Improve CTAB method for DNA isolation of Vanda orchids

Background: Growing interest in molecular studies and bioinformatics increases in demand for good quality DNA extraction techniques. Results: : We demonstrate an easy and rapid DNA extraction process by modifying many existing ones. The present study focuses on isolation of clean and pure DNAs from the leave explants of Vanda testacea, Vanda ampullacea var. auranticum and Vanda PB Mazumder. Demonstration of the purity of the isolated DNAs is obtained through reproducible PCR amplification with several random primers using 25µl reaction mixture. Conclusion: The improved CTAB DNA extraction method are practically suited for PCR analysis and this technique is simple, rapid and efficient, and can be used in analysis in situations where large numbers of samples are to be analysed.