Solid-phase chemiluminescence immunoassay for progesterone in unextracted serum.

J. De Boever; Förtüne Kohen; D Vandekerckhove; Georges Van Maele

Solid-phase chemiluminescence immunoassay for progesterone in unextracted serum.

J. De Boever, Förtüne Kohen, D Vandekerckhove, Georges Van Maele

In: Clinical chemistry · 1984 · vol. 30(10) , pp. 1637–41 · PMID:6383657 · W1828449755

article OA: closed CC0 ⤵ 1 in-corpus citation

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A direct chemiluminescence immunoassay for progesterone in unextracted serum offers similar sensitivity, specificity, precision, and accuracy to conventional radioimmunoassays, providing a convenient alternative.

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Abstract

We describe a simple, solid-phase chemiluminescence immunoassay for progesterone in 10 microL of unextracted serum ("direct" assay). Danazol at pH 8.0 is included (100 ng per tube) to displace progesterone from binding proteins in serum. A progesterone-11 alpha-hemisuccinyl-aminobutylethyl isoluminol conjugate serves as the chemiluminescent ligand marker and homologous antiprogesterone IgG covalently coupled to "Immunobeads" is the immunoadsorbant. After the binding reaction, bound and free ligand are separated by centrifugation and the chemiluminescence yield of the bound label is determined. The sensitivity, specificity, precision, and accuracy of the method are similar to those of a conventional radioimmunoassay for progesterone in which a radioligand of tritiated progesterone and serum extraction are used. Progesterone values obtained by this procedure agreed well (r = 0.987) with those obtained by radioimmunoassay. We conclude that the chemiluminescence immunoassay for progesterone in unextracted serum is analytically valid and offers a convenient alternative to radioimmunoassay.

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Cited by (1)

Poor Endometrial Proliferation After Clomiphene is Associated With Altered Estrogen Action 2021

References (9)

doi:10.1093/clinchem/26.11.1607 via openalex
doi:10.1016/0039-128x(80)90029-x via openalex
doi:10.1016/0009-8981(81)90242-4 via openalex
doi:10.1016/0009-8981(81)90241-2 via openalex
doi:10.1530/acta.0.065s320 via openalex
doi:10.1210/jcem-53-1-58 via openalex
doi:10.1016/0022-4731(83)90196-6 via openalex
doi:10.1016/s0039-128x(83)90156-3 via openalex
doi:10.1093/clinchem/29.12.2068 via openalex

Cited by (1)

Poor Endometrial Proliferation After Clomiphene is Associated With Altered Estrogen Action 2021

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[1] doi:10.1093/clinchem/26.11.1607 via openalex

[2] doi:10.1016/0039-128x(80)90029-x via openalex

[3] doi:10.1016/0009-8981(81)90242-4 via openalex

[4] doi:10.1016/0009-8981(81)90241-2 via openalex

[5] doi:10.1530/acta.0.065s320 via openalex

[6] doi:10.1210/jcem-53-1-58 via openalex

[7] doi:10.1016/0022-4731(83)90196-6 via openalex

[8] doi:10.1016/s0039-128x(83)90156-3 via openalex

[9] doi:10.1093/clinchem/29.12.2068 via openalex