Antibacterial and seed germination activities of biosynthesized zinc oxide nanoparticles using aqueous leaf and fruit extract of Annona reticulata

Antibacterial and seed germination activities of biosynthesized zinc oxide nanoparticles using aqueous leaf and fruit extract of Annona reticulata | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Antibacterial and seed germination activities of biosynthesized zinc oxide nanoparticles using aqueous leaf and fruit extract of Annona reticulata C. Shobana, J. P. Malavika, V. D. Nithya, M. Vishwanathan, P. Srinithish, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4263405/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 3 You are reading this latest preprint version Abstract In the present work, the zinc oxide nanoparticles were synthesized using an environmentally friendly green synthesis method utilizing Annona reticulata leaf and fruit extracts and compared with the ZnO prepared by the chemical route method. The Rietveld refinement X-ray diffraction (XRD) pattern revealed the formation of wurtzite-structured ZnO. The presence of abundant hydroxyl functional groups with Zn-O stretching vibrations was corroborated using Fourier Transform Infrared (FTIR) spectroscopic analysis. The particle size distribution and the morphological analysis were inferred through the Field emission scanning electron microscopic (FESEM) images. The antibacterial potential of the prepared ZnO NPs was evaluated against pathogenic strains of Staphylococcus aureus , Enterococcus faecalis , Klebsiella pneumoniae , Pseudomonas aeruginosa , and Escherichia coli . Based on the zone of inhibition, the fruit-derived ZnO NPs showed higher antibacterial activity, followed by leaf-derived and chemically synthesized nanoparticles. Notably, fruit-derived ZnO NPs exhibited an almost equal zone of inhibition of 21.1 mm at 100 µg and a greater zone of inhibition of 16.9 mm at 50 µg against Klebsiella pneumoniae compared to antibiotic streptomycin. Finally, the germination of Vigna radiata seeds under the influence of ZnO NPs demonstrated that the nanoparticles favoured seed germination at a lower concentration of 2.5 mg. The highest seed germination was observed in green synthesized ZnO NPs (26 cm), with both leaf and fruit-derived nanoparticles exhibiting almost similar impacts on seed germination. The findings indicate that Annona reticulata-derived ZnO NPs could be utilized as potential antibacterial agents. Zinc Oxide Annona reticulata Antibacterial Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Editor assigned by journal 17 Apr, 2024 Submission checks completed at journal 16 Apr, 2024 First submitted to journal 13 Apr, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4263405","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":292306132,"identity":"1e722867-527e-4d88-ab22-e067b58d508d","order_by":0,"name":"C. 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The Rietveld refinement X-ray diffraction (XRD) pattern revealed the formation of wurtzite-structured ZnO. The presence of abundant hydroxyl functional groups with Zn-O stretching vibrations was corroborated using Fourier Transform Infrared (FTIR) spectroscopic analysis. The particle size distribution and the morphological analysis were inferred through the Field emission scanning electron microscopic (FESEM) images. The antibacterial potential of the prepared ZnO NPs was evaluated against pathogenic strains of \u003cem\u003eStaphylococcus aureus\u003c/em\u003e, \u003cem\u003eEnterococcus faecalis\u003c/em\u003e, \u003cem\u003eKlebsiella pneumoniae\u003c/em\u003e, \u003cem\u003ePseudomonas aeruginosa\u003c/em\u003e, and \u003cem\u003eEscherichia coli\u003c/em\u003e. Based on the zone of inhibition, the fruit-derived ZnO NPs showed higher antibacterial activity, followed by leaf-derived and chemically synthesized nanoparticles. Notably, fruit-derived ZnO NPs exhibited an almost equal zone of inhibition of 21.1 mm at 100 \u0026micro;g and a greater zone of inhibition of 16.9 mm at 50 \u0026micro;g against \u003cem\u003eKlebsiella pneumoniae\u003c/em\u003e compared to antibiotic streptomycin. Finally, the germination of \u003cem\u003eVigna radiata\u003c/em\u003e seeds under the influence of ZnO NPs demonstrated that the nanoparticles favoured seed germination at a lower concentration of 2.5 mg. The highest seed germination was observed in green synthesized ZnO NPs (26 cm), with both leaf and fruit-derived nanoparticles exhibiting almost similar impacts on seed germination. The findings indicate that \u003cem\u003eAnnona reticulata-derived\u003c/em\u003e ZnO NPs could be utilized as potential antibacterial agents.\u003c/p\u003e","manuscriptTitle":"Antibacterial and seed germination activities of biosynthesized zinc oxide nanoparticles using aqueous leaf and fruit extract of Annona reticulata","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-04-19 03:24:30","doi":"10.21203/rs.3.rs-4263405/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"editorAssigned","content":"","date":"2024-04-17T11:12:50+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-04-17T01:44:59+00:00","index":"","fulltext":""},{"type":"submitted","content":"BioNanoScience","date":"2024-04-14T02:31:45+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bionanoscience","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bnsc","sideBox":"Learn more about [BioNanoScience](http://link.springer.com/journal/12668)","snPcode":"12668","submissionUrl":"https://submission.nature.com/new-submission/12668/3","title":"BioNanoScience","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"849d49bc-a1ac-41cf-9cc7-64eb6e719650","owner":[],"postedDate":"April 19th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[],"tags":[],"updatedAt":"2024-04-19T03:24:30+00:00","versionOfRecord":[],"versionCreatedAt":"2024-04-19 03:24:30","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4263405","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4263405","identity":"rs-4263405","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}