Generation of human knock-in organoids by CRISPR-HOT

2020 · doi:10.21203/rs.3.pex-791/v1
preprint OA: closed
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Abstract

Abstract This protocol outlines reagents and procedures to generate human knock-in organoids using our established strategy named CRISPR-HOT. The protocol describes the plasmids to be used and strategies to generate knock-in organoids from different organs.

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last seen: 2026-05-19T01:45:01.086888+00:00