PCR Instrument-assisted Acidolysis for Monosaccharide Composition Analysis of Serum Glycans
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Abstract
Abstract This protocol describes the procedures where a PCR instrument-assisted acidolysis is used for releasing monosaccharides from serum glycans. The monosaccharide composition analysis is subsequently obtained by a HPLC method that separates and quantifies all 1-phenyl-3-methyl-5-pyrazolone (PMP)-labeled monosaccharides in 10 μl serum in 20 minutes. The rapid heating, precise temperature control, and gradient heating properties of PCR instrument provides with consistent acidolysis and derivatization conditions for up to 96 samples simultaneously. The described workflow takes approximately 4–5 h, up to 72 serum samples can be analyzed with one HPLC instrument per day.
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- last seen: 2026-05-19T01:45:01.086888+00:00