Spectroscopic Analysis and Antibacterial Activity of Partially Purified Dichloromethane Fraction of Emilia praetermissa Milne-Redhead (Asteraceae) Leaf

Spectroscopic Analysis and Antibacterial Activity of Partially Purified Dichloromethane Fraction of Emilia praetermissa Milne-Redhead (Asteraceae) Leaf | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Spectroscopic Analysis and Antibacterial Activity of Partially Purified Dichloromethane Fraction of Emilia praetermissa Milne-Redhead (Asteraceae) Leaf Beauty Ighomena, Emmanuel Eimiomodebheki Odion This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6568149/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Purpose The search for better antibacterial agents from plants has resulted in the identification of molecules with existing analogues. Emilia praetermissa is an herbaceous plant that is traditionally used to treat bacterial skin infections and diarrhea. This study aimed to identify compounds in partially purified E. praetermissa and investigate their antibacterial activity. Methods Gradient elution chromatography was used to achieve partial purification of the compounds. Gas chromatography‒mass spectrometry (GCMS) and Fourier transform infrared spectroscopy were used to identify the phytochemicals from the eluent of interest (EP1). The agar well diffusion plate protocol enabled the assessment of the susceptibility profile of the eluents (EP1–EP11) (10 mg/mL) against clinical isolates: Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus . Results EP1 revealed fourteen compounds: 4aS-3,4,5,5,9-tetramethyl-1,25,6,7,8-hexahydro-4a,8-epoxybenzo[7[annulene (15.74%), N-(3-allyl-2-oxo-2,3-dihydro-1,3-b enzothiazol-6-yl) acetamide (5.52%), pentadecane (1.87%), heptacosane (3.52%), heptadecane (1.34%), nonadecane (1.09%), eicosane (3.56%), heneicosane (1.31%), di-sec-butyl phthalate (46.92%), bis(2-ethylhexyl) phthalate (6.62%), 2,4-di-tert-butylphenol (7.90%) and 2-ethyl-2H-benzotriazole (4.51%). The functional groups identified include O-H, N-H, C-H, C-C and C = C, which combine with the data from the GC‒MS. Susceptibility testing revealed the inhibition of Staphylococcus aureus , Bacillus subtilis and Escherichia coli by eluents EP1–EP11, excluding EP4, EP5 and EP10. Conclusion The results show that the identified compounds inhibit the pathogenic bacteria responsible for inducing skin infections and diarrhea, confirming their traditional use. Medicinal Chemistry Emilia praetermissa Staphylococcus aureus Escherichia coli Bacillus subtilis gradient elution Figures Figure 1 Figure 2 Figure 3 Introduction Emilia praetermissa Milne Redhead (Asteraceae) is a non-persistent vascular plant with origin in West Africa and is widely distributed on other continents, such as the Caribbean and Asia [ 1 – 3 ], where it was initially misidentified as Emilia forsbegii [ 1 ].It is habitually known for its invasive nature in both cultivated and fallowed lands. According to reports locally, E. praetermissa has been found to be very effective in the treatment of internal and external wounds, bacterial diarrhea, and respiratory infections and in preventing new-born skin infections [ 4 ]. Bacteria have been implicated in several skin infections and diarrheal-related diseases [ 5 ]. These entities are microscopic, ubiquitous, single-cell organisms that are broadly classified as gram-positive and gram-negative [ 6 ]. Monoderm bacteria are characterized by a thick peptidoglycan cell wall, whereas diderm bacteria possess an outer cell membrane [ 7 ]. Many of these bacteria can be found within and outside the body, where they have either beneficial or harmful effects. Some examples of monoderm include Staphylococcus aureus and Bacillus subtilis , whereas diderm contain Pseudomonas aeruginosa , Escherichia coli and Klebsiella pneumoniae [ 8 ]. S. aureus are cocci measuring 1 µm in diameter and are