Polygonati Rhizoma reduces inflammation and improves muscle atrophy and fibrosis in sarcopenia through the EGFR/MAPK pathway

Polygonati Rhizoma reduces inflammation and improves muscle atrophy and fibrosis in sarcopenia through the EGFR/MAPK pathway | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 18 March 2025 V1 Latest version Share on Polygonati Rhizoma reduces inflammation and improves muscle atrophy and fibrosis in sarcopenia through the EGFR/MAPK pathway Authors : Zhongyuan Liu , Zining Huang , Yang Li , and Zhiwen Zhang 0000-0002-3303-9046 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174229427.73222692/v1 186 views 126 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Polygonati Rhizoma, a traditional Chinese medicine, demonstrates therapeutic potential for sarcopenia, though its bioactive components and mechanisms remain unclear. Ultra-high-performance liquid chromatography-tandem mass spectrometry identified 826 compounds in Polygonati Rhizoma extract(PRE), with Genistein, Camptothecin, and Chrysoeriol highlighted as key bioactive constituents. Network pharmacology analysis revealed critical targets including EGFR, TNF, and CTNNB1, along with associated pathways such as MAPK, IL-17, and TNF signaling. Molecular docking confirmed strong binding affinities between these compounds and targets, with binding energies below -5 kcal/mol. In a D-galactose-induced sarcopenia mouse model, PRE administered at 60 and 120 mg/kg for 8 weeks improved exercise capacity, reduced muscle atrophy and fibrosis through histopathological assessments, and suppressed inflammatory markers. Mechanistic studies demonstrated that the extract enhanced EGFR phosphorylation, activated MAPK signaling, and inhibited TNF and CTNNB1-mediated fibrotic pathways. Pathway analysis further linked these effects to MAPK, IL-17, and TNF signaling cascades, underscoring their roles in mitigating inflammation and fibrosis. This study identifies Genistein, Camptothecin, and Chrysoeriol as pivotal components of Polygonati Rhizoma, acting through multi-target and multi-pathway mechanisms to alleviate sarcopenia. The findings provide a scientific foundation for its clinical application in treating muscle atrophy disorders. Supplementary Material File (figure 1.tif) Download 6.68 MB File (figure 2.tif) Download 17.70 MB File (figure 3.tif) Download 6.49 MB File (figure 4.tif) Download 14.76 MB File (figure 5.tif) Download 2.07 MB File (figure 6.tif) Download 34.58 MB File (figure legends.docx) Download 11.62 KB File (manuscript.docx) Download 148.75 KB File (table 1.docx) Download 96.39 KB File (table s1.docx) Download 11.80 KB File (table2.docx) Download 16.50 KB File (table3.docx) Download 22.50 KB Information & Authors Information Version history V1 Version 1 18 March 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords atrophy inflammation molecular docking network pharmacology polygonati rhizoma sarcopenia Authors Affiliations Zhongyuan Liu Hubei University of Traditional Chinese Medicine View all articles by this author Zining Huang Hubei University School of Life Sciences View all articles by this author Yang Li Affiliated Hospital of Hubei University of Traditional Chinese Medicine View all articles by this author Zhiwen Zhang 0000-0002-3303-9046 [email protected] Affiliated Hospital of Hubei University of Traditional Chinese Medicine View all articles by this author Metrics & Citations Metrics Article Usage 186 views 126 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Zhongyuan Liu, Zining Huang, Yang Li, et al. Polygonati Rhizoma reduces inflammation and improves muscle atrophy and fibrosis in sarcopenia through the EGFR/MAPK pathway. Authorea . 18 March 2025. 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