A Comprehensive Investigation of Glycoprotein-based Nucleic Acid Vaccines for Hantaan Virus

A Comprehensive Investigation of Glycoprotein-based Nucleic Acid Vaccines for Hantaan Virus | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article A Comprehensive Investigation of Glycoprotein-based Nucleic Acid Vaccines for Hantaan Virus kun yang, Jiaxing zhang, Junqi Zhang, Yanbo Wang, Yubo Sun, Yongkai Wang, and 15 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3933421/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 23 Oct, 2024 Read the published version in npj Vaccines → Version 1 posted 10 You are reading this latest preprint version Abstract Hemorrhagic fever with renal syndrome (HFRS) occurs throughout Eurasia with considerable morbidity and mortality. Currently, the absence of specific treatments or effective antiviral drugs for hantavirus infection makes developing safe and effective vaccines a high priority. Here, we report the development of three novel nucleic acid vaccine candidates, mRNA, DNA, and DNA encapsulated in lipid nanoparticles, encoding the glycoproteins of the Hantaan virus (HTNV). To comprehensively evaluate the potential of candidate HTNV nucleic acid vaccines in preventing HFRS, we focus on evaluating their immunogenicity and efficacy in mice and comparing them with an inactivated vaccine as the benchmark. Our findings reveal that all candidate vaccines activated instant and sustained immune responses, offering effective in vivo protection. Notably, the nucleic acid vaccines demonstrated distinct advantages over the inactivated vaccine, in both humoral and cellular immunity. These results mark a significant step in developing nucleic acid vaccines for HTNV, suggesting that sequential immunization with DNA and mRNA vaccines could further amplify the advantages of nucleic acid vaccines. Full Text Additional Declarations (Not answered) Supplementary Files SupplementalMaterial.pdf Cite Share Download PDF Status: Published Journal Publication published 23 Oct, 2024 Read the published version in npj Vaccines → Version 1 posted Editorial decision: revise 08 May, 2024 Review # 3 received at journal 15 Mar, 2024 Review # 1 received at journal 13 Mar, 2024 Reviewer # 3 agreed at journal 11 Mar, 2024 Reviewer # 2 agreed at journal 07 Mar, 2024 Reviewer # 1 agreed at journal 04 Mar, 2024 Reviewers invited by journal 04 Mar, 2024 Editor assigned by journal 20 Feb, 2024 Submission checks completed at journal 06 Feb, 2024 First submitted to journal 06 Feb, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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