In Vivo Nanobubble-Enhanced Vascular Imaging with High-Frame-Rate Plane-Wave Ultrasound

In Vivo Nanobubble-Enhanced Vascular Imaging with High-Frame-Rate Plane-Wave Ultrasound | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 22 December 2025 V1 Latest version Share on In Vivo Nanobubble-Enhanced Vascular Imaging with High-Frame-Rate Plane-Wave Ultrasound Authors : Yu Weng 0009-0009-3973-1349 [email protected] , Niloufar R Shirazi , Steven Tran , Eno Hysi , Agata A Exner , and Michael C Kolios Authors Info & Affiliations https://doi.org/10.22541/au.176642533.39377025/v1 278 views 130 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Nanobubbles (NBs) have emerged as promising ultrasound contrast agents for enhancing vascular and extravascular imaging. This work presents the first in vivo demonstration of NB-enhanced vascular ultrasound imaging in a preclinical setting, achieved by combining plane-wave imaging (PWI) with single-pulse transmission and high-frame-rate acquisition. The method was implemented on the VisualSonics Vevo F2 system operated in Vevo Advanced Data Acquisition (VADA) mode, which enables full customization of pulse sequences and access to raw radiofrequency (RF) data. Two techniques, adjacent frame differencing (AFD) and singular value decomposition (SVD), were applied to extract NB responses from tissue clutter. While SVD yielded stronger clutter suppression after breathing cycle segmentation, it was computationally expensive. When the frame rate was sufficiently high, AFD achieved comparable performance to SVD with significantly faster processing, making it more suitable for real-time implementation. In vivo studies on mouse kidneys demonstrated vascular visualization within seconds after NB injection. Additional studies on mouse liver and transcranial brain imaging further showcased the framework's robustness and generalizability. Results show that high-frame-rate PWI effectively mitigates motion artifacts without requiring multi-pulse transmission, such as amplitude modulation (AM) or pulse inversion (PI), to enhance nonlinear contrast. This enables robust power Doppler visualization of NB responses and flow dynamics within the vasculature. Overall, this work establishes a foundation for in vivo NB-enhanced vascular ultrasound imaging and provides strong potential for real-time preclinical applications and future clinical translation. Supplementary Material File (in vivo nb-enhanced vascular imaging_techrxiv_compressed.pdf) Download 8.96 MB Information & Authors Information Version history V1 Version 1 22 December 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords adjacent frame differencing high-framerate ultrasound nanobubble-enhanced ultrasound imaging singular value decomposition vascular imaging Authors Affiliations Yu Weng 0009-0009-3973-1349 [email protected] View all articles by this author Niloufar R Shirazi View all articles by this author Steven Tran View all articles by this author Eno Hysi View all articles by this author Agata A Exner View all articles by this author Michael C Kolios View all articles by this author Metrics & Citations Metrics Article Usage 278 views 130 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Yu Weng, Niloufar R Shirazi, Steven Tran, et al. In Vivo Nanobubble-Enhanced Vascular Imaging with High-Frame-Rate Plane-Wave Ultrasound. Authorea . 22 December 2025. DOI: https://doi.org/10.22541/au.176642533.39377025/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. 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