 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