Escherichia coli (ATCC 25922) induced corrosion in Zn-Ni-Cu and Zn-Ni-Cu- TiB 2 coated ASTM A-36 Steel

Escherichia coli (ATCC 25922) induced corrosion in Zn-Ni-Cu and Zn-Ni-Cu- TiB 2 coated ASTM A-36 Steel | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Escherichia coli (ATCC 25922) induced corrosion in Zn-Ni-Cu and Zn-Ni-Cu- TiB 2 coated ASTM A-36 Steel Sheikh Idrees Ali, sheikh nazir This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4428787/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The study aimed to investigate the corrosion performance of Zn-Ni-Cu and Zn-Ni-Cu-TiB 2 coatings in the microbial-induced environment (E-Coli, ATCC 25922, and 3.5%NaCl solution). Zn-Ni-Cu and Zn-Ni-Cu-TiB 2 were surfaces coated on an ASTM A-36 Steel substrate utilizing a high-velocity oxy-fuel (HVOF) thermal spray process. Immersion tests following ASTM G-31, and ASTM G1-03, standards were performed in Escherichia Coli (E-Coli, American Type Culture CollectionATCC25922) bacteria medium.The effect of Zn, Ni, and Ti was studied in preventing microbial-induced corrosion.SEM, and XRD analysis before and after helped to understand the morphological and structural changes in coated/uncoated ASTM A-36 steel. Various forms of rust were ascertained in XRD analysis. The presence of Zn and Cu prevented the bacterial attachment with coated surface and hence prevented the underlying substrate from being corroded substantially.The coatings performed well and limited the growth of bacteria. The uncoated ASTM A-36 Steel specimen showed well-developed bacterial colonies on the surface and in the solution medium. All forms of rust were reported in XRD analysis for uncoated ASTM A-36 steel while few forms of rust were reported in coated ASTM A-36 steel.Tafel polarization and electrochemical impedance spectroscopy (EIS) in Escherichia Coli (E-Coli, ATCC 25922) medium confirmed that coated samples were more corrosion resistant than uncoated ASTM A-36 Steel specimens because of the corrosion potential (Ecorr) values of both coated samples were higher than uncoated ASTM A-36 Steel suggesting better anodic protection. The corrosion current density (Icorr) of both coated samples (Zn-Ni-Cu and Zn-Ni-Cu-TiB 2 ) were lower than uncoated ASTM A-36 Steel specimens also inferring better performance.Results of the current study concluded that Zn-Ni-Cu-TiB 2 coated ASTM A-36 Steel was able to enhance the microbial-induced corrosion resistance without deteriorating the microstructural, adhesion strength, and other properties of the coating. The mass loss for both Zn-Ni-Cu and Zn-Ni-Cu-TiB 2 coatings was 88% less compared to uncoated ASTM A-36 Steel. It is seen from scanning electron micrographs that the coatings produced were well-developed with minimum pores and cracks. After immersion cycle tests none of the coatings showed delamination or microbial colony attachment to the surface compared to ASTM A-36 Steel wherein degradation of the whole surface is visible along with corrosion in the interface. Escherichia Coli bacteria High-velocity oxy-fuel spraying Microbial induced corrosion Reactive oxygen species Biotic environment Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4428787","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":305613857,"identity":"d046c588-bc4f-4ccf-b406-4fbf84207ab5","order_by":0,"name":"Sheikh Idrees Ali","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA50lEQVRIiWNgGAWjYBAC+RlsbAwMBswgNuMDIMHDR0iLwQ2EFmYDkBY2glokQFoYwFrYJMAkQS3SbWkPGAqs8/nZ259Vfs2xk2FjYH746AY+v8w5dtyAwSDdcmbPGbPbstuSgQ5jMzbOwWfNjfQ2CQaDwwYGN3LYbktuYwZq4WGTJkqL/f3nz4olt9UToyXtGMQWCQYzxo/bDhPWYnAjLU0iwSDdQOJMjrE047bjPGzMBPwiPyPNTOLDH2sD/vbjDz/+3FZtz8/e/PAxXoeBQAKUZuYBk4SUIwPGH6SoHgWjYBSMghEDAK8IQJVC8C5aAAAAAElFTkSuQmCC","orcid":"","institution":"National Institute of Technology Srinagar","correspondingAuthor":true,"prefix":"","firstName":"Sheikh","middleName":"Idrees","lastName":"Ali","suffix":""},{"id":305613858,"identity":"8457dd39-d7a0-457d-88cf-d728228c0479","order_by":1,"name":"sheikh nazir","email":"","orcid":"","institution":"National Institute of Technology Srinagar","correspondingAuthor":false,"prefix":"","firstName":"sheikh","middleName":"","lastName":"nazir","suffix":""}],"badges":[],"createdAt":"2024-05-16 06:16:42","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4428787/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4428787/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":59055124,"identity":"769059ea-f3fe-4419-bd1f-f21faeaec99d","added_by":"auto","created_at":"2024-06-25 20:46:30","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":2192281,"visible":true,"origin":"","legend":"","description":"","filename":"biocorrosionpaper.