The Effects of Dolutegravir-Art on Mitochondrial Function and Iron Homeostasis in HIV Patients

The Effects of Dolutegravir-Art on Mitochondrial Function and Iron Homeostasis in HIV Patients | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article The Effects of Dolutegravir-Art on Mitochondrial Function and Iron Homeostasis in HIV Patients ONWUKA KALU CHIMA OKPO, EJIKE FELIX CHUKWURAH This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6876482/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Dolutegravir-based antiretroviral therapy is the recommended standard treatment plan in many countries globally due to its comparative advantage over previous drugs for managing HIV/AIDS, although safety concerns have been documented. This research assessed iron levels and the mitochondrial dysfunction biomarker, cytochrome c, in patients on dolutegravir-based antiretroviral therapy at University of Nigeria Teaching Hospital, UNTH,Ituku-Ozalla, Enugu. A total of 40 treatment-naïve HIV patients with no co morbidities were prospectively recruited for the study, along with apparently healthy, sex- and age-matched individuals as negative controls. Baseline and follow-up specimens were collected and analyzed for cytochrome c using specific immunoenzymatic methods and for total iron using the iron Nitro PAPS chromogen spectrophotometric assay, respectively. The mean pre-treatment values for cytochrome c and iron were 30.91 ± 13.97 ng/ml and 14.54 ± 4.56 µmol/l, respectively. After 6 months of DTG-based ART, the mean values of cytochrome c and total iron increased significantly to 43.31 ± 23.29 ng/ml and 23.54 ± 4.99 µmol/l, respectively. These findings suggest possible iron overload and mitochondrial toxicity effects of DTG-based ART on iron status and mitochondrial health. Cellular Metabolism DOLUTEGRAVIR MITOCHONDRIA IRON HAEMOSTASIS FUNCTION Full Text Additional Declarations Ethics Approval and Consent to Participate This study was approved by the University of Nigeria Teaching Hospital Health Research Ethics Committee, with approval number NHREC/05/01/2008B-FWA00002458-IRB00002323 Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6876482","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":470130696,"identity":"f2ae3b0d-110f-42a8-a943-735f9131af0c","order_by":0,"name":"ONWUKA KALU CHIMA OKPO","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA+klEQVRIiWNgGAWjYJACxgaGBAY+BgY2IMUgBxI58IAYLWxQLcZgLQlEawGCxAYQiU8Lv3SPmeSMmjR5NonkZw8e5tilzw87/BBoi52cbgN2LZJzzphJbjiWY9gmkWZukLgtOXfj7TQDoJZkY7MD2LUY3MjdJvmArYKxTSKHTSJxG3PuxtkJIC0HErfh1fKvwh6qpT7dcHb6B8JaNrblJEK1HE6Ql87Bb4vkjPzPljP70pLbeJ6ZAbUcN9wgnVNwIMEAt1/4JdISb/Z8S7btZ09+JvlzW7W8/Oz0zR8+VNjJ4dKCxalglQbEKgcB+QZSVI+CUTAKRsFIAACK8WFtSvOkQQAAAABJRU5ErkJggg==","orcid":"https://orcid.org/0009-0001-9882-5494","institution":"EBONYI STATE UNIVERSITY ABAKALIKI, EBONYI STATE","correspondingAuthor":true,"prefix":"","firstName":"ONWUKA","middleName":"KALU CHIMA","lastName":"OKPO","suffix":""},{"id":470131100,"identity":"5801ab27-af78-41fc-a148-02274d6e1b3a","order_by":1,"name":"EJIKE FELIX CHUKWURAH","email":"","orcid":"","institution":"EBONYI STATE UNIVERSITY ,ABAKALIKI.EBONYI STATE","correspondingAuthor":false,"prefix":"","firstName":"EJIKE","middleName":"FELIX","lastName":"CHUKWURAH","suffix":""}],"badges":[],"createdAt":"2025-06-12 04:52:30","currentVersionCode":1,"declarations":{"humanSubjects":true,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":true,"humanSubjectConsent":true,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-6876482/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6876482/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":84543598,"identity":"795aa4bd-dbe2-41bc-8e63-dead5bfaa05d","added_by":"auto","created_at":"2025-06-13 08:54:59","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":283144,"visible":true,"origin":"","legend":"","description":"","filename":"EFFECTOFDTGONIRONHAEMOSTAISIS.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6876482/v1_covered_686e7519-6d10-40ca-9f8e-2eca2ebc3c37.pdf"}],"financialInterests":"\u003cp\u003eEthics Approval and Consent to Participate This study was approved by the University of Nigeria Teaching Hospital Health Research Ethics Committee, with approval number NHREC/05/01/2008B-FWA00002458-IRB00002323\u003c/p\u003e","formattedTitle":"\u003cp\u003eThe Effects of Dolutegravir-Art on Mitochondrial Function and Iron Homeostasis in HIV Patients\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"Ebonyi State University","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"DOLUTEGRAVIR, MITOCHONDRIA, IRON, HAEMOSTASIS, FUNCTION","lastPublishedDoi":"10.21203/rs.3.rs-6876482/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6876482/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eDolutegravir-based antiretroviral therapy is the recommended standard treatment plan in many countries globally due to its comparative advantage over previous drugs for managing HIV/AIDS, although safety concerns have been documented. 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