Alkaline pretreatment of food waste with grass clippings for the enhancement of methane production in anaerobic digestion processes

Alkaline pretreatment of food waste with grass clippings for the enhancement of methane production in anaerobic digestion processes | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Alkaline pretreatment of food waste with grass clippings for the enhancement of methane production in anaerobic digestion processes MATHEUS VITOR DINIZ GUERI, William Gouvêa Buratto, Ademir Nied, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4607875/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The proper management of solid urban waste is essential to avoid the contamination of natural resources and greenhouse gas emissions. In Brazil, approximately 50% of municipal solid waste is made up of food and garden waste. Anaerobic co-digestion can convert this waste into energy and biofertilizer, but hydrolysis of the lignocellulosic components is a barrier and pre-treatment is necessary. This study sought to identify the biochemical methane potential of the co-digestion of food waste with garden waste (grass clippings) subjected to alkaline hydrolysis. 3% sodium hydroxide (NaOH) (wet state) was added to the grass clippings for 12, 24 and 48 hours. The biochemical methane potential tests followed the recommendations of the German standard VDI 4630 (2006) for 31 days at a mesophilic temperature (37 ºC ± 1). Total and volatile solids, chemical oxygen demand, total Kjeldahl nitrogen, total organic carbon, carbon/nitrogen ratio, carbohydrates, cellulose, hemicellulose, and lignin were analyzed. The samples followed a ratio of ≤ 0.5 with the inoculum and 1:1 for substrate and cosubstrate (on VS basis). The results showed that the co-digestion of food waste with grass clippings was advantageous due to the balance of nutrients, raising the carbon/nitrogen ratio 21 to 25. The greatest generation of biogas and methane was seen in the co-digestion 12h sample, which showed the greatest reduction in solids. Alkaline hydrolysis was efficient, reducing the lignin content by 40.95% and increasing the substrate conversion and maximum production rates by an average of 1.25 and 1.05 times. The improvement in the digestibility of food waste co-digestion samples with NaOH pre-treated grass clippings was evident in all configurations. It was possible to conclude that grass clippings subjected to pre-treatment with 3% of NaOH for 12 hours are recommended as a co-substrate with food waste, due to the improvement observed in methane generation. Anaerobic digestion processes food waste methane production Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4607875","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":322439471,"identity":"d169b743-9ecf-4153-a535-ad154bf34b1c","order_by":0,"name":"MATHEUS VITOR DINIZ 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In Brazil, approximately 50% of municipal solid waste is made up of food and garden waste. Anaerobic co-digestion can convert this waste into energy and biofertilizer, but hydrolysis of the lignocellulosic components is a barrier and pre-treatment is necessary. This study sought to identify the biochemical methane potential of the co-digestion of food waste with garden waste (grass clippings) subjected to alkaline hydrolysis. 3% sodium hydroxide (NaOH) (wet state) was added to the grass clippings for 12, 24 and 48 hours. The biochemical methane potential tests followed the recommendations of the German standard VDI 4630 (2006) for 31 days at a mesophilic temperature (37 ºC ± 1). Total and volatile solids, chemical oxygen demand, total Kjeldahl nitrogen, total organic carbon, carbon/nitrogen ratio, carbohydrates, cellulose, hemicellulose, and lignin were analyzed. The samples followed a ratio of ≤ 0.5 with the inoculum and 1:1 for substrate and cosubstrate (on VS basis). The results showed that the co-digestion of food waste with grass clippings was advantageous due to the balance of nutrients, raising the carbon/nitrogen ratio 21 to 25. The greatest generation of biogas and methane was seen in the co-digestion 12h sample, which showed the greatest reduction in solids. Alkaline hydrolysis was efficient, reducing the lignin content by 40.95% and increasing the substrate conversion and\nmaximum production rates by an average of 1.25 and 1.05 times. The improvement in the digestibility of food waste co-digestion samples with NaOH pre-treated grass clippings was evident in all configurations. 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