Mechanisms on the Combined Remediation of Sulfonylurea Herbicides Contaminated Soil by Chenggangzhangella sp. BI-1 and Cucumber

Mechanisms on the Combined Remediation of Sulfonylurea Herbicides Contaminated Soil by Chenggangzhangella sp. BI-1 and Cucumber | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Mechanisms on the Combined Remediation of Sulfonylurea Herbicides Contaminated Soil by Chenggangzhangella sp. BI-1 and Cucumber Lian-zheng Dong, Zi-wang Yuan, Xue Xia, Zi-yue Ding, Yuan-yuan Zhao, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8546370/v1 This work is licensed under a CC BY 4.0 License Status: Under Revision Version 1 posted 6 You are reading this latest preprint version Abstract Sulfonylurea herbicides, widely used in agriculture, persisted long in soil, has raised considerable environmental concerns. Microbial remediation is effective in decomposing organic pollutants. However, researches on microbe-plant combined remediation of contaminated soil based on rhizosphere environment remains limited. In this study, the influence of cucumber root exudates on degrading strain Chenggangzhangella sp. BI-1 was investigated. Additionally, the treatment of cucumber seeds coating with strain BI-1 was employed to study its effects on cucumber stress resistance, herbicides degradation in rhizosphere soil, and the structure of the bacterial community. The results showed that root exudates significantly enhanced BI-1 degradation efficiency towards tribenuron-methyl, chlorimuron-ethyl, and bensulfuron-methyl. Liquid chromatography-mass spectrometry analysis identified 15 amino acids, 55 organic acids, and 4 vitamins in root exudates. Among these, lysine, glutamic acid, vitamin C, succinic acid, and tartaric acid significantly promoted colonization of strain BI-1 on roots. Transcriptomic analysis revealed that 1524 genes in strain BI-1 were significantly upregulated by root exudates, while 1643 genes downregulated, associated with nutrient metabolism, chemotaxis and genetic information processing. After coating treatment under the optimal conditions of 0.8 g·10 mL-1 carboxymethyl cellulose sodium, inoculation-seeds ratio of 1:35 and coating time of 57 min, cucumber seeds were planted in soil contaminated (3.0 mg/kg), the degradation rates reached 75.3% (tribenuron-methyl), 71.3% (chlorimuron-ethyl), and 77.7% (bensulfuron-methyl) after 14 d, which were significantly higher than those in the non-coating treatment. Meanwhile, the coating treatment reduced the malondialdehyde content in cucumber leaves and increased the activities of peroxidase, superoxide dismutase and catalase. It also significantly altered the relative abundances of cucumber rhizosphere bacteria, improving the richness and diversity of bacterial community. Functional prediction via FAPROTAX indicated that the coating treatment increased the relative abundance of functions such as aerobic chemoheterotrophy, thereby facilitating herbicides degradation. This study could provide a basis for the bioremediation of contaminated soils. Sulfonylurea herbicides Chenggangzhangella sp. BI-1 Root exudates Seed coating Bacterial community Full Text Additional Declarations Table 1 and 2 are available in the Supplementary Files section. Supplementary Files Tables.docx Supplementarymaterials.docx Cite Share Download PDF Status: Under Revision Version 1 posted Editorial decision: Major Revision 08 May, 2026 Reviewers agreed at journal 01 Feb, 2026 Reviewers invited by journal 19 Jan, 2026 Editor invited by journal 14 Jan, 2026 Editor assigned by journal 13 Jan, 2026 First submitted to journal 13 Jan, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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