Accuracy study of colloidal gold-based rapid test reagents for Chinese food

Accuracy study of colloidal gold-based rapid test reagents for Chinese food | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Accuracy study of colloidal gold-based rapid test reagents for Chinese food Ye Gu, Cai-Hong Huang, Yi-Ke Du, Yue Li, Yu Huan This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7696075/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 11 You are reading this latest preprint version Abstract Objective To evaluate the accuracy of food rapid test reagents based on colloidal gold technology for the detection of chloramphenicol, enrofloxacin and sulfonamides in livestock, poultry and aquatic products, and to provide a reference for their practical application. Methods Rapid test reagents from different manufacturers were used to detect the above targets in pork, chicken, fish, shrimp, and other samples. The specificity, linearity and sensitivity of the reagents were analysed by setting up a multi-concentration gradient combined with the interference experiment of flavouring matrix. Results Reagents D and E showed strong anti-interference ability and specificity for chloramphenicol, though reagent E yielded false positives at 0.05 µg/kg. For enrofloxacin, polynomial fitting correlation coefficients (0.9991, 0.9966, 0.9993) outperformed linear regression. Compared to reagents using sulfadiazine or sulfamethoxazole as the benchmark, those based on sulfamethazine demonstrated superior accuracy. Reagent B achieved an overall accuracy rate of 96.6%. In contrast, reagents using sulfamethoxazole as the benchmark showed no response to sulfadiazine, sulfamerazine, or sulfamethazine, resulting in a false negative rate as high as 100%. Conclusion The colloidal gold detection technology is effective in screening for chloramphenicol in seasoned meat and fish samples; for enrofloxacin, it exhibits a good linear relationship and is suitable for preliminary screening in the circulation chain. The sensitivity for sulfonamides depends heavily on the benchmark substance used in reagent design. We recommend selecting rapid tests developed with a structurally representative benchmark, like sulfamethazine, and validating them across matrices before deployment. Confirm with traditional methods if needed. Rapid Test Chloramphenicol Enrofloxacin Sulfonamides Specificity Linearity Sensitivity Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 31 Oct, 2025 Reviews received at journal 31 Oct, 2025 Reviews received at journal 21 Oct, 2025 Reviewers agreed at journal 17 Oct, 2025 Reviews received at journal 16 Oct, 2025 Reviewers agreed at journal 13 Oct, 2025 Reviewers agreed at journal 12 Oct, 2025 Reviewers invited by journal 12 Oct, 2025 Editor assigned by journal 30 Sep, 2025 Submission checks completed at journal 30 Sep, 2025 First submitted to journal 23 Sep, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7696075","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":533513827,"identity":"7eeadbb4-e064-4cac-befc-c474b04064b3","order_by":0,"name":"Ye Gu","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA1klEQVRIie3RsQqCUBTG8SvBaTnkeiXQJwhuBIKLz3JEcPIB3DICJ6M5CHqFIHC+Idhiu2MSNLW01VRrTd62oPvff3A+DmM63Q8G/fn+RAlMl/25VCMDrEJxqgfGKq9Ijdg8dq02s41tEwvFw1hEnGq3Z6Xxrbky3x6lnaSUgpIITHbceWsWTlzZRYyMiOoKrdmiGCKTQdFJeihkkD25KPGiSADHaZCBEBWCIkEIGdVAVg4Tby0Utjib8+FxT4BM59w218S3O8lHHBVf806+FTqdTvcXvQD0nUNYwa935QAAAABJRU5ErkJggg==","orcid":"","institution":"Wuxi Food Safety Inspection and Test Center","correspondingAuthor":true,"prefix":"","firstName":"Ye","middleName":"","lastName":"Gu","suffix":""},{"id":533513828,"identity":"67785324-a684-4ad8-9c61-84c5bf3270b4","order_by":1,"name":"Cai-Hong Huang","email":"","orcid":"","institution":"Jiangnan University","correspondingAuthor":false,"prefix":"","firstName":"Cai-Hong","middleName":"","lastName":"Huang","suffix":""},{"id":533513829,"identity":"c7ea5dd5-69a9-41c0-bac9-343deec47646","order_by":2,"name":"Yi-Ke Du","email":"","orcid":"","institution":"Technological University of the Shannon","correspondingAuthor":false,"prefix":"","firstName":"Yi-Ke","middleName":"","lastName":"Du","suffix":""},{"id":533513830,"identity":"e0311293-7fc8-4ecf-a227-16f6085c36ee","order_by":3,"name":"Yue Li","email":"","orcid":"","institution":"Wuxi Food Safety Inspection and Test Center","correspondingAuthor":false,"prefix":"","firstName":"Yue","middleName":"","lastName":"Li","suffix":""},{"id":533513831,"identity":"71bdc750-c281-449b-ac4a-b4eddad6dd30","order_by":4,"name":"Yu Huan","email":"","orcid":"","institution":"SCIEX Analytical Instrument Trading Co., Ltd","correspondingAuthor":false,"prefix":"","firstName":"Yu","middleName":"","lastName":"Huan","suffix":""}],"badges":[],"createdAt":"2025-09-23 15:38:19","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7696075/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7696075/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":94489726,"identity":"6e2c8b71-c7c1-4599-a26c-52d3bf2e8708","added_by":"auto","created_at":"2025-10-27 