Biogenic Synthesis of Intracellular Silver Nanoparticle by Endophytic Ascomycetes Fungi From Calotropis gigantea Seeds (L.) R. Br

Biogenic Synthesis of Intracellular Silver Nanoparticle by Endophytic Ascomycetes Fungi From Calotropis gigantea Seeds (L.) R. Br | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Biogenic Synthesis of Intracellular Silver Nanoparticle by Endophytic Ascomycetes Fungi From Calotropis gigantea Seeds (L.) R. Br Kavi Praba A, V. Ramesh Kumar, Raghul Rajah S This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8758115/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The biosynthesis of monodispersed nanoparticles, along with the determination of potentially responsible biomolecules, is the major bottleneck in the area of bio-nanotechnology research. The current investigation focused to synthesize the Silver Nanoparticles (AgNPs) using an endophytic fungus isolated from Calotropis gigantea (L. Br) seed, located in Chennai, India. The fungus was identified for its morphological and molecular characteristics. The fungus identified as Pichia kudriavzevii strain based on 16SrRNA sequencing. The produced particles were spherical shaped in nature and shows the surface plasma resonances 10nm. FTIR spectra of nanoparticle showed the C = C bonding, N-compound, C-O stretching vibration denotes the presences of alkene, protein and aromatic compound on the surface of silver nanoparticle produced by Pichia kudriavzevii . Synthesised NanoParticle revealed thee mean negative zeta potential and electrophoretic mobility of -29.8 mV and − 0.000255 cm 2 /Vs respectively. The nanoparticles produced were proteinaceous compounds as capping agents with − 29.8mV zeta potential and revealed the antibacterial activity against gram positive bacterial strain. Biosynthesis Endophytic fungus Calotropis gigantea seed copper nanoparticles SEM XRD Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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The current investigation focused to synthesize the Silver Nanoparticles (AgNPs) using an endophytic fungus isolated from \u003cem\u003eCalotropis gigantea (L. Br)\u003c/em\u003e seed, located in Chennai, India. The fungus was identified for its morphological and molecular characteristics. The fungus identified as \u003cem\u003ePichia kudriavzevii\u003c/em\u003e strain based on 16SrRNA sequencing. The produced particles were spherical shaped in nature and shows the surface plasma resonances 10nm. FTIR spectra of nanoparticle showed the C\u0026thinsp;=\u0026thinsp;C bonding, N-compound, C-O stretching vibration denotes the presences of alkene, protein and aromatic compound on the surface of silver nanoparticle produced by \u003cem\u003ePichia kudriavzevii\u003c/em\u003e. Synthesised NanoParticle revealed thee mean negative zeta potential and electrophoretic mobility of -29.8 mV and \u0026minus;\u0026thinsp;0.000255 cm\u003csup\u003e2\u003c/sup\u003e/Vs respectively. 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