Secreted glycoside hydrolase BcGH61 from Botrytis cinerea induces cell death by the apoplastic location and triggers intracellular immune perception

Secreted glycoside hydrolase BcGH61 from Botrytis cinerea induces cell death by the apoplastic location and triggers intracellular immune perception | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 10 July 2025 V1 Latest version Share on Secreted glycoside hydrolase BcGH61 from Botrytis cinerea induces cell death by the apoplastic location and triggers intracellular immune perception Authors : Wenjun Zhu , Ziyao Wang , Min Fang , Binbin Huang , Xiaofei Nie , Yong Liang 0000-0003-0612-1594 , Kai Bi 0000-0002-3375-682X [email protected] , and Can Zheng Authors Info & Affiliations https://doi.org/10.22541/au.175212400.01341418/v1 Published Molecular Plant Pathology Version of record Peer review timeline 351 views 148 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Cell death-inducing proteins (CDIPs) are critical mediators of infection in the necrotrophic pathogen Botrytis cinerea , enabling host colonization across its broad plant host range. Here, we identified a novel plant CDIP from B. cinerea , BcGH61, which exhibits dual activity: triggering extensive plant cell death while simultaneously promoting host defense responses. Functional analysis demonstrates that its full cell death-inducing capacity requires its apoplastic compartmentalization, intact glycoside hydrolase activity, two conserved cysteine residues, and specific structural motifs spanning residues 193-240. Notably, this phytotoxic activity operates through a mechanism independent of the canonical immune coreceptors BAK1 and SOBIR1. During pathogenic invasion, bcgh61 demonstrates upregulated expression. While the bcgh61 gene deletion mutant displays no significant developmental defects, it exhibits severely attenuated virulence, establishing BcGH61 as an essential pathogenicity factor. Furthermore, we identified NbHrBP1, a Nicotiana benthamiana hypersensitive response-binding protein, as a direct interactor of BcGH61 during infection, functioning in intracellular immune perception but not in cell death induction. Taken together, our results provide evidence that BcGH61 is a novel critical virulence factor for establishing infection in host plant by its phytotoxic activity and underscore the role of NbHrBP1-based plant surveillance system in recognizing fungal secreted proteins as a pivotal defense strategy against necrotrophic pathogens. Supplementary Material File (fig-1.emf) Download 48.84 MB File (fig-2.emf) Download 12.67 MB File (fig-3.emf) Download 58.06 MB File (fig-4.emf) Download 9.52 MB File (fig-5.emf) Download 8.51 MB File (fig-6.emf) Download 3.01 MB File (fig-7.emf) Download 10.57 MB File (fig-8.emf) Download 33.79 MB File (fig-9.emf) Download 2.91 MB File (manuscript-pce.docx) Download 49.24 MB Information & Authors Information Version history V1 Version 1 10 July 2025 Peer review timeline Published Molecular Plant Pathology Version of Record 30 Dec 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords botrytis cinerea bcgh61 nbhrbp1 pathogenicity signaling transcriptome Authors Affiliations Wenjun Zhu Wuhan Polytechnic University View all articles by this author Ziyao Wang Wuhan Polytechnic University View all articles by this author Min Fang Wuhan Polytechnic University View all articles by this author Binbin Huang Wuhan Polytechnic University View all articles by this author Xiaofei Nie Wuhan Polytechnic University View all articles by this author Yong Liang 0000-0003-0612-1594 Tel Aviv University View all articles by this author Kai Bi 0000-0002-3375-682X [email protected] Wuhan Polytechnic University View all articles by this author Can Zheng Wuhan Polytechnic University View all articles by this author Metrics & Citations Metrics Article Usage 351 views 148 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Wenjun Zhu, Ziyao Wang, Min Fang, et al. Secreted glycoside hydrolase BcGH61 from Botrytis cinerea induces cell death by the apoplastic location and triggers intracellular immune perception. Authorea . 10 July 2025. DOI: https://doi.org/10.22541/au.175212400.01341418/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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