17:05:46","extension":"docx","order_by":0,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":1236995,"visible":true,"origin":"","legend":"","description":"","filename":"Accuracystudyofcolloidalgoldbasedrapidtestreagents.docx","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/16e6a442357483ece497e58b.docx"},{"id":94437485,"identity":"486d93c0-dd08-4a82-a202-356460d6d77b","added_by":"auto","created_at":"2025-10-27 14:21:00","extension":"json","order_by":1,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":6830,"visible":true,"origin":"","legend":"","description":"","filename":"b042eb145c364b5093a9f7d80952b5d1.json","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/e22130f8ff5c7f14757d547e.json"},{"id":94438788,"identity":"6e782506-c8e5-4bba-83db-d6acddd39561","added_by":"auto","created_at":"2025-10-27 14:21:57","extension":"xml","order_by":2,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":143667,"visible":true,"origin":"","legend":"","description":"","filename":"b042eb145c364b5093a9f7d80952b5d11enriched.xml","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/0ba0129c5e5997fea150e8ab.xml"},{"id":94438724,"identity":"fffdbc83-044f-464f-8127-38cb15d2664c","added_by":"auto","created_at":"2025-10-27 14:21:42","extension":"jpeg","order_by":3,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":1074,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/b6545ccd62e1ad3dc479f9a5.jpeg"},{"id":94438586,"identity":"11529458-0a32-4037-80de-587365fa05c1","added_by":"auto","created_at":"2025-10-27 14:21:35","extension":"jpeg","order_by":4,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":1074,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/97a7d6daeed7f2743111da64.jpeg"},{"id":94438013,"identity":"a21e3e94-8dc8-4665-b4c6-cf9d0916bc0c","added_by":"auto","created_at":"2025-10-27 14:21:17","extension":"jpeg","order_by":5,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":1074,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/780f768d28979ce6a01d4def.jpeg"},{"id":94438743,"identity":"9a1e057e-6f2a-4eb8-b9d5-938a3a4b0e3c","added_by":"auto","created_at":"2025-10-27 14:21:43","extension":"jpeg","order_by":6,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":4542,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage4.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/d24e00b77732d5b4cc86a912.jpeg"},{"id":94438766,"identity":"e8474b91-c975-4ae1-a746-9c28d7e9b26a","added_by":"auto","created_at":"2025-10-27 14:21:49","extension":"jpeg","order_by":7,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":232239,"visible":true,"origin":"","legend":"","description":"","filename":"floatimage5.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/2e5cf8e01d1f9fcc6b5f16d6.jpeg"},{"id":94436888,"identity":"e69e7155-aac3-45f6-b9c9-f4c0e85cde76","added_by":"auto","created_at":"2025-10-27 14:20:49","extension":"jpeg","order_by":8,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":36257,"visible":true,"origin":"","legend":"","description":"","filename":"groupimage1.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/ceb3fd34a676e2bed5728190.jpeg"},{"id":94438744,"identity":"5bc7a74d-642b-4e2d-b878-d662b4e39cd2","added_by":"auto","created_at":"2025-10-27 14:21:44","extension":"jpeg","order_by":9,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":32399,"visible":true,"origin":"","legend":"","description":"","filename":"groupimage2.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/f781bf158d85f6bc04bb1852.jpeg"},{"id":94438635,"identity":"2c317817-6316-4f56-9a3f-17b890f23a6b","added_by":"auto","created_at":"2025-10-27 14:21:38","extension":"jpeg","order_by":10,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":18534,"visible":true,"origin":"","legend":"","description":"","filename":"groupimage3.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/0da931039c679742089d235b.jpeg"},{"id":94438585,"identity":"2e22cad1-06a8-4e7e-97fe-09685d389a3b","added_by":"auto","created_at":"2025-10-27 14:21:35","extension":"jpeg","order_by":11,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":57166,"visible":true,"origin":"","legend":"","description":"","filename":"groupimage4.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/64ba8a635b65c1a7da113d36.jpeg"},{"id":94438808,"identity":"0c2194e6-886d-4174-9752-116b21c309a7","added_by":"auto","created_at":"2025-10-27 14:22:01","extension":"jpeg","order_by":12,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":23970,"visible":true,"origin":"","legend":"","description":"","filename":"groupimage5.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/b41f82c00034a1b24189d394.jpeg"},{"id":94438000,"identity":"fea29b33-56e3-424d-b79b-749fff432a0e","added_by":"auto","created_at":"2025-10-27 14:21:16","extension":"jpeg","order_by":13,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":25065,"visible":true,"origin":"","legend":"","description":"","filename":"groupimage6.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/a95ec7d3fbf353d975608b1c.jpeg"},{"id":94438807,"identity":"db4882fe-f189-469c-b3c0-219556d08099","added_by":"auto","created_at":"2025-10-27 14:22:01","extension":"jpeg","order_by":14,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":16339,"visible":true,"origin":"","legend":"","description":"","filename":"groupimage7.