Co-culture of intact Peritoneum Explants and Endometrial Tissue

Objective:To establish the model of co-culture tissue of peritoneum and endometrium in vitro for the study of endometriosis.Methods:1.Explants of intact human peritoneum from the anterior abdominal wall were cultured with whole fragments of mechanically dispersed endometrium.2.Adhesion of endometrial fragments was studied by histologic examination and scaning electron microscopy.Results:1.After incubation,the mesothelium was in most areas,immunohistochemical staining revealed that cytokeratin vimentin were still present in the cell cytoplasm.2.Endometrium was identified attached to the surface of the peritoneum.Concluslons:Explants of intact human peritoneum issuitable for the model of tissue culture.