Physiological responses of Holstein calves to prolonged heat stress and dietary supplementation with a postbiotic from Aspergillus oryzae

Physiological responses of Holstein calves to prolonged heat stress and dietary supplementation with a postbiotic from Aspergillus oryzae | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Physiological responses of Holstein calves to prolonged heat stress and dietary supplementation with a postbiotic from Aspergillus oryzae A. G. Ríus, J. D. Kaufman, I. R. Ipharraguerre, M. M. Li, M. D. Hanigan This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-957909/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 28 Jan, 2022 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract Increased ambient temperature causes heat stress in mammals, which affects physiological and molecular functions. We have recently reported that the dietary administration of a postbiotic from Aspergillus oryzae (AO) improves tolerance to heat stress in fruit flies and cattle. Furthermore, heat-induced gut dysfunction and systemic inflammation have been ameliorated in part by nutritional interventions. The objective of this study was to characterize the phenotypic response of growing calves to long-term heat stress compared to thermoneutral ad libitum fed and thermoneutral feed-restricted counterparts and examining the physiologic alterations associated with the administration of the AO postbiotic to heat-stressed calves with emphasis on intestinal permeability. In this report, we expand previous work by first demonstrating that heat stress reduced partial energetic efficiency of growth in control (45%) but not in AO-fed calves (62%) compared to thermoneutral animals (66%). While heat stress increased 20% the permeability of the intestine, AO postbiotic and thermoneutral treatments did not affect this variable. In addition, AO postbiotic reduced fecal water content relative to thermoneutral and heat stress treatments. Heat stress increased plasma concentrations of serum amyloid A, haptoglobin and lipocalin-2, and administration of AO postbiotic did not ameliorate this effect. In summary, our findings indicated that heat stress led to reduced nutrient-use efficiency and increased systemic inflammation. Results suggest that the AO postbiotic improved energy-use efficiency, water absorption, and the intestinal permeability in heat stress-mediated increase in gut permeability but did not reduce heat stress-mediated rise in markers of systemic inflammation. Animal Physiology Gastroenterology & Hepatology Biotechnology and Bioengineering fruit flies cattle heat-induced gut dysfunction systemic inflammation Full Text Additional Declarations No competing interests reported. Supplementary Files RiusetalSupplementaryinformation.pdf Cite Share Download PDF Status: Published Journal Publication published 28 Jan, 2022 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Major revision 30 Nov, 2021 Reviews received at journal 29 Nov, 2021 Reviews received at journal 25 Nov, 2021 Reviewers agreed at journal 16 Nov, 2021 Reviewers agreed at journal 14 Nov, 2021 Reviewers invited by journal 28 Oct, 2021 Editor assigned by journal 28 Oct, 2021 Editor invited by journal 14 Oct, 2021 Submission checks completed at journal 14 Oct, 2021 First submitted to journal 06 Oct, 2021 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-957909","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":55731259,"identity":"86c42fc2-720f-4fe2-806c-f59a9209c130","order_by":0,"name":"A. G. 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