distinguished from other species in the genus by their coagulase-positive nature, salt leniency and regular hemolysis [ 9 ]. It has been implicated in several forms of infection, extending from shallow skin lesions to profound seated and nosocomial infections. It has also been reported to cause spoilage in food by releasing enterotoxin into foodstuff and responsible for toxic shock syndrome in human through super antigens release into the blood [ 10 – 12 ]. S. aureus colonizes the skin and nasal passage while exerting its virulence by releasing surface proteins that promote the colonization of host tissue [ 13 ]. The release of capsules and immunoglobulin-binding protein A promote phagocytosis and toxin release, which damage host tissues [ 14 – 15 ]. E. coli are facultative anaerobic rods that inhabit the gastrointestinal tract in human, where it forms part of the normal microbiota [ 16 – 17 ]. It exists singly or in pairs measuring 0.5–1.0 µm × 1.0–4.0 µm in width and length, do not form protective covering after exposure to harsh conditions and non-acid fast [ 18 ]. Although many E. coli strains are considered safe, though some serovar may cause running stomach when taken into the gastrointestinal tract; others may cause anaemia, while infections may be observed in some body organs and tracts [ 18 ]. Virulence may be acquired in E. coli by bacteriophage and plasmid formation, with pathogenicity islands encoding virulence factors and transporons within a genome [ 19 ]. The search for better antibacterial agents has necessitated the study of plant materials because of their believed safety, low untoward effects and cost effectiveness. Extracts from plants are known to contain phytochemicals with similar or better analogues than existing drugs. The antibacterial nature of the leaf extract of E. praetermissa was established in a previous study by our group, and GC‒MS analysis was also used to obtain a list of identified phytocompounds with likely antibacterial activities [ 20 ]. This study intends to narrow downthe identified compounds by partial purification of the dichloromethane fraction from the extract that has previously shown antibacterial activity. This study provides information on compounds with antibacterial potential in E. praetermissa ; thus, this research aims to identify compounds with antibacterial potential from the leaves of E. praetermissa. Materials and methods Solvents, Reagent, Drugs and Instruments Solvent and reagents used in this study were of analytical grade, while drug used was gotten from manufacturer of repute. Methanol (99%) (BHD), dichloromethane (BHD), n-hexane (BHD), Silica gel (Himedia GRM7477), KBr (99 -100.2%) (Merck), Mueller-Hunton Agar (Babio), Ciprofloxacin (Cipla), GC (7890A Agilent US model) - MS (MSD5675C), FTIR (Perkin Elmer 3000 MX). Collection, identification and preparation of the plants Fresh aerial parts of E. praetermissa were collected in October within the hours of 7:00 am − 8:30 am at the University of Benin, Ugbowo Campus, with geo-references at latitude 6 0 23’ 30”N and longitude 5 0 36’ 30”E. Identification and authentication were performed by Professor Emmanuel Aigbokhan of the Plant Biology and Biotechnology Department. The herbarium voucher number was UBH-E407, while further authentication was performed by uploading the picture to Google image service. A specimen of E. praetermissa was placed in the herbarium for prospective reference. The leaves were carefully separated from the stem and air dried away from direct sunlight for a duration of 25 days. The sample was reduced to a fine powder with the aid of an electric milling machine, and airtight container was used to store the pulverized sample until further work was carried out. Extraction and Partial Purification Extraction was performed via maceration using 2x2.5 L of methanol and 400 g of the powdered sample. This setup was