Single-cell atlas reveals the resistance of cortex cells to bacterial wilt in peanut roots

Single-cell atlas reveals the resistance of cortex cells to bacterial wilt in peanut roots | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Single-cell atlas reveals the resistance of cortex cells to bacterial wilt in peanut roots dongmei yin, Kai Zhao, Yanzhe Li, Zhuo Chen, Zenghui Cao, Xingli Ma, and 13 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6003167/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Peanut bacterial wilt (BW) is an extremely destructive soil-borne disease capable of causing yield losses exceeding 50%. However, the molecular mechanism underlying resistance to BW remains elusive. Here, we present the first single-cell expression atlas of peanut roots, comparing a BW-resistant ariety (H108) with a susceptible variety (H107). We profiled 24,384 and 23,693 cells from the varieties, categorizing them into 10 distinct cell types with in situ hybridization confirming. Among them, 2,148 differentially expressed genes (DEGs) were predominantly concentrated in cortex cells. Notably, the Ah2J79JM gene, which encodes protease inhibitors (AhKTI), was validated as responsive to Ralstonia solanacearum infection. Overexpression of Ah2J79JM in peanuts resulted in more densely arranged cortex cells, significantly enhancing resistance to BW. The study highlights the architecture of cortex cells as a crucial defensive barrier and provides a valuable single-cell genetic resource, along with insights into the mechanisms underlying BW resistance to facilitate peanut improvement. Biological sciences/Genetics/Agricultural genetics Biological sciences/Cell biology Peanut bacterial wilt single cell atlas cortex cells trypsin inhibitor Full Text Additional Declarations There is NO Competing Interest. Supplementary Files SupplementaryTables16.rar Supplementary Tables. 1-6 SupplementaryFigures.17.docx Supplementary Figures and legends 1-7 Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6003167","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":419998880,"identity":"80e6b849-8e5a-422b-9e81-b8bd21583c00","order_by":0,"name":"dongmei 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