Two conserved transcription factors and a histone deubiquitinase regulate the mitochondrial unfolded protein response and longevity interacting with insulin signalling

Two conserved transcription factors and a histone deubiquitinase regulate the mitochondrial unfolded protein response and longevity interacting with insulin signalling | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Two conserved transcription factors and a histone deubiquitinase regulate the mitochondrial unfolded protein response and longevity interacting with insulin signalling Sergio Gordillo-García*, Jesús Fernandez-Abascal*, Blanca Hernando-Rodríguez, and 10 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3337719/v2 This work is licensed under a CC BY 4.0 License Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Abstract The mitochondrial prohibitin (PHB) complex is critical to mitochondrial function. Depletion of PHB has opposite effects on ageing, shortening lifespan in wild-type worms while extending the lifespan of metabolically compromised animals, including insulin/IGF-1 receptor daf-2(e1370) mutants. PHB loss strongly induces the mitochondrial unfolded protein response (UPRmt) to maintain mitochondrial homeostasis, but this response is attenuated in daf-2 mutants. To uncover new regulators of this differential PHB-mediated stress response, as well as mechanisms behind the opposite longevity outcomes of PHB depletion, we carry out the first genome-wide double RNAi screen in C. elegans. We identify two transcription factors, ZNF-622 and TLF-1, and the histone deubiquitinase USP-48 as modulators of the UPRmt and longevity interacting with insulin signalling. We further show that USP-48 interacts with DVE-1 to regulate the UPRmt mostly independently of ATFS-1 through deubiquitylation of histone H2B. Collectively, this study proves the feasibility of double RNAi genetic screens and underlines the importance of epigenetic regulation in mitochondrial stress mediated longevity. *Sergio Gordillo-García and Jesús Fernandez-Abascal contributed equally. Biological sciences/Cell biology/Cell signalling/Stress signalling Biological sciences/Genetics/Gene expression Full Text Additional Declarations There is NO Competing Interest. Supplementary Files TableS1.pdf Lifespan Table TableS2.pdf Retested candidates FileS1.xlsx Screening raw data FileS2.xlsx Analysed screening data FileS3.xlsx Gene Ontology analysis FileS4.xlsx qRT-PCR data FileS5.xlsx RNAseq_Gene overlap Cite Share Download PDF Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3337719","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":560558553,"identity":"26065770-1c8f-42ec-a40f-0b8e0cfc91cc","order_by":0,"name":"Sergio Gordillo-García*","email":"","orcid":"","institution":"Andalusian Centre for Developmental Biology, Consejo Superior de Investigaciones Científicas/Junta de Andalucía/Universidad Pablo de Olavide, Seville, Spain; Department of Molecular Biology and Biochemical Engineering, Universidad Pablo de Olavide, Seville, Spain","correspondingAuthor":false,"prefix":"","firstName":"Sergio","middleName":"","lastName":"Gordillo-García*","suffix":""},{"id":560558554,"identity":"39a4725b-a1d9-4768-88ba-17a1062750ea","order_by":1,"name":"Jesús Fernandez-Abascal*","email":"","orcid":"https://orcid.org/0000-0002-9558-8331","institution":"Andalusian Centre for Developmental Biology, Consejo Superior de Investigaciones Científicas/Junta de Andalucía/Universidad Pablo de Olavide, Seville, Spain; Department of Molecular Biology and Biochemical Engineering, Universidad Pablo de Olavide, Seville, Spain","correspondingAuthor":false,"prefix":"","firstName":"Jesús","middleName":"","lastName":"Fernandez-Abascal*","suffix":""},{"id":560558555,"identity":"320a5fba-61c0-43b1-a4df-aaf506948c82","order_by":2,"name":"Blanca Hernando-Rodríguez","email":"","orcid":"https://orcid.org/0000-0002-1148-1255","institution":"Andalusian Centre for Developmental Biology, Consejo Superior de Investigaciones Científicas/Junta de Andalucía/Universidad Pablo de Olavide, Seville, Spain; Department of Molecular Biology and Biochemical Engineering, Universidad Pablo de Olavide, Seville, Spain","correspondingAuthor":false,"prefix":"","firstName":"Blanca","middleName":"","lastName":"Hernando-Rodríguez","suffix":""},{"id":560558556,"identity":"a3a00776-778a-4669-a454-13e2eca13231","order_by":3,"name":"María Jesús Rodríguez-Palero","email":"","orcid":"","institution":"Andalusian Centre for Developmental Biology, Consejo Superior de Investigaciones Científicas/Junta de Andalucía/Universidad Pablo de Olavide, Seville, Spain; Department of Molecular Biology and Biochemical Engineering, Universidad Pablo de Olavide, Seville, Spain","correspondingAuthor":false,"prefix":"","firstName":"María","middleName":"Jesús","lastName":"Rodríguez-Palero","suffix":""},{"id":560558558,"identity":"a7bb2ff0-abe4-4341-a4f7-0e5210fecb34","order_by":4,"name":"Laura Cantalejo-Carrasco","email":"","orcid":"","institution":"Andalusian Centre for Developmental Biology, Consejo Superior de Investigaciones Científicas/Junta de Andalucía/Universidad Pablo de Olavide, Seville, Spain; Department of Molecular Biology and Biochemical Engineering, Universidad Pablo de Olavide, Seville, Spain","correspondingAuthor":false,"prefix":"","firstName":"Laura","middleName":"","lastName":"Cantalejo-Carrasco","suffix":""},{"id":560558559,"identity":"704857da-0a1c-47ea-af4f-518ebd20532e","order_by":5,"name":"Aitor Jarit-Cabanillas","email":"","orcid":"","institution":"Andalusian Centre for Developmental Biology, Consejo Superior de Investigaciones Científicas/Junta de Andalucía/Universidad Pablo de Olavide, Seville, Spain; Department of Molecular Biology and Biochemical Engineering, Universidad Pablo de Olavide, Seville, Spain","correspondingAuthor":false,"prefix":"","firstName":"Aitor","middleName":"","lastName":"Jarit-Cabanillas","suffix":""},{"id":560558560,"identity":"ff935b93-b3b3-4fca-858f-81216e5a6a2f","order_by":6,"name":"Manuel D. 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