left for 48 h with stirring at intervals of 12 h; this mixture was subsequently decanted into a 3 L beaker and passed through size 1 filter paper. An in-vacuum rotary evaporator maintained at a temperature of 50°C was used to concentrate the filtrate, and the weight of the extract obtained was noted (35.00 g) and subsequently kept in a refrigerator at 4°C until use. The crude extract (26.00 g) was adsorbed to 25 g of silica gel (mesh size 60–120 µm) until it became powdery, after which it was transferred into a column (measuring 5 cm × 50 cm) packed with silica gel (200 g). N-hexane (600 mL) was isocratically used to elute the column, and the eluent was collected. This process was repeated by gradient elution using n-hexane-dichloromethane at different ratios: 90:10, 80:20, 70:30, 60:40, 50:50, 40:60, 30:70, 20:80, 10:90 and 0:100. Each variation in solvent mixture resulted in a total volume of 300 mL and a total of 292 test tubes used to collect the eluent from the column. These samples were observed to have different colours ranging from yellow to deep green and pale green. Crystals were observed in test tubes labelled 41–67 (EP1); hence, they became our focus. The white crystals were subjected to gas chromatography‒mass spectrometry (GC‒MS) and Fourier transform infrared (FTIR) spectroscopic analysis, and the antibacterial potential of the eluents (EP1–EP11) was evaluated. GC‒MS analysis : This analysis was carried out via coupled GC‒MS with a triple-axis detector and a 10 µL syringe autosampler. The GC system (7890A) was an Agilent USA model, while the MS was an inert MSD 5675C. A capillary column from Agilent (19091-433HP-5Ms) treated with 5% phenyl, 95% methyl polysiloxane and measuring 30 m (length), 0.2 µm (internal diameter), and 250 µm (thickness) was utilized for chromatographic separation, while helium was the carrier gas. The ion source and interface temperatures were set at 250°C and 300°C, 16.2 psi as the pressure and a 1:50 split ratio with a 1 µl injector in split mode, while 280°C was the injection temperature. The temperature of the column was started at 50°C for 2 min and adjusted at a rate of 20°C/min to 100°C. This mixture was elevated to 250°C at 20°C/min and maintained for 5 min. While acquiring data, software from the manufacturer was utilized to control the system, and compound qualification was achieved by mass spectra comparison with data from previous research and NIST [ 21 ]. FTIR Spectroscopy Functional group determination in EP1 was performed via a Perkin Elmer 3000 MX spectrometer. This process involves mixing a few crystals of EP1 with potassium bromide and reducing them to a fine homogenous powder in a mortar (agate). This formed an appropriate pellet following the application of a pressure of 6350 kg. The spectra were recorded following scanning in the wavenumber range between 4000 cm − 1 and 650 cm − 1 . The resolution was set at 4 cm − 1, and the scan per spectrum was 32 cm − 1 . Analysis of the infrared spectra was performed via WIN-IR Pro Version 3.0, with a sensitivity of the peak at 2 cm − 1 . Antimicrobial activity Preparation of media and agar plates A total of 38 g of Mueller–Hinton agar was added to 1 L of distilled water in a conical flask, and this mixture was heated to 100°C while stirring. The mixture was covered with foil paper and autoclaved for 15 min at 121°C. The mixture was allowed to cool to 45°C and subsequently dispensed (30 mL) under a sterile lamp into Petri dishes, while the plates were allowed to set and solidify. The samples were dried in a hot air oven at 50°C for 10 minutes to remove excess surface moisture. Collection and preparation of bacterial cultures The drug-resistant clinical isolate of the bacteria used for this study was obtained from the University of Benin Teaching Hospital and was cultured overnight (24 h at 37°C) in sterile nutrient broth by inoculation. The