Vitamin D supplementation and its effect on the expression of genes associated with inflammation and its pathophysiological outcomes in Gestational Diabetic women: a case-control study in Ekiti State, Nigeria

Vitamin D supplementation and its effect on the expression of genes associated with inflammation and its pathophysiological outcomes in Gestational Diabetic women: a case-control study in Ekiti State, Nigeria | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Vitamin D supplementation and its effect on the expression of genes associated with inflammation and its pathophysiological outcomes in Gestational Diabetic women: a case-control study in Ekiti State, Nigeria Ayobami Sunday Arulogun, Modupe F Asaolu, Ibikunle Akinlua This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9093903/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background: Gestational diabetes mellitus (GDM) has become a global issue affecting a significant population of pregnant women worldwide with high maternal and infant mortality. It is characterized by glucose intolerance during pregnancy which could be linked to inflammation. This study was designed to investigate the modulating effects of vitamin D supplementation on the expression of inflammatory marker genes namely: IL-8, 1L-10 and IL-1β and β –Actin in gestational diabetic women in Ekiti state, Nigeria. Method: A total of 150 participants aged 18-40 was recruited from Ekiti State University Teaching Hospital, Ado Ekiti, Ekiti State. They were grouped into 3; (50 glucose tolerant, 50 GDM women and 50 GDM women on vitamin D supplementation. They were given vitamin D supplementation, 1000 IU/day after 24 th week for 8 weeks. Blood samples were taken from each participant and processed to obtain the plasma; gene expression of inflammatory marker genes were performed on the plasma using standard methods. Results: The result shows a significant increase (p<0.05) in the expression of IL-8, 1L-10 and IL-1β genes in GDM women when compared with the control. However, after vitamin D supplementation, significant decrease in the expression of these genes was observed in these patients (GDM women on supplementation) compared with GDM women not on supplementation. Conclusion: This significant decrease shows that vitamin D could modulate inflammatory processes in these patients and help in the prevention and management of gestational diabetes mellitus and its complications. Trial Registration : Not applicable Ethical Approval : EKSUTH/A65/2024/03/010 Gestational Diabetes Vitamin D Inflammation Vitamin D supplementation Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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It is characterized by glucose intolerance during pregnancy which could be linked to inflammation. This study was designed to investigate the modulating effects of vitamin D supplementation on the expression of inflammatory marker genes namely: IL-8, 1L-10 and IL-1β and β –Actin in gestational diabetic women in Ekiti state, Nigeria.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethod:\u003c/strong\u003e A total of 150 participants aged 18-40 was recruited from Ekiti State University Teaching Hospital, Ado Ekiti, Ekiti State. They were grouped into 3; (50 glucose tolerant, 50 GDM women and 50 GDM women on vitamin D supplementation. They were given vitamin D supplementation, 1000 IU/day after 24\u003csup\u003eth\u003c/sup\u003e week for 8 weeks. Blood samples were taken from each participant and processed to obtain the plasma; gene expression of inflammatory marker genes were performed on the plasma using standard methods.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults:\u003c/strong\u003e The result shows a significant increase (p\u0026lt;0.05) in the expression of IL-8, 1L-10 and IL-1β genes in GDM women when compared with the control. 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