plot2DO: a tool to assess the quality and distribution of genomic data

Summary Micrococcal nuclease digestion followed by deep sequencing (MNase-seq) is the most used method to investigate nucleosome organization on a genome-wide scale. We present plot2DO , a software package for creating 2D occupancy plots, which allows biologists to evaluate the quality of MNase-seq data and to visualize the distribution of nucleosomes near the functional regions of the genome ( e.g . gene promoters, origins of replication, etc.). Availability And Implementation The plot2DO open source package is freely available on GitHub at https://github.com/rchereji/plot2DO under the MIT license. Contact [email protected] Supplementary Information Supplementary data are available at Bioinformatics online.