Untargeted proteomics highlights interaction between barrier dysfunction and immune dysregulation as key pathways in Atopic Dermatitis in Children

Untargeted proteomics highlights interaction between barrier dysfunction and immune dysregulation as key pathways in Atopic Dermatitis in Children | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Pediatric Allergy and Immunology This is a preprint and has not been peer reviewed. Data may be preliminary. 20 February 2025 V1 Latest version Share on Untargeted proteomics highlights interaction between barrier dysfunction and immune dysregulation as key pathways in Atopic Dermatitis in Children Authors : Sean Wee 0000-0001-6486-5669 , Anna de Beer 0000-0002-3101-2835 , Hiromi Koh 0000-0002-6894-5129 , Jocelyn Ong , Jing Hui Low , Yan Ting Lim , Shi Mei Wang , … Show All … , Radoslaw Sobota M , Elena Goleva , Donald Leung 0000-0002-0177-3844 , Wen Chin Chiang , Mei Chien Chua , and Anand Andiappan 0000-0002-8442-1544 [email protected] Show Fewer Authors Info & Affiliations https://doi.org/10.22541/au.174004045.53559769/v1 Published Pediatric Allergy and Immunology Version of record Peer review timeline 465 views 262 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Background: Atopic Dermatitis (AD) is a chronic condition characterised by recurrent skin lesions and itch, significantly impacting patients’ quality of life. AD is most common during childhood, with global prevalence ranging between 10% and 20%. Objective: Identifying protein biomarkers underlying AD in children, to better understand the pathogenesis. Methods: Cross-sectional study using untargeted tandem mass spectrometry to analyse skin tape protein extracts from 71 10-year-old Singaporean children. Subjects were stratified into three groups, no history of AD (NoAD=29), history of AD but no symptoms at time of sample collection (PastAD=22) and present AD (PresentAD=20). Significant proteins were shortlisted and functional analyses were performed. Results: 397 differentially expressed proteins were identified (p-value<0.05) with 18 proteins shared with a validation cohort. Ingenuity pathway analysis shows that ‘PastAD vs PresentAD’ share common pathways with ‘NoAD vs PresentAD’. The top pathways obtained are related to inflammatory responses, such as neutrophil degranulation, JAK-STAT signalling and interleukin-1 family signalling. Conclusions: Multiple protein biomarkers and pathways, including keratinization, inflammatory responses and oxidative stress response, are involved in the onset and persistence of AD. Despite resolution of disease manifestations in PastAD subjects, functional analyses show that some pathways and biomarkers indicative of AD persist. Supplementary Material File (manuscript.docx) Download 62.01 KB File (table 1.docx) Download 17.39 KB File (table2.docx) Download 18.15 KB Information & Authors Information Version history V1 Version 1 20 February 2025 Peer review timeline Published Pediatric Allergy and Immunology Version of Record 4 Jul 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection Pediatric Allergy and Immunology Authors Affiliations Sean Wee 0000-0001-6486-5669 Singapore Immunology Network View all articles by this author Anna de Beer 0000-0002-3101-2835 Singapore Immunology Network View all articles by this author Hiromi Koh 0000-0002-6894-5129 Institute of Molecular and Cell Biology View all articles by this author Jocelyn Ong Singapore Immunology Network View all articles by this author Jing Hui Low Singapore Immunology Network View all articles by this author Yan Ting Lim Institute of Molecular and Cell Biology View all articles by this author Shi Mei Wang Institute of Molecular and Cell Biology View all articles by this author Radoslaw Sobota M Institute of Molecular and Cell Biology View all articles by this author Elena Goleva National Jewish Health South Denver View all articles by this author Donald Leung 0000-0002-0177-3844 National Jewish Health South Denver View all articles by this author Wen Chin Chiang KK Women's and Children's Hospital Children's Services View all articles by this author Mei Chien Chua KK Women's and Children's Hospital View all articles by this author Anand Andiappan 0000-0002-8442-1544 [email protected] Singapore Immunology Network View all articles by this author Metrics & Citations Metrics Article Usage 465 views 262 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Sean Wee, Anna de Beer, Hiromi Koh, et al. Untargeted proteomics highlights interaction between barrier dysfunction and immune dysregulation as key pathways in Atopic Dermatitis in Children. Authorea . 20 February 2025. DOI: https://doi.org/10.22541/au.174004045.53559769/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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