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4428787/v1_covered_fea485d7-e7e0-407d-acab-83906a58a808.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Escherichia coli (ATCC 25922) induced corrosion in Zn-Ni-Cu and Zn-Ni-Cu- TiB 2 coated ASTM A-36 Steel","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Escherichia Coli bacteria, High-velocity oxy-fuel spraying, Microbial induced corrosion, Reactive oxygen species, Biotic environment","lastPublishedDoi":"10.21203/rs.3.rs-4428787/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4428787/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThe study aimed to investigate the corrosion performance of Zn-Ni-Cu and Zn-Ni-Cu-TiB\u003csub\u003e2\u003c/sub\u003e coatings in the microbial-induced environment (E-Coli, ATCC 25922, and 3.5%NaCl solution). Zn-Ni-Cu and Zn-Ni-Cu-TiB\u003csub\u003e2\u003c/sub\u003e were surfaces coated on an ASTM A-36 Steel substrate utilizing a high-velocity oxy-fuel (HVOF) thermal spray process. Immersion tests following ASTM G-31, and ASTM G1-03, standards were performed in Escherichia Coli (E-Coli, American Type Culture CollectionATCC25922) bacteria medium.The effect of Zn, Ni, and Ti was studied in preventing microbial-induced corrosion.SEM, and XRD analysis before and after helped to understand the morphological and structural changes in coated/uncoated ASTM A-36 steel. Various forms of rust were ascertained in XRD analysis. The presence of Zn and Cu prevented the bacterial attachment with coated surface and hence prevented the underlying substrate from being corroded substantially.The coatings performed well and limited the growth of bacteria. The uncoated ASTM A-36 Steel specimen showed well-developed bacterial colonies on the surface and in the solution medium. All forms of rust were reported in XRD analysis for uncoated ASTM A-36 steel while few forms of rust were reported in coated ASTM A-36 steel.Tafel polarization and electrochemical impedance spectroscopy (EIS) in Escherichia Coli (E-Coli, ATCC 25922) medium confirmed that coated samples were more corrosion resistant than uncoated ASTM A-36 Steel specimens because of the corrosion potential (Ecorr) values of both coated samples were higher than uncoated ASTM A-36 Steel suggesting better anodic protection. The corrosion current density (Icorr) of both coated samples (Zn-Ni-Cu and Zn-Ni-Cu-TiB\u003csub\u003e2\u003c/sub\u003e) were lower than uncoated ASTM A-36 Steel specimens also inferring better performance.Results of the current study concluded that Zn-Ni-Cu-TiB\u003csub\u003e2\u003c/sub\u003e coated ASTM A-36 Steel was able to enhance the microbial-induced corrosion resistance without deteriorating the microstructural, adhesion strength, and other properties of the coating. The mass loss for both Zn-Ni-Cu and Zn-Ni-Cu-TiB\u003csub\u003e2\u003c/sub\u003e coatings was 88% less compared to uncoated ASTM A-36 Steel. It is seen from scanning electron micrographs that the coatings produced were well-developed with minimum pores and cracks. After immersion cycle tests none of the coatings showed delamination or microbial colony attachment to the surface compared to ASTM A-36 Steel wherein degradation of the whole surface is visible along with corrosion in the interface.\u003c/p\u003e","manuscriptTitle":"Escherichia coli (ATCC 25922) induced corrosion in Zn-Ni-Cu and Zn-Ni-Cu- TiB 2 coated ASTM A-36 Steel","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-05-24 17:10:45","doi":"10.21203/rs.3.rs-4428787/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"62b6d0cb-a6cd-4418-b27e-2f52e80a4d24","owner":[],"postedDate":"May 24th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-11-20T09:08:42+00:00","versionOfRecord":[],"versionCreatedAt":"2024-05-24 17:10:45","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4428787","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4428787","identity":"rs-4428787","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}