Tech Note: Increased Complexity of Amplicon Libraries using Phased Primers

Abstract Phased primers are used during the first PCR of amplicon library preparation in order to increase the complexity of the library. This increased complexity leads to improved sequencing quality even when sequencing with a 2×300 paired-end setup. We can show that libraries prepared using a phased primer approach are complex enough to reduce the amount of PhiX added to the sequencing reaction to that of other, complex libraries. Competing Interest Statement The authors have declared no competing interest. Footnotes Phased primers are used during the first PCR of amplicon library preparation in order to increase the complexity of the library. This increased complexity leads to improved sequencing quality. Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.