The MYCN/miR-20a-5p/NFKBIB/NF-κB signaling axis promotes the proliferation, invasion and migration of neuroblastoma

Purpose: To investigate the effect of MYCN on the proliferation, invasion and migration of NB and its molecular mechanism. Methods We first compared the expression level of MYCN mRNA in NB cells by PCR. Next, the expression level of miR-20a-5p in NB cells was detected by PCR. Then, cell counting kit-8, wound healing and transwell assays were used to determine the ability of proliferation, migration and invasion of NB cells. Both cells were infected with miR-20a-5p lentivirus particles, and then the infection efficiency was detected by PCR. Repeat the above cell function test to determine the proliferation, migration, invasion and apoptosis of NB cells after overexpression or low expression of miR-20a-5p. Bioinformatics analysis was used to predict the binding sites of miR-20a-5p and NFKBIB. A double luciferase reporting assay was used to verify the targeting relationship between miR-20a-5p and NFIBIB. WB confirmed that miR-20a-5p could activate the NF- κB pathway. Results MYCN and miR-20a-5p can promote the proliferation, invasion and migration of NB cells, and inhibit NFKBIB by upregulating miR-20a-5p, thereby activating the NF- κB pathway. Conclusion In this study, we speculate that the MYCN/miR-20a-5p/NFKBIB/NF- κ B signaling axis can promote the proliferation, invasion and migration of NB cells.