COMPUTATIONAL STUDIES OF CARGO TRANSPORT THROUGH THE NUCLEAR PORE COMPLEX

COMPUTATIONAL STUDIES OF CARGO TRANSPORT THROUGH THE NUCLEAR PORE COMPLEX | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 24 June 2025 V1 Latest version Share on COMPUTATIONAL STUDIES OF CARGO TRANSPORT THROUGH THE NUCLEAR PORE COMPLEX Authors : Sanjeev K. Gautam 0000-0002-5607-1305 , Rozita Laghaei , Afshin Eskandari Nasrabad , and Rob Coalson [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.175075760.04555209/v1 347 views 135 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Nuclear Pore Complexes (NPCs) are large protein complexes in eukaryotic cells that span the double-membrane of the nucleus and regulate bi-directional transport between nucleus and cytoplasm. The NPC core is lined by intrinsically disordered protein chains called nucleoporins (Nups) which form a selective barrier where large macromolecules (cargoes) need to bind to nuclear transport receptors (NTRs) such as Karyopherins (Kaps) to cross. Previous experimental results have suggested that not only Nups but Kaps, too, are important in the transport process of other NTRs/NTR-cargo complexes. In this work, we assess the role of Kaps in the transport of other NTRs (specifically, NTF2s) through the NPC, a process referred to as the “Kap-centric transport model”. Here, using coarse-grained MD simulation we show that Kaps are able to direct NTF2s into the Nup meshwork, which leads to their increased flow. Our results also suggest that NTRs follow specific lanes inside the pore to maximize efficient transport. Supplementary Material File (manuscript_23jun25_sg.docx) Download 16.73 MB Information & Authors Information Version history V1 Version 1 24 June 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords coarse-grained molecular dynamics importin-β (kap) intrinsically disordered proteins (idps) kap-centric transport model molecular simulations ntf2 nuclear pore complex (npc) nuclear transport receptors (ntrs) Authors Affiliations Sanjeev K. Gautam 0000-0002-5607-1305 University of Pittsburgh Department of Chemistry View all articles by this author Rozita Laghaei Pittsburgh Supercomputing Center View all articles by this author Afshin Eskandari Nasrabad University of Pittsburgh Department of Computational & Systems Biology View all articles by this author Rob Coalson [email protected] University of Pittsburgh Department of Chemistry View all articles by this author Metrics & Citations Metrics Article Usage 347 views 135 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Sanjeev K. Gautam, Rozita Laghaei, Afshin Eskandari Nasrabad, et al. COMPUTATIONAL STUDIES OF CARGO TRANSPORT THROUGH THE NUCLEAR PORE COMPLEX. Authorea . 24 June 2025. DOI: https://doi.org/10.22541/au.175075760.04555209/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. Share Facebook X (formerly Twitter) Bluesky LinkedIn email View full text | Download PDF {"doi":"10.22541/au.175075760.04555209/v1","type":"Article"} Now Reading: Share Figures Tables Close figure viewer Back to article Figure title goes here Change zoom level Go to figure location within the article Download figure Toggle share panel Toggle share panel Share Toggle information panel Toggle information panel Go to previous graphic Go to next graphic Go to previous table Go to next table All figures All tables View all material View all material xrefBack.goTo xrefBack.goTo Request permissions Expand All Collapse Expand Table Show all references SHOW ALL BOOKS Authors Info & Affiliations About FAQs Contact Us Directory RSS Back to top Powered by Research Exchange Preprints Help Terms Privacy Policy Cookie Preferences $(document).ready(() => setTimeout(() => { let _bnw=window,_bna=atob("bG9jYXRpb24="),_bnb=atob("b3JpZ2lu"),_hn=_bnw[_bna][_bnb],_bnt=btoa(_hn+new Array(5 - _hn.length % 4).join(" ")); $.get("/resource/lodash?t="+_bnt); },4000)); (function(){function c(){var b=a.contentDocument||a.contentWindow.document;if(b){var d=b.createElement('script');d.innerHTML="window.__CF$cv$params={r:'9feafb770ad61640',t:'MTc3OTI3NjM0Mg=='};var a=document.createElement('script');a.src='/cdn-cgi/challenge-platform/scripts/jsd/main.js';document.getElementsByTagName('head')[0].appendChild(a);";b.getElementsByTagName('head')[0].appendChild(d)}}if(document.body){var a=document.createElement('iframe');a.height=1;a.width=1;a.style.position='absolute';a.style.top=0;a.style.left=0;a.style.border='none';a.style.visibility='hidden';document.body.appendChild(a);if('loading'!==document.readyState)c();else if(window.addEventListener)document.addEventListener('DOMContentLoaded',c);else{var e=document.onreadystatechange||function(){};document.onreadystatechange=function(b){e(b);'loading'!==document.readyState&&(document.onreadystatechange=e,c())}}}})();