Roles of Interleukin-1 Receptor Type II in the Pathogenesis of Endometriosis

Objective:To investigate the roles of Interleukin-1 receptor type II (IL-1RII) in the pathogenesis of endometriosis. Methods:Endometrial cells cultured in vitro were exposed to soluble IL-1RII or the recombinant adenovirus of IL-1RII (rAd-RII) for 12 hours,and then cell culture media were collected. The levels of IL-6 and IL-8 in the culture media were measured via enzyme-linked immunosorbent assay (ELISA),and the differential expression proteins were identified by two-dimensional electrophoresis and MALDI-TOF mass spectrometry after rAd-RII exposure. Results:Soluble IL-1RII (2.0 μg/ml) significantly inhibited the secretion of IL-6 and IL-8 in endometrial cells. The secretion of IL-8 induced by IL-1β in endometrial cells infected with rAd-RII was significantly lower than the PBS and rAd-LacZ control groups,while there were no significant differences of IL-6 secretion. 62 spots of differential expression proteins were collected through two-dimensional electrophoresis and 34 differential expression proteins were identified by MALDI-TOF mass spectrometry. The majority of these identified proteins were related to cellular metabolism and proliferation. Conclusion:These results suggest that IL-1RII can counteract the effects of IL-1β on endometrial stromal cells and may provide a new clinical strategy for the treatment of endometriosis.