Alpha-1-antitrypsin and Regulatory T cell cytokines in lungs of Cystic fibrosis patients

Alpha-1-antitrypsin and Regulatory T cell cytokines in lungs of Cystic fibrosis patients | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 15 January 2025 V1 Latest version Share on Alpha-1-antitrypsin and Regulatory T cell cytokines in lungs of Cystic fibrosis patients Authors : Kerstin Michael [email protected] , Sophie Mandelburger , Klara Schmidthaler , Anna Stadlbauer , Wolfgang Hübl , Matthias Reinweber , Thomas Frischer , and Angela Zacharasiewicz Authors Info & Affiliations https://doi.org/10.22541/au.173697422.20317896/v1 253 views 141 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Background: Cystic fibrosis (CF) is a pulmonary disease leading to the destruction of lung tissue caused by repeated infection with typical pathogens like Pseudomonas aeruginosa (PA). Different T cell subsets, an imbalance in cytokine levels and a protease-antiprotease imbalance play a role. This study aimed to investigate the potential influence of alpha-1-antitrypsin (AAT) on various cytokines in CF. Methods: Levels of AAT and T-helper-cell-associated cytokines (GM-CSF, IL2, IL6, IL10, IL12p70, IL13, IL17, IL21, IL23, TGFβ,) have been measured in supernatant of induced sputum of CF patients and compared between patients who tested positive for PA (PA-positive) and those who tested negative (PA-negative). Results: Measurements have been performed for 17 CF patients (mean age 14.16 (±4.22) years, 9 female/8 male, 9 PA-positive, 8 PA-negative). AAT correlates with Treg-associated cytokines IL10 and IL12p70 (p < 0.05 and p < 0.05 respectively), a borderline significance with TGFβ exists (p = 0.06). AAT and Treg-associated cytokines IL10, IL12p70 and TGFβ are lower in PA-positive CF patients compared to PA-negative CF patients (24.78 vs. 41.1 pg/ml, p = 0.69, 0.14 vs. 0.26 ng/ml, p = 0.68, 0.17 vs. 0.41 ng/ml, p = 0.32 and 109.32 vs. 227.96 ng/ml, p = 0.85, respectively). GM-CSF significantly correlates with AAT (r=0.75; p<0.05) and Treg-associated cytokines IL2, IL10, IL12p70, IL13 and TGFβ (r=0.93; p<0.001, r=0.9; p<0.01, r=0.73; p<0.05, r=0.97; p<0.001 and r=0.88; p<0.01, respectively). Conclusion: In this cross-sectional study AAT correlates with Treg-associated cytokines. Higher levels of AAT are observed in PA-negative CF patients. Supplementary Material File (michael et al_2.docx) Download 100.39 KB File (table 1_jpp.docx) Download 74.66 KB File (table 2_jpp.docx) Download 70.73 KB File (table 3_jpp.docx) Download 68.44 KB Information & Authors Information Version history V1 Version 1 15 January 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords alpha-1-antitrypsin cystic fibrosis lung regulatory t cells Authors Affiliations Kerstin Michael [email protected] Wiener Gesundheitsverbund Klinik Ottakring View all articles by this author Sophie Mandelburger Wiener Gesundheitsverbund Klinik Ottakring View all articles by this author Klara Schmidthaler Medizinische Universitat Wien View all articles by this author Anna Stadlbauer Wiener Gesundheitsverbund Klinik Ottakring View all articles by this author Wolfgang Hübl Wiener Gesundheitsverbund Klinik Ottakring View all articles by this author Matthias Reinweber Wiener Gesundheitsverbund View all articles by this author Thomas Frischer Wiener Gesundheitsverbund Klinik Ottakring View all articles by this author Angela Zacharasiewicz Wiener Gesundheitsverbund Klinik Ottakring View all articles by this author Metrics & Citations Metrics Article Usage 253 views 141 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Kerstin Michael, Sophie Mandelburger, Klara Schmidthaler, et al. Alpha-1-antitrypsin and Regulatory T cell cytokines in lungs of Cystic fibrosis patients. Authorea . 15 January 2025. DOI: https://doi.org/10.22541/au.173697422.20317896/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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