A prospective study on evaluation of Novel Immunochromatographic card for rapid detection of Five carbapenemases enzymes in metallobetalactamase producers.

2025 · doi:10.1099/acmi.0.001024.v1
preprint OA: closed
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Abstract

Objective: To evaluate the performance of a novel immunochromatographic card test (TRURAPID® O.K.N.V.I. RESIST-5) for rapid detection of five carbapenemase enzymes in metallo-beta-lactamase (MBL) producing organisms, compared to real-time PCR and an advanced expert system.

Methods

Isolated clinically 100 non-duplicates of multidrug-resistant Gram-negative bacilli (GNB) expressing MBL production were tested using the novel IC card, real-time PCR, and the Vitek-2 Advanced Expert System (AES). Sensitivity, specificity, and turnaround time were evaluated.

Results

The novel IC card showed high sensitivity for detecting NDM (93%) and KPC (91.7%) carbapenemases, but lower sensitivity for OXA-48 (60%), VIM (67%), and IMP (33%) compared to PCR. It had a rapid turnaround time of 15-20 minutes versus 5-7 hours for PCR and 18-22 hours for AES.

Conclusion

The novel IC card offers a rapid, cost-effective approach for detecting carbapenemases, particularly NDM and KPC, in clinical microbiology practice. It may be beneficial in resource-limited settings where these enzymes are prevalent. Further evaluation is needed to assess its role as a screening or confirmatory test, especially during nosocomial outbreaks. - Received: - Version Posted: Funding - Dr. Ram Manohar Lohia Institute of Medical Sciences - Principal Award Recipient: Jyotsna Agarwal

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