The PFT gene in the Fhb1 QTL is not required for wheat Fusarium head blight resistance

The PFT gene in the Fhb1 QTL is not required for wheat Fusarium head blight resistance | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Brief Communication The PFT gene in the Fhb1 QTL is not required for wheat Fusarium head blight resistance Zhengqiang Ma, Jinxin Shi, Zhongxin Kong, Jinkun Zhong, Xiaoqiu Zhang, and 10 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3710199/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 14 Jan, 2025 Read the published version in Nature Genetics → Version 1 posted You are reading this latest preprint version Abstract The ambiguity about whether the histidine-rich calcium-binding protein-coding gene ( His R ) or the pore-forming toxin-like gene ( PFT ) or both are responsible for wheat Fusarium head blight (FHB) resistance conferred by Fhb1 QTL hinders wheat improvement and resistance mechanism elucidation. Here, with a series of developed lines possessing His R but varying in PFT presence and five His R -carrying PFT mutant lines created via gene editing, we show that PFT does not confer FHB resistance and the His R resistance effect does not require PFT . We also show that PFT is not associated with morphological and phenological characters that often affect FHB severity. Biological sciences/Genetics/Plant genetics Biological sciences/Plant sciences/Plant breeding Full Text Additional Declarations There is NO Competing Interest. Supplementary Files Suplementarytablesandnotes.pdf Suplementary tables and notes Cite Share Download PDF Status: Published Journal Publication published 14 Jan, 2025 Read the published version in Nature Genetics → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3710199","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Brief Communication","associatedPublications":[],"authors":[{"id":268649398,"identity":"10cfad42-9379-43d0-982b-75d8ca704178","order_by":0,"name":"Zhengqiang 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Here, with a series of developed lines possessing \u003cem\u003eHis\u003c/em\u003e\u003csup\u003eR\u003c/sup\u003e but varying in \u003cem\u003ePFT \u003c/em\u003epresence and five \u003cem\u003eHis\u003c/em\u003e\u003csup\u003eR\u003c/sup\u003e-carrying \u003cem\u003ePFT \u003c/em\u003emutant lines created via gene editing, we show that \u003cem\u003ePFT\u003c/em\u003e does not confer FHB resistance and the \u003cem\u003eHis\u003c/em\u003e\u003csup\u003eR\u003c/sup\u003e resistance effect does not require \u003cem\u003ePFT\u003c/em\u003e. We also show that \u003cem\u003ePFT\u003c/em\u003e is not associated with morphological and phenological characters that often affect FHB severity.\u003c/p\u003e","manuscriptTitle":"The PFT gene in the Fhb1 QTL is not required for wheat Fusarium head blight resistance","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-01-24 03:39:42","doi":"10.21203/rs.3.rs-3710199/v1","editorialEvents":[],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"nature-genetics","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"ng","sideBox":"Learn more about [Nature Genetics](http://www.nature.com/ng/)","snPcode":"","submissionUrl":"","title":"Nature Genetics","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"ejp","reportingPortfolio":"Nature Research","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"1b8735dd-ada2-4798-81b8-25bea0c6b446","owner":[],"postedDate":"January 24th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":28300092,"name":"Biological sciences/Genetics/Plant genetics"},{"id":28300093,"name":"Biological sciences/Plant sciences/Plant breeding"}],"tags":[],"updatedAt":"2025-01-15T08:10:03+00:00","versionOfRecord":{"articleIdentity":"rs-3710199","link":"https://doi.org/10.1038/s41588-024-02056-3","journal":{"identity":"nature-genetics","isVorOnly":false,"title":"Nature Genetics"},"publishedOn":"2025-01-14 05:00:00","publishedOnDateReadable":"January 14th, 2025"},"versionCreatedAt":"2024-01-24 03:39:42","video":"","vorDoi":"10.1038/s41588-024-02056-3","vorDoiUrl":"https://doi.org/10.1038/s41588-024-02056-3","workflowStages":[]},"version":"v1","identity":"rs-3710199","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3710199","identity":"rs-3710199","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}