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/3d4795a1c88ed19c22c77b25.jpeg"},{"id":94436868,"identity":"ebe25dea-a64c-4d62-a2fb-06f87bbcbc61","added_by":"auto","created_at":"2025-10-27 14:20:49","extension":"png","order_by":15,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":935,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/a7334d3d90da09bf52d4e5b2.png"},{"id":94438637,"identity":"c98bd246-1a30-43a9-b765-fec2a71ae2b2","added_by":"auto","created_at":"2025-10-27 14:21:38","extension":"png","order_by":16,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":935,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage2.png","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/8b7d102ecf7b809e97d34dca.png"},{"id":94437382,"identity":"20ae1d44-f8a2-40d3-8b95-901683a2a881","added_by":"auto","created_at":"2025-10-27 14:20:57","extension":"png","order_by":17,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":935,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage2.png","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/8a1b1ea6b8e935ec0fde3791.png"},{"id":94438285,"identity":"d00d0188-4b4f-4f84-a51a-21f064f51d4b","added_by":"auto","created_at":"2025-10-27 14:21:27","extension":"png","order_by":18,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":1140,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage4.png","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/dabe38a9180257385fd04d50.png"},{"id":94438584,"identity":"bbdfa09b-6769-4ad0-8702-42c7c178af6c","added_by":"auto","created_at":"2025-10-27 14:21:35","extension":"png","order_by":19,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":120488,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinefloatimage5.png","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/88d374af67a8caa298a55d30.png"},{"id":94438770,"identity":"5ef2a0c0-8fa3-42de-b126-21186d61200d","added_by":"auto","created_at":"2025-10-27 14:21:50","extension":"png","order_by":20,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":16236,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinegroupimage1.png","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/3a8c0f4638007c7ea8590424.png"},{"id":94489973,"identity":"b69dfb46-a757-4657-809b-de3a40739780","added_by":"auto","created_at":"2025-10-27 17:06:49","extension":"png","order_by":21,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":14445,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinegroupimage2.png","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/a4b9fefd4a9e43db5525d486.png"},{"id":94438741,"identity":"338489d6-83d4-4afb-86b7-43806ea76461","added_by":"auto","created_at":"2025-10-27 14:21:42","extension":"png","order_by":22,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":6769,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinegroupimage3.png","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/b28a33816a78fcf0f48cea02.png"},{"id":94437871,"identity":"7649713a-8d9d-4128-98c1-3add4da20d3d","added_by":"auto","created_at":"2025-10-27 14:21:10","extension":"png","order_by":23,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":24936,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinegroupimage4.png","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/5fe9874d618f74238668a1f1.png"},{"id":94438784,"identity":"a1c1b2d0-243a-4b0a-95ae-98d6c76b989b","added_by":"auto","created_at":"2025-10-27 14:21:56","extension":"png","order_by":24,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":10103,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinegroupimage5.png","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/a67f25aafdf67346d2c873c2.png"},{"id":94437890,"identity":"1cb9872d-da91-4fd4-b066-d6f1154b2f63","added_by":"auto","created_at":"2025-10-27 14:21:11","extension":"png","order_by":25,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":9801,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinegroupimage6.png","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/25c2c446a0b27401ef31f9ef.png"},{"id":94438640,"identity":"610765a4-8bf7-463f-af34-60284ad05b81","added_by":"auto","created_at":"2025-10-27 14:21:38","extension":"png","order_by":26,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":6202,"visible":true,"origin":"","legend":"","description":"","filename":"Onlinegroupimage7.png","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/856a0bb5a6ece9180b2c191c.png"},{"id":94438768,"identity":"c9efa32c-cd82-414c-b6b7-ba054e2b4ea0","added_by":"auto","created_at":"2025-10-27 14:21:50","extension":"xml","order_by":27,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":140402,"visible":true,"origin":"","legend":"","description":"","filename":"b042eb145c364b5093a9f7d80952b5d11structuring.xml","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/c87a77e78b5879273e69a541.xml"},{"id":94438576,"identity":"d68c9247-a735-40fc-a66e-10281852dcca","added_by":"auto","created_at":"2025-10-27 14:21:35","extension":"html","order_by":28,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":148582,"visible":true,"origin":"","legend":"","description":"","filename":"earlyproof.html","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1/361cbb44f956859af1175f9b.html"},{"id":94491338,"identity":"1567f9e9-1b03-4f9c-a5c5-b3cb6c75e4dc","added_by":"auto","created_at":"2025-10-27 17:24:28","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1041406,"visible":true,"origin":"","legend":"","description":"","filename":"Accuracystudyofcolloidalgoldbasedrapidtestreagents.