The Gfr uptake system provides a context-dependent fitness advantage to Salmonella Typhimurium SL1344 during the initial gut colonization phase

The Gfr uptake system provides a context-dependent fitness advantage to Salmonella Typhimurium SL1344 during the initial gut colonization phase | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Molecular Microbiology This is a preprint and has not been peer reviewed. Data may be preliminary. 6 May 2025 V1 Latest version Share on The Gfr uptake system provides a context-dependent fitness advantage to Salmonella Typhimurium SL1344 during the initial gut colonization phase Authors : Lea Fuchs , Cora Lisbeth Dieterich , Elena Melgarejo Ros , Philipp Keller , Leanid Laganenka , Christopher Schubert , Julia A. Vorholt , Jörn Piel , Wolf-Dietrich Hardt , and Bidong D. Nguyen 0009-0009-8201-0387 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174657243.38353949/v1 Published Molecular Microbiology Version of record Peer review timeline 265 views 67 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Salmonella enterica serovar Typhimurium ( S . Tm) is a major cause of foodborne diarrhea. However, in healthy individuals, the microbiota typically restricts the growth of incoming pathogens, a protective mechanism termed colonization resistance (CR). To circumvent CR, Salmonella strains can utilize private nutrients that remain untapped by the resident microbiota. However, the metabolic pathways and environmental niches promoting pathogen growth are still not completely understood. Here, we investigate the significance of the gfr operon in gut colonization of S. Tm, which is essential for the utilization of fructoselysine (FL) and glucoselysine (GL). These Amadori compounds are present in heated foods with high protein and carbohydrate contents, particularly in Western-type diets. We detected FL in both mouse chow and the intestinal tract of mice and showed that gfr mutants are attenuated during the initial phase of colonization in the murine model. Experiments in gnotobiotic mice and competition experiments with Escherichia coli suggest that gfr -dependent fitness advantage is context-dependent. We conclude that dietary Amadori products like FL can support S . Tm gut colonization, depending on the metabolic capacities of the microbiota. Supplementary Material File (all figures v3.pdf) Download 2.69 MB File (fuchs et al 2025 v3.docx) Download 179.36 KB Information & Authors Information Version history V1 Version 1 06 May 2025 Peer review timeline Published Molecular Microbiology Version of Record 13 Oct 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection Molecular Microbiology Keywords amadori products colonization resistance diet fructoselysine/glucoselysine microbial competition Authors Affiliations Lea Fuchs Eidgenossische Technische Hochschule Zurich Institut fur Mikrobiologie View all articles by this author Cora Lisbeth Dieterich Eidgenossische Technische Hochschule Zurich Institut fur Mikrobiologie View all articles by this author Elena Melgarejo Ros Eidgenossische Technische Hochschule Zurich Institut fur Mikrobiologie View all articles by this author Philipp Keller Eidgenossische Technische Hochschule Zurich Institut fur Mikrobiologie View all articles by this author Leanid Laganenka Eidgenossische Technische Hochschule Zurich Institut fur Mikrobiologie View all articles by this author Christopher Schubert Eidgenossische Technische Hochschule Zurich Institut fur Mikrobiologie View all articles by this author Julia A. Vorholt Eidgenossische Technische Hochschule Zurich Institut fur Mikrobiologie View all articles by this author Jörn Piel Eidgenossische Technische Hochschule Zurich Institut fur Mikrobiologie View all articles by this author Wolf-Dietrich Hardt Eidgenossische Technische Hochschule Zurich Institut fur Mikrobiologie View all articles by this author Bidong D. Nguyen 0009-0009-8201-0387 [email protected] Eidgenossische Technische Hochschule Zurich Institut fur Mikrobiologie View all articles by this author Metrics & Citations Metrics Article Usage 265 views 67 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Lea Fuchs, Cora Lisbeth Dieterich, Elena Melgarejo Ros, et al. The Gfr uptake system provides a context-dependent fitness advantage to Salmonella Typhimurium SL1344 during the initial gut colonization phase. Authorea . 06 May 2025. DOI: https://doi.org/10.22541/au.174657243.38353949/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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