bacteria used for this study included S. aureus, K. pneumoniae, E. coli, B. subtilis and P. aeruginosa . Identical colonies from these plates were inoculated into sterile nutrient broths. These mixtures were allowed to incubate for 12 h and subsequently adjusted with the MacFarland standard (0.5%) to obtain an inoculum size of 108 cfu/mL. These concentrations were further adjusted to 106 cfu/mL by diluting the inoculum to 1:100. The modified agar well diffusion method was used in the susceptibility test; this was achieved by thoroughly mixing 200 µL of standard inoculum with 30 mL of sterile Mueller-Hinton agar, which was allowed to solidify. A sterile cork borer (8 mm or 10 mm in diameter) was used to cut the wells into the agar plate. The base of each well was sealed with molten Mueller-Hinton agar, and a calibrated micropipette with a rubber teat was used to introduce 10 mg/mL of the eluent (EP1–EP11) into each well. The plates were allowed to stand for 30 min before being incubated upside down at 37°C for 24 h. Inhibition zone diameters (measured in millimetres) of each eluent were measured and recorded. A similar protocol was repeated for the control, ciprofloxacin (0.5 mg/mL) [ 22 ]. Statistical analysis The antimicrobial test was performed in duplicate, and the data are presented as the means ± standard errors of the means. The data were analysed via one-way analysis of variance. A post hoc study was performed via Tukey analysis, and the level of significance was set at p ≤ 0.05 (***), using version 5 of the GraphPad Prism. Results and Discussion Following extraction, the crude extract was adsorbed onto silica gel, and different solvent ratios (dichloromethane–hexane) were used to elute the prepared column. The eluents were collected in test tubes (Table 1 ), which were subsequently bulked (EP1–EP11) together on the basis of thin-layer chromatographic profiles (Table 2 ). Figure 1 show the scheme from the extraction of the powdered plant to the point where the eluents were obtained. Table 1 partially purified crude extract of Emilia praetermissa Solvent mix Solvent ratio Volume (mL) Test tubes Hexane 100 600 1–40 Hexane-dichloromethane 90:10 300 41–67 Hexane-dichloromethane 80:20 300 68–91 Hexane-dichloromethane 70:30 300 92–118 Hexane-dichloromethane 60:40 300 119–142 Hexane-dichloromethane 50:50 300 143–169 Hexane-dichloromethane 40:60 300 170–193 Hexane-dichloromethane 30:70 300 194–219 Hexane-dichloromethane 20:80 300 220–242 Hexane-dichloromethane 10:90 300 243–269 Dichloromethane 100 300 270–292 Table 2 Coding of eluents from test tubes Coding Test tube EP1 41–67 EP2 68–80 EP3 81–96 EP4 97–111 EP5 112–143 EP6 144–157 EP7 158–197 EP8 198–224 EP9 225–240 EP10 241–268 EP11 269–292 Susceptibility test The antibacterial potential of the eluents was evaluated against five bacterial species, B. subtilis, E. coli, K. pneumoniae, S. aureus and P. aeruginosa , via the agar well diffusion method previously described by Babaiwa and coworkers [ 22 ]. Ciprofloxacin was utilized as the reference drug at 0.5 mg/mL. E. coli , B. subtilis and S. aureus were susceptible to 10 mg/ml EP1-EP4, EP6-EP9 and EP11, respectively (Table 3 ). The zone of inhibition was 12 ± 0.12 mm (EP4) for B. subtilis , ranging from 12 mm to 14 mm (EP1-EP3, EP6-EP9 and EP11) for E. coli and 14 mm to 18 mm (EP1-EP3, EP6-EP9 and EP11) for S. aureus . The effects of the EP2, EP3, EP7 and EP10 strains tested against E. coli were significant compared with those of ciprofloxacin, with a level of significance at p ≤ 0.05. Similarly, EP2, EP6 and EP11 were significantly different at p ≤ 0.05 compared with the standard drug with S. aureus . Resistance of B. subtilis , EP4, EP5 and EP10 to both E. coli and S. aureus was observed with the EP1-EP3 and EP5-EP11 eluents . While maintaining similar concentrations, resistance was observed against P. aeruginosa and K. pneumoniae