A Comparative Study on the Effect of Vitamin B6 and Cabergoline in Preventing Risperidone-Induced Hyperprolactinemia in Adolescent Female Wistar Rats

A Comparative Study on the Effect of Vitamin B6 and Cabergoline in Preventing Risperidone-Induced Hyperprolactinemia in Adolescent Female Wistar Rats | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Data Note A Comparative Study on the Effect of Vitamin B6 and Cabergoline in Preventing Risperidone-Induced Hyperprolactinemia in Adolescent Female Wistar Rats Sahithi Gopu, krishna teja vemulaghat, Aswin prabhu, varun kumar This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7668253/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Context: Hyperprolactinemia is a common side effect of antipsychotic medications, including risperidone, resulting from the blockade of dopamine receptors in the hypothalamus. This condition can lead to various complications, emphasizing the need for effective preventive measures. Objective: This study aimed to investigate the efficacy of Vitamin B6 supplementation and compare it with Cabergoline administration as a preventive measure for risperidone-induced hyperprolactinemia. Design, Setting, Patients, and Intervention: An experimental study was conducted using inbred adolescent female Wistar rats. The rats were divided into four groups: Control, Standard (risperidone only), Test 1 (risperidone + Vitamin B6), and Test 2 (risperidone + Cabergoline). The interventions were administered orally for 14 days. Outcome Measures: The primary outcome measure was the serum prolactin level, assessed using the Elisa method and the Elabscience Rat PRL Elisa Kit. Results: The results showed that Vitamin B6 and Cabergoline significantly reduced serum prolactin levels compared to the standard group (p < 0.05). However, Vitamin B6 supplementation exhibited a slightly greater reduction (60%) than Cabergoline (75%). Conclusion: Vitamin B6 supplementation may be an effective preventive measure for risperidone-induced hyperprolactinemia. It demonstrated comparable efficacy to Cabergoline but with the advantage of being more affordable and having fewer side effects. Further research is warranted to validate these findings in human populations and explore the feasibility of Vitamin B6 as a prophylactic measure for antipsychotic-induced hyperprolactinemia. Laboratory Diagnostics Hyperprolactinemia risperidone Cabergoline Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7668253","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Data Note","associatedPublications":[],"authors":[{"id":518334050,"identity":"c471f78c-3b5d-4c95-8880-417545b03b6e","order_by":0,"name":"Sahithi Gopu","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABBUlEQVRIie2PMWrDMBiF5aXTfwAVQ3UFhYLIYJyxh8giI3AWt3MLoaiLPYWs6SGylo4ShkyKvQqyNHsH9wKlqp1AF9sdC9U3iDe8j6cfIY/nTwJIBRJ3md9H7g2e1Kiiz0pj0m9FjipIyy4Gz3nZhkGFFHtdfrxOEblZ7UK4qONtUbqVZTTvU6i540objCamSkOAg3gxiVN26a3sU1BGlc6dsslYCPggmHJKIMtehazffyq0Eqw+DivInlYIztjlhquY2ZEVat3KPsdAoRLTRgnOrFvhA7eQdXbdPOSPV6RYaZt8xjNWL45vzTLq/9gJoArakLRNPlLv5mSnzH5T9ng8nv/FF+OoaA/Hj92QAAAAAElFTkSuQmCC","orcid":"","institution":"Osmania Medical College, Hyderabad","correspondingAuthor":true,"prefix":"","firstName":"Sahithi","middleName":"","lastName":"Gopu","suffix":""},{"id":518334051,"identity":"f64e155e-d738-46e7-b4e9-83f5fbdcdf3a","order_by":1,"name":"krishna teja vemulaghat","email":"","orcid":"","institution":"osmania medical collge","correspondingAuthor":false,"prefix":"","firstName":"krishna","middleName":"teja","lastName":"vemulaghat","suffix":""},{"id":518334052,"identity":"0fb00a45-6622-4e67-93aa-51244135b858","order_by":2,"name":"Aswin prabhu","email":"","orcid":"","institution":"madurai medical college","correspondingAuthor":false,"prefix":"","firstName":"Aswin","middleName":"","lastName":"prabhu","suffix":""},{"id":518334053,"identity":"eeba0f77-6784-43af-bb6f-7ea9eccc9900","order_by":3,"name":"varun kumar","email":"","orcid":"","institution":"madurai medical college","correspondingAuthor":false,"prefix":"","firstName":"varun","middleName":"","lastName":"kumar","suffix":""}],"badges":[],"createdAt":"2025-09-21 17:59:44","currentVersionCode":1,"declarations":{"humanSubjects":true,"vertebrateSubjects":true,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":true,"humanSubjectConsent":true,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":true},"doi":"10.21203/rs.3.rs-7668253/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7668253/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":91949334,"identity":"55d23901-643e-4381-a582-fd0833cd8145","added_by":"auto","created_at":"2025-09-23 06:17:20","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":294940,"visible":true,"origin":"","legend":"","description":"","filename":"ratfinalproject.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7668253/v1_covered_2e00b70f-2f07-4922-baa0-14d6c6ccc9db.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003e\u003cstrong\u003eA Comparative Study on the Effect of Vitamin B6 and Cabergoline in Preventing Risperidone-Induced Hyperprolactinemia in Adolescent Female Wistar Rats\u003c/strong\u003e\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Hyperprolactinemia, risperidone, Cabergoline ","lastPublishedDoi":"10.21203/rs.3.rs-7668253/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7668253/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eContext:\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eHyperprolactinemia is a common side effect of antipsychotic medications, including risperidone, resulting from the blockade of dopamine receptors in the hypothalamus. This condition can lead to various complications, emphasizing the need for effective preventive measures.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eObjective:\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis study aimed to investigate the efficacy of Vitamin B6 supplementation and compare it with Cabergoline administration as a preventive measure for risperidone-induced hyperprolactinemia.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eDesign, Setting, Patients, and Intervention:\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAn experimental study was conducted using inbred adolescent female Wistar rats. The rats were divided into four groups: Control, Standard (risperidone only), Test 1 (risperidone + Vitamin B6), and Test 2 (risperidone + Cabergoline). The interventions were administered orally for 14 days.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eOutcome Measures:\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe primary outcome measure was the serum prolactin level, assessed using the Elisa method and the Elabscience Rat PRL Elisa Kit.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults:\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe results showed that Vitamin B6 and Cabergoline significantly reduced serum prolactin levels compared to the standard group (p \u0026lt; 0.05). However, Vitamin B6 supplementation exhibited a slightly greater reduction (60%) than Cabergoline (75%).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusion:\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eVitamin B6 supplementation may be an effective preventive measure for risperidone-induced hyperprolactinemia. It demonstrated comparable efficacy to Cabergoline but with the advantage of being more affordable and having fewer side effects. Further research is warranted to validate these findings in human populations and explore the feasibility of Vitamin B6 as a prophylactic measure for antipsychotic-induced hyperprolactinemia.\u003c/p\u003e","manuscriptTitle":"A Comparative Study on the Effect of Vitamin B6 and Cabergoline in Preventing Risperidone-Induced Hyperprolactinemia in Adolescent Female Wistar Rats","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-09-23 06:09:12","doi":"10.21203/rs.3.rs-7668253/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"4dfb02b4-718d-410f-a1d9-3d97d5c9a494","owner":[],"postedDate":"September 23rd, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":55079736,"name":"Laboratory Diagnostics"}],"tags":[],"updatedAt":"2025-09-23T06:09:13+00:00","versionOfRecord":[],"versionCreatedAt":"2025-09-23 06:09:12","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7668253","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7668253","identity":"rs-7668253","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}