TRIM59 attenuates sleep disturbances and improves cognitive function in PCPA-induced sleep-deprived male mice via the NLRP3 signaling pathway

TRIM59 attenuates sleep disturbances and improves cognitive function in PCPA-induced sleep-deprived male mice via the NLRP3 signaling pathway | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 14 October 2025 V1 Latest version Share on TRIM59 attenuates sleep disturbances and improves cognitive function in PCPA-induced sleep-deprived male mice via the NLRP3 signaling pathway Authors : Yang Zhang 0009-0000-0770-5140 [email protected] , Lin Li , Yilingrui Wang , Xiaoya Hong , and Jing Xu Authors Info & Affiliations https://doi.org/10.22541/au.176042256.61170617/v1 128 views 65 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Purpose: Although Trim59 protects against brain injury, its function and mechanism in sleep disturbances are still unknown. The purpose of this work is to illustrate how Trim59 affects sleep disturbances and the resulting hippocampus damage and cognitive impairment. Methods: PCPA injection was used to create the mouse sleep disorder model, and AAV-Trim59 was stereotaxically injected into the brain to identify Trim59 protein expression in the mouse hippocampal region. Sleep duration and latency were assessed using the pentobarbital sodium sleep experiment, and the hippocampal neurotransmitters 5-HTP, 5-HT, GABA, and Glu were identified using ELISA. The water maze experiment was performed to measure swimming speed, escape delay, number of platform crossings, and target quadrant residence duration. The injury to the hippocampus was examined using H&E staining. The expression of the NLRP3 inflammasome in hippocampal tissue was found using Western blot. Results: Mice who are sleep-deprived exhibit decreased levels of Trim59 expression in their hippocampal regions. Trim59 enhances 5-HTP, 5-HT, and GABA in the hippocampus, decreases Glu content, shortens sleep latency, and extends the duration of sleep in PCPA mice. Additionally, Trim59 lengthens the duration spent in the target quadrant, increases the frequency of platform crossings, and decreases the escape latency of PCPA mice. Trim59 suppresses the expression of the NLRP3 inflammasome protein and lessens hippocampus tissue damage. Conclusion: Trim59 attenuates sleep disorders and enhances learning memory in sleep-deprived mice. Supplementary Material File (ms.docx) Download 96.17 KB Information & Authors Information Version history V1 Version 1 14 October 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Authors Affiliations Yang Zhang 0009-0000-0770-5140 [email protected] The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University View all articles by this author Lin Li The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University View all articles by this author Yilingrui Wang The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University View all articles by this author Xiaoya Hong The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University View all articles by this author Jing Xu The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University View all articles by this author Metrics & Citations Metrics Article Usage 128 views 65 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Yang Zhang, Lin Li, Yilingrui Wang, et al. TRIM59 attenuates sleep disturbances and improves cognitive function in PCPA-induced sleep-deprived male mice via the NLRP3 signaling pathway. Authorea . 14 October 2025. DOI: https://doi.org/10.22541/au.176042256.61170617/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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