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7696075/v1_covered_38daca54-3eb0-44a7-94e8-4a658172f180.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Accuracy study of colloidal gold-based rapid test reagents for Chinese food","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"food-analytical-methods","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"Learn more about [Food Analytical Methods](https://www.springer.com/journal/12161)","snPcode":"12161","submissionUrl":"https://submission.nature.com/new-submission/12161/3","title":"Food Analytical Methods","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Rapid Test, Chloramphenicol, Enrofloxacin, Sulfonamides, Specificity, Linearity, Sensitivity","lastPublishedDoi":"10.21203/rs.3.rs-7696075/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7696075/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eObjective\u003c/h2\u003e\u003cp\u003eTo evaluate the accuracy of food rapid test reagents based on colloidal gold technology for the detection of chloramphenicol, enrofloxacin and sulfonamides in livestock, poultry and aquatic products, and to provide a reference for their practical application.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e\u003cp\u003eRapid test reagents from different manufacturers were used to detect the above targets in pork, chicken, fish, shrimp, and other samples. The specificity, linearity and sensitivity of the reagents were analysed by setting up a multi-concentration gradient combined with the interference experiment of flavouring matrix.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e\u003cp\u003eReagents D and E showed strong anti-interference ability and specificity for chloramphenicol, though reagent E yielded false positives at 0.05 \u0026micro;g/kg. For enrofloxacin, polynomial fitting correlation coefficients (0.9991, 0.9966, 0.9993) outperformed linear regression. Compared to reagents using sulfadiazine or sulfamethoxazole as the benchmark, those based on sulfamethazine demonstrated superior accuracy. Reagent B achieved an overall accuracy rate of 96.6%. In contrast, reagents using sulfamethoxazole as the benchmark showed no response to sulfadiazine, sulfamerazine, or sulfamethazine, resulting in a false negative rate as high as 100%.\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e\u003cp\u003eThe colloidal gold detection technology is effective in screening for chloramphenicol in seasoned meat and fish samples; for enrofloxacin, it exhibits a good linear relationship and is suitable for preliminary screening in the circulation chain. The sensitivity for sulfonamides depends heavily on the benchmark substance used in reagent design. We recommend selecting rapid tests developed with a structurally representative benchmark, like sulfamethazine, and validating them across matrices before deployment. Confirm with traditional methods if needed.\u003c/p\u003e","manuscriptTitle":"Accuracy study of colloidal gold-based rapid test reagents for Chinese food","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-10-27 10:41:22","doi":"10.21203/rs.3.rs-7696075/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-10-31T14:38:01+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-10-31T12:51:57+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-10-21T10:23:12+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"94616279716629627458655046851806174905","date":"2025-10-17T16:32:40+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-10-16T15:25:38+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"308128790498634444823125334051162776852","date":"2025-10-13T11:04:02+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"96300004576929275894079813044218066185","date":"2025-10-12T16:13:57+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-10-12T16:01:54+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-09-30T09:09:10+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-09-30T09:08:44+00:00","index":"","fulltext":""},{"type":"submitted","content":"Food Analytical Methods","date":"2025-09-23T15:30:25+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"food-analytical-methods","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"Learn more about [Food Analytical Methods](https://www.springer.com/journal/12161)","snPcode":"12161","submissionUrl":"https://submission.nature.com/new-submission/12161/3","title":"Food Analytical Methods","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"2da2dffe-d286-410b-b4a8-54866407fe04","owner":[],"postedDate":"October 27th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[],"tags":[],"updatedAt":"2026-01-17T23:53:15+00:00","versionOfRecord":[],"versionCreatedAt":"2025-10-27 10:41:22","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7696075","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7696075","identity":"rs-7696075","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}