via EP1 - EP11. Eluents that are more susceptible than ciprofloxacin (0.5 mg/mL) are known, although our previous study revealed the susceptibility of the E. praetermissa dichloromethane fraction to P. aeruginosa [ 20 ]. This study further purified the dichloromethane fraction into various eluents (EP1 to EP11), and the activities against S. aureus , B. subtilis and E. coli were noted. Resistance may have been observed in gram-negative bacteria, but susceptibility to E. coli indicated that these eluents may possess specific related antibacterial actions. The specific mechanism by which these genes confer resistance could involve efflux pump action, permeability barrier induction, mutational or recombination changes in target enzymes and alterations in targets with decreased affinity for drug compounds [ 23 ]. Additionally, resistance could hypothetically be due to virulence [ 24 – 25 ]. The susceptibility of S. aureus, E. coli and B. subtilis to these eluents could imply their use in the treatment of skin infection and diarrhea caused by these bacteria, justifying their ethno-medicinal usage of this plant. Table 3 Antibacterial activity Bacteria EP1 EP2 EP3 EP4 EP5 EP6 EP7 EP8 EP9 EP10 EP11 Ciprofloxacin Escherichia coli 14 ± 0.20 13 ± 0.11 12 ± 0.11 NZ NZ 13 ± 0.10 14 ± 0.10 13 ± 0.11 13 ± 0.12 NZ 13 ± 0.00 13 ± 0.11 Klebsiella pneumoniae NZ NZ NZ NZ NZ NZ NZ NZ NZ NZ NZ 22 ± 0.20 Pseudomonas aeruginosa NZ NZ NZ NZ NZ NZ NZ NZ NZ NZ NZ 30 ± 0.14 Staphylococcus aureus 16 ± 0.10 18 ± 0.11 15 ± 0.21 NZ NZ 18 ± 0.10 18 ± 0.10 18 ± 0.08 18 ± 0.05 NZ 14 ± 0.20 26 ± 0.12 Bacillus subtilis NZ NZ NZ 12 ± 0.12 NZ NZ NZ NZ NZ NZ NZ 30 ± 0.22 Keywords: NZ = No zone of inhibition; zones of inhibition were measured in millimetres. FTIR analysis The functional groups in the EP1 eluent were determined from the absorption spectrum from the FTIR data. The absorption spectrum is provided in Fig. 2 , while a summary of the absorption range and specific functional groups in EP1 are provided in Table 4 . The main functional groups include the O‒H bond of the hydroxyl group and the N‒H medium bond of the secondary amine and amide. C-H and C-C stretches were observed for saturated alkanes and rings. The C = C bonds and C ≡ C stretches are indicative of alkene and alkyne groups. The H-C = O stretch for the aldehydric group and overtones was observed at 1919.60 cm − 1 , although it was weak. Towards the end of the group frequency region, N-O symmetric stretching and C-O stretching were observed, representing nitrile and aldehydric, amide and ester groups, respectively. Table 4 FTIR spectra interpretation S/N Wavenumber (Reference) Wavenumber (Test Sample) Functional group assignment Inference 1 3650 − 3200 3336.00 O-H, N-H N-H medium, H-bonded 2 3000 − 2850 2974.40 C-H C-H stretching bond 3 3000 − 2850 2929.70 C-H C-H stretching bond 4 2830 − 2695 2832.80 H-C = O H-C = O stretch 5 2260 − 2100 2124.60 -C ≡ C- C ≡ C stretch 6 2000 − 1665 1919.60 Overtones, weak from this range 7 1680 − 1620 1636.30 C = C C = C bonds 8 1500 − 1400 1449.90 C-C C-C stretch in ring 9 1360 − 1290 1326.90 N-O N-O symmetric stretch 10 1260 − 1050 1088.40 C-O C-O stretch 11 1300 − 1000 1028.40 C-O C-O stretch 12 1000 − 650 879.70 =C-H =C-H bend GC‒MS analysis The chromatogram of EP1 revealed fourteen compounds (peaks) (Fig. 2 ), with retention times ranging from 4.792 min to 19.944 min. The prominent compounds were observed at 9.319 min, 9.719 min, 13.662 min, 13.902 min, and 19.944 min, with a total percentage area of 82.70%, while the individual percentage areas were as follows: 15.74%, 7.90%, 5.52%, 46.92%, and 6.62%, respectively. These fourteen compounds are mainly triazole, straight-chain alkanes, epoxide, amide and phthalate (Table 3 ). The prominent compounds are 2-ethyl-2H-benzotriazole (4.51%), N-(3-Allyl-2-oxo-2,3-dihydro-1,3-b enzothiazol-6-yl) acetamide (5.52%), bis(2-ethylhexyl) phthalate (6.62%), 2,4-di-tert-butylphenol (7.90%), (4aS)-3,5,5,9-tetramethyl-1,2,5,6,7,8-hexahydro-4a,8-epoxybenzo[ 7 ]annulene (15.74%) and di-sec-butyl phthalate (46.92%). These findings indicate a high level of phthalates in EP1 (53.54%). In a previous study, bis-(2-ethylhexyl) phthalate was shown to have antibacterial activity against E. coli and S. aureus [ 27 ]. In contrast, di-sec-butyl phthalate, which is isolated from the whole plant of Begonia malabarica (Begoniaceae), has shown antibacterial potential against K. pneumoniae, P. aeruginosa and E. coli at 100 mg/mL [ 28 ]. 2,4-Di-tert-butylphenol purified from the Streptomyces species KCA1 from Phyllanthus niruri inhibited S. aureus and E. coli at 0.78 µg/mL and 50 µg/mL [ 29 ]. These findings contradict those of this study, especially with respect to the inhibition of K. pneumonia and P. aeruginosa . Table 5 Compounds identified from EP1 from the retention time, percentage area and molecular weight S/N Retention Time (min) Percentage Area Base peak Molecular weight Compound 1 4.792 4.51 91.0 147.0 2-ethyl-2H-Benzotriazole 2 7.013 1.09 57.0 268.5 Nonadecane 3 9.319 15.74 161.0 218.3 (4aS)-3,5,5,9-Tetramethyl-1,2,5,6,7,8-hexahydro-4a,8-epoxybenzo[ 7 ]annulene 4 9.427 1.87 57.0 212.4 Pentadecane 5 9.719 7.90 191.0 206.3 2,4-Di-tert-butylphenol 6 11.539 1.77 57.0 280.7 Heptacosane 7 11.951 1.34 57.0 240.5 Heptadecane 8 13.427 1.85 57.0 280.7 Heptacosane 9 13.662 5.52 231.0 248.3 N-(3-Allyl-2-oxo-2,3-dihydro-1,3-b enzothiazol-6-yl) acetamide 10 13.902 46.92 149.0 278.3 Di-sec-butyl phthalate 11 15.138 2.33 57.0 282.5 Eicosane 12 15.464 1.23 57.0 282.5 Eicosane 13 17.026 1.31 57.0 296.6 Heneicosane 14 19.944 6.62 149.0 390.6 Bis(2-ethylhexyl) phthalate Savelli and coworkers [ 30 ] reported the use of derivatives of 2-ethyl-2H-benzotriazole as antimicrobial agents, whereas Nuvole et al . [ 31 ] reported the susceptibility of E. coli to acidic derivatives of benzotriazole. This compound is made of a benzene ring fused to a triazole; this triazole ring possesses antimicrobial activity, which can be partially or completely lost following annulation of the triazole ring at any point [ 32 ]. Pentadecane, heptadecane, eicosane, heneicosane and nonadecane, with a percentage area of 12.79%, are straight alkanes; some have previously been reported in E. coccinea [ 33 ] to have antimicrobial activity [ 34 ]. Derivatives of nonadecane have been reported to have moderate antibacterial effects against E. coli, S. aureus, P. aeruginosa and B. subtilis [ 35 – 36 ]. The presence of these compounds in the eluent EP1 may be responsible for the antibacterial activity observed and could be synergistic in action against these bacteria. Conclusion Gradient eluents from hexane-dichloromethane of E. praetermissa were shown to possess antibacterial activity against selected clinical isolates. Antibacterial activity was observed in the EP1 eluent because of the presence of long-chain alkanes, benzotriazole, phthalate and butylphenol derivatives. These findings validate the use of E. praetermissa in the treatment of skin infection and diarrhea, since some of the bacteria involved in these conditions are inhibited. Declarations Contribution of Authors Beauty Ighomena was involved in data collection and editing of the manuscript, whereas Emmanuel Eimiomodebheki Odion conceptualized the study and wrote and edited the manuscript. Acknowledgement: The authors wish to especially thank Mr. Wilfred Aisagbonbuomwan, a laboratory staff member of the Department of Pharmaceutical Microbiology, for assisting with the microbial tests. Conflict of interest: There was no conflict of interest before, during or after the study. Funding: The success of this work was due to their personal contributions. Funding was not received throughout the study duration. 