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The study developed an ELISA to detect human anti-ovarian autoantibodies using bovine corpus luteum extracts fractionated into soluble and membrane components, as well as an isolated luteinizing hormone/human chorionic gonadotropin receptor. Sera from patients with primary or secondary sterility and endometriosis were tested, with positive IgG autoantibody reactions detected in subsets across endometriosis-associated sterility and in vitro fertilization cohorts; antibody recognition varied between the unoccupied LH/hCG receptor and the hormone–receptor complex. Antibody subclasses differed by antigen fraction, and glycosylation analyses indicated main terminal glycosides, while cross-reactivity experiments supported antigenic relatedness among fractions. A stated limitation is that the work used bovine corpus luteum and receptor antigens to infer human anti-ovarian specificity. This paper is centrally about endometriosis — it reports anti-ovarian antibody detection specifically in patients with sterility and endometriosis, using ovarian and LH/hCG receptor targets.
Abstract
We developed an ELISA system for the detection of human anti-ovarian antibodies. Bovine corpora lutea were extracted in PBS (pH 7.2) and fractionated by ultracentrifugation. Both the soluble fraction obtained after 80,000 g (S80) and the Triton-extracted membrane fraction (ST288) were used as antigens. Additionally, the luteinizing hormone (LH)/human chorionic gonadotropin (hCG) receptor was isolated by affinity chromatography (wheat germ agglutinin and LH-Sepharose) and also used as an antigen. In 7 of 14 patients with primary sterility and endometriosis a positive reaction was observed. Similarly, 6 of 16 patients with secondary sterility and endometriosis were also positive. Patients being stimulated for in vitro fertilization and presenting either primary or secondary sterility were positive in 5 of 22 and 6 of 16 cases, respectively. In the S80 test 41 of 60 sera presented IgG2 antibodies, whereas in the ST288 test 38 of 60 belonged to the IgG1 subclass. Kappa and lambda chains were equally distributed. Some patients could recognize the unoccupied LH/hCG receptor as an antigen, while others recognized only the complex formed by the hormone plus the hormone receptor. The S80 and ST288 antigens were isolated by affinity chromatography. Gel permeation of the purified antigens revealed in each case the presence of an antigen complex. The apparent molecular weight was between 2,000 and 36,000 D. Cross-reactivity studies using affinity-purified antibodies demonstrated an antigenic relationship of the membrane, soluble, and extractable fractions. NAc-(beta-1----4)-D-glucosaminide and -D-galactopyranoside were the main terminal glycosides.
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Research Article Free access | 10.1172/JCI114372
Department of Obstetrics and Gynecology, University of Ulm, Federal Republic of Germany.
Find articles by Moncayo, H. in: PubMed | Google Scholar
Department of Obstetrics and Gynecology, University of Ulm, Federal Republic of Germany.
Find articles by Moncayo, R. in: PubMed | Google Scholar
Department of Obstetrics and Gynecology, University of Ulm, Federal Republic of Germany.
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Department of Obstetrics and Gynecology, University of Ulm, Federal Republic of Germany.
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Department of Obstetrics and Gynecology, University of Ulm, Federal Republic of Germany.
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Published December 1, 1989 - More info
Published in
Volume 84, Issue 6
on
December 1, 1989
J Clin Invest. 1989;84(6):1857–1865. https://doi.org/10.1172/JCI114372.
© 1989 The American Society for Clinical Investigation
J Clin Invest. 1989;84(6):1857–1865. https://doi.org/10.1172/JCI114372.
© 1989 The American Society for Clinical Investigation
Published December 1, 1989
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Abstract
We developed an ELISA system for the detection of human anti-ovarian antibodies. Bovine corpora lutea were extracted in PBS (pH 7.2) and fractionated by ultracentrifugation. Both the soluble fraction obtained after 80,000 g (S80) and the Triton-extracted membrane fraction (ST288) were used as antigens. Additionally, the luteinizing hormone (LH)/human chorionic gonadotropin (hCG) receptor was isolated by affinity chromatography (wheat germ agglutinin and LH-Sepharose) and also used as an antigen. In 7 of 14 patients with primary sterility and endometriosis a positive reaction was observed. Similarly, 6 of 16 patients with secondary sterility and endometriosis were also positive. Patients being stimulated for in vitro fertilization and presenting either primary or secondary sterility were positive in 5 of 22 and 6 of 16 cases, respectively. In the S80 test 41 of 60 sera presented IgG2 antibodies, whereas in the ST288 test 38 of 60 belonged to the IgG1 subclass. Kappa and lambda chains were equally distributed. Some patients could recognize the unoccupied LH/hCG receptor as an antigen, while others recognized only the complex formed by the hormone plus the hormone receptor. The S80 and ST288 antigens were isolated by affinity chromatography. Gel permeation of the purified antigens revealed in each case the presence of an antigen complex. The apparent molecular weight was between 2,000 and 36,000 D. Cross-reactivity studies using affinity-purified antibodies demonstrated an antigenic relationship of the membrane, soluble, and extractable fractions. NAc-(beta-1----4)-D-glucosaminide and -D-galactopyranoside were the main terminal glycosides.
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Version history
- Version 1 (December 1, 1989): No description
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ISSN: 0021-9738 (print), 1558-8238 (online)
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