Investigating genetic spillage in a Mimulus cardinalis (syn. Erythranthe cardinalis) common garden experiment

Investigating genetic spillage in a Mimulus cardinalis (syn. Erythranthe cardinalis) common garden experiment | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 27 March 2026 V1 Latest version Share on Investigating genetic spillage in a Mimulus cardinalis (syn. Erythranthe cardinalis) common garden experiment Authors : Lydia Duran , Niveditha Ramadoss 0000-0002-3647-3761 , Seema Sheth 0000-0001-8284-7608 , and Lluvia Flores-Rentería 0000-0001-8934-0033 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.177459339.99905288/v1 152 views 98 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Background and Aims Common gardens are critical to studying the trait variations in plant species and how environmental or genetic factors influence them. In common gardens, non-native species and non-local populations must be monitored closely to reduce their chances of escaping, as it can lead to genetic spillage, which is the introduction of foreign genetic material with potential unwanted phenotypes/genotypes into the native ecosystems. This could decrease the fitness of native populations and threaten natural habitats. This study investigated a possible spillage of genetic material from an existing common garden experiment of the Scarlet Monkeyflower. This garden consists of six populations that originated from three regions across its distribution (North, Central, and South), and were propagated at an Ecological Reserve in Southern California. A population of E. cardinalis was spotted a kilometer away from the common garden, leading to the question of whether it is a wild population or an escapee. Methods Leaf samples were collected from individuals of each provenance established at the common garden, as well as the “unknown” population. DNA was extracted from all samples and further processed for DArT sequencing to generate genome-wide SNPs. Genetic clustering analyses such as DAPC, PCoA, and ADMIXTURE were used to determine the genetic proximity of the unknown population against the six provenances from the common garden. Key Results Clustering analyses of all individuals revealed that the unknown population is genetically related to southern populations. A focused analysis of only the southern and unknown populations showed that the unknown individuals formed a distinct cluster, suggesting they are not escapees but represent a natural population in that region. Conclusion Although a spillage was not detected, these findings serve as a reminder for researchers to monitor their garden experiments to control escapees and reduce the possibility of a spillage from occurring. Supplementary Material File (erythranthe manuscript eande.docx) Download 5.89 MB Information & Authors Information Version history V1 Version 1 27 March 2026 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords comparative ecological experiment evolutionary ecology plants terrestrial Authors Affiliations Lydia Duran San Diego State University View all articles by this author Niveditha Ramadoss 0000-0002-3647-3761 San Diego State University College of Sciences View all articles by this author Seema Sheth 0000-0001-8284-7608 North Carolina State University View all articles by this author Lluvia Flores-Rentería 0000-0001-8934-0033 [email protected] San Diego State University View all articles by this author Metrics & Citations Metrics Article Usage 152 views 98 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Lydia Duran, Niveditha Ramadoss, Seema Sheth, et al. Investigating genetic spillage in a Mimulus cardinalis (syn. Erythranthe cardinalis) common garden experiment. Authorea . 27 March 2026. DOI: https://doi.org/10.22541/au.177459339.99905288/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! 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