Effect of Triptolide on Cytotoxicity and NO production of Activated Mouse Peritoneal Macrophage

Objective:To study the mechanism of immune inhibition of triptolide(TP) on activated peritoneal macrophage(PEMΦ)in humane endometriosis and the effect of different dosage of TP on Cytotoxicity and NO production in different period.Methods:The activated PEMΦ induced by LPS was cultured in vitro.The cytotoxicity of MΦ was tested by MTT colorimetry and the level of NO by Griess reagent.Results:TP with the dosage of 1~10 μg/ml could significantly inhibit the cytotoxicity (P0.0 1)and NO production of PEMΦ and had no cytotoxicity to PEMΦ.TP with the dosage less than 1μg/ml had no statistic meaning.Conclusion:TP can efficiently inhibit the cytotoxicity and NO production of activated PEMΦ,which provides experimental proof for the clinical treatment of endometriosis.