Towards understanding mare endometrosis: ex vivo study on the role of relaxin
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An ex vivo study on mare endometrosis reveals that relaxin modulates inflammatory cytokines, progesterone levels, and fibrotic protein abundance, indicating potential for treating equine endometrial fibrosis.
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Abstract
CONTEXT: Mare endometrosis remains a poorly understood pathological process.
AIMS: Mare endometrial tissue with endometrosis was used to determine the effects of relaxin (RLX). Healthy tissues that were left untreated (H), or treated with vehicle (Hveh), and tissues with endometrosis that were left untreated (E), treated with vehicle (Eveh), and treated with RLX (10, 25, 50 nM; ER) were used for an ex vivo system for 72 h.
METHODS: Tissue histological examination, and immunoenzymatic measurement of the concentrations of transforming growth factor β (TGF-β), interleukins (IL-6 and IL-8), and progesterone were performed. Western blotting was used to study the abundance of the following proteins involved in cellular processes, signaling, and interactions: N-cadherin, cortactin, Wnt/β-catenin signaling with kinases: glycogen synthase kinase-3β (GSK-3β), protein kinase B (AKT), metalloproteinases: MMP9, MMP2, and cyclins: D1, D3.
KEY RESULTS: RLX (50 nM) decreased the concentration of TGF-β, increased concentrations of IL-6 and IL-8, and decreased the concentration of progesterone, without histological alterations of the treated tissues. Interactions of RLX with proteins showed changes in protein abundance, as follows: N-cadherin increased, cortactin increased, β-catenin, GSK-3β and AKT showed an increase of phosphorylation, MMP2 and MMP9 increased, and cyclin D3 increased in ER versus E.
CONCLUSIONS: Results indicated that RLX exerted both anti-/pro-inflammatory as well as anti-/pro-fibrotic effects depending on interacting cytokine/protein.
IMPLICATIONS: In equine breeding, the application of RLX with marker protein antagonists/agonists may be promising in endometrial fibrosis treatment.
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Towards understanding mare endometrosis : ex vivo study on the role of relaxin
Journal
Reproduction, Fertility and Development
140
Author
Volume
37
Number
18
Article ID
RD25166
ISSN
1031-3613
eISSN
1448-5990
Access date
2025-11-28
Remarks
Online First 2025-11-19
Language
English
Journal language
English
Affiliation
Wydział Lekarski : Zakład Biochemii Nowotworów
| dc.affiliation | Wydział Lekarski : Zakład Biochemii Nowotworów | |
| dc.cm.idOmega | UJCM764185c535054e2c82a4dc3464935551 | pl |
| dc.contributor.author | Profaska, Magdalena | |
| dc.contributor.author | Zarzycka, Marta - 104151 | |
| dc.contributor.author | Dubniewicz, Klaudia | |
| dc.contributor.author | Witkowski, Maciej - 173982 | |
| dc.contributor.author | Wieczorek, Jaroslaw | |
| dc.contributor.author | Gil, Dorota - 129497 | |
| dc.contributor.author | Wafula, Samwel | |
| dc.contributor.author | Lanh, Do Kim | |
| dc.contributor.author | Kotula-Balak, Malgorzata | |
| dc.date.accession | 2025-11-28 | pl |
| dc.date.accessioned | 2025-11-30T23:30:42Z | |
| dc.date.available | 2025-11-30T23:30:42Z | |
| dc.date.createdat | 2025-11-30T23:30:42Z | en |
| dc.date.issued | 2025 | pl |
| dc.description.additional | Online First 2025-11-19 | pl |
| dc.description.number | 18 | |
| dc.description.volume | 37 | |
| dc.identifier.articleid | RD25166 | |
| dc.identifier.doi | 10.1071/rd25166 | pl |
| dc.identifier.eissn | 1448-5990 | pl |
| dc.identifier.issn | 1031-3613 | pl |
| dc.identifier.uri | https://ruj.uj.edu.pl/handle/item/566385 | |
| dc.language | eng | pl |
| dc.language.container | eng | pl |
| dc.pbn.affiliation | Dziedzina nauk medycznych i nauk o zdrowiu : nauki medyczne | |
| dc.rights | Dodaję tylko opis bibliograficzny | * |
| dc.rights.licence | Bez licencji otwartego dostępu | pl |
| dc.source.integrator | false | |
| dc.subtype | Article | pl |
| dc.title | Towards understanding mare endometrosis : ex vivo study on the role of relaxin | |
| dc.title.journal | Reproduction, Fertility and Development | pl |
| dc.type | JournalArticle | pl |
| dspace.entity.type | Publication |
dc.affiliation
Wydział Lekarski : Zakład Biochemii Nowotworów dc.cm.idOmegapl
UJCM764185c535054e2c82a4dc3464935551 dc.contributor.author
Profaska, Magdalena dc.contributor.author
Zarzycka, Marta - 104151 dc.contributor.author
Dubniewicz, Klaudia dc.contributor.author
Witkowski, Maciej - 173982 dc.contributor.author
Wieczorek, Jaroslaw dc.contributor.author
Gil, Dorota - 129497 dc.contributor.author
Wafula, Samwel dc.contributor.author
Lanh, Do Kim dc.contributor.author
Kotula-Balak, Malgorzata dc.date.accessionpl
2025-11-28 dc.date.accessioned
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RD25166 dc.identifier.doipl
10.1071/rd25166 dc.identifier.eissnpl
1448-5990 dc.identifier.issnpl
1031-3613 dc.identifier.uri
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Dziedzina nauk medycznych i nauk o zdrowiu : nauki medyczne dc.rights*
Dodaję tylko opis bibliograficzny dc.rights.licencepl
Bez licencji otwartego dostępu dc.source.integrator
false dc.subtypepl
Article dc.title
Towards understanding mare endometrosis : ex vivo study on the role of relaxin dc.title.journalpl
Reproduction, Fertility and Development dc.typepl
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Publication Affiliations
Wydział Lekarski
Zarzycka, Marta
Gil, Dorota
No affiliation
Profaska, Magdalena
Dubniewicz, Klaudia
Witkowski, Maciej
Wieczorek, Jaroslaw
Wafula, Samwel
Lanh, Do Kim
Kotula-Balak, Malgorzata
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