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Palgrave Macmillan, London. (1985). https://doi.org/10.1007/978-1-349-07834-9_12. Craig, R., Rayner, C.R. & Munckhof, W.J. Antibiotics Currently used in The Treatment of Infections Caused by Staphylococcus aureus . Internal Medicine Journal 35.2(2):S3-16. (2006). https://doi.org/10.1111/j.1444-0903.2005.00976.x. Javed, M.R., Salman, M., Tariq, A., Tawab, A., Zahoor, M.K., Naheed, S., Shahid, M., Ijaz, A. & Ali, H. Antibacterial and Larvicidal Potential of Bis-(2-Ethylhexyl) Phthalate from Lactiplantibacillus plantarum . Molecules 25; 27(21): 7220 (2022). doi: 10.3390/molecules27217220. Shobi, T.M. & Viswanathan, M.B.G. Antibacterial Activity of di-butyl phthalate Isolated from Begonia malabarica . Journal of Applied Biotechnology and Bioengineering 5(2): 97–100 (2018). doi: 10.15406/jabb.2018.05.00123. Seenivasan, A., Manikkam, R., Kaari, M., Sahu, A.K., Said, M. & Dastager S.G. 2,4-Di-tert-butylphenol (2,4-DTBP) Purified from Streptomyces sp. KCA1 from Phyllanthus niruri : Isolation, characterization, and antibacterial and anticancer properties. Journal of King Saud University - Science 34, 5, 102088 (2022). https://doi.org/10.1016/j.jksus.2022.102088. Savelli, F., Pau, A., Boido, A. & Sparatore F. Aminoalkyl Derivatives of Benzotriazole and Naphthotriazole. Bollettino Chimico Farmaceutico 127:144–147 (1988). Nuvole, A., Sanna, P., Paglietti, G., Juliano, C., Zanetti, S. & Cappuccinelli P. 1,2,3-triazolo[4,5-f]quinolines. II. Preparation and antimicrobial evaluation of 6-ethyl-6,9-dihydro-1(2)(3)-R-1(2) (3)H-triazolo [4,5-f]quinolin-9-one-8-carboxylic acids as anti-infectives of the urinary tract (1). Farm Science 44:619–632 (1989). Sanna, P., Carta, A., Paglietti, G., Zanetti, S. & Fadda G. 1,2,3-Triazolo[4,5-h]quinolines. III. Preparation and Antimicrobial Evaluation of 4-ethyl-4,7-dihydro-1(2)H Triazolo [4,5-h]quinolin-7-one-6-carboxylic acids as Anti-infectives of The Urinary Tract. Farm Science 47:1001–1019 (1992). Mihigo, S.O., Tekayela, M., Masesane, I.B. & Sichilongo, K. Preliminary GC‒MS Profiling and Antibacterial Activity Investigation of Emilia coccinea (Sims) G. Don (Asteraceae). International Journal of Chemistry and Aquatic Sciences 1(2) 31-36 (2015). Begum, I.F., Mohankumar, R., Jeevan, M. & Ramani, K. GC‒MS Analysis of Bio-Active Molecules Derived From Paracoccus pantotrophus FMR19 and The Antimicrobial Activity Against Bacterial Pathogens and MDROs. Indian Journal of Microbiology 56:426–32 (2016). https://doi.org/10.1007/s12088-016-0609-1. Marrero, J., Rodrı´guez, I.I. & Rodrı´guez, A.D. The Natural Products Chemistry of the Gorgonian Genus Pseudopterogorgia (Octocorallia: Gorgoniidae) University of Puerto Rico, San Juan, PR, USA. Elsevier. 407 (2010). Naeim, H., El-Hawiet, A., Abdel Rahman, R.A, Hussein, A., El Demellawy, M.A. & Amira M. . Antibacterial Activity of Centaurea pumilio L. Root and Aerial Part Extracts Against Some Multidrug Resistant Bacteria. BMC Complement Medicine and Therapies 20, 79 (2020). https://doi.org/10.1186/s12906-020-2876-y. Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6568149","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":450428559,"identity":"be6851c0-dbb6-4e32-ad19-7cfda57905c8","order_by":0,"name":"Beauty Ighomena","email":"","orcid":"","institution":"University of Benin","correspondingAuthor":false,"prefix":"","firstName":"Beauty","middleName":"","lastName":"Ighomena","suffix":""},{"id":450428560,"identity":"9b4f9ceb-dcef-4c49-8992-c9d75988d5fc","order_by":1,"name":"Emmanuel Eimiomodebheki Odion","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA/0lEQVRIiWNgGAWjYBADGSA2OMDAYMPAwMwDFmFsIKCFB6oljUQtQHwYysajhV/sdOLnwjYbHgb25o0HPu44n7idnffg4wIGG9kNB7BrkZydu1l6ZlsaDwPPsYKDM8/cTtzZzJdsPIMhzRiXFoPbuRukedsO8zBI5Bgc5m27nbjhMI+ZNA/D4URcWuxv527+zdv2H6blHEiL+W8ehv84tRhI524D2nIApuUA2BZgoB3AqUXidu42a55zyTxsYL+0JRtvOMyXLM1jkGw8E4cWfqD3b/OU2cnxszdv/vCxzU52w/mzBz/zVNjJ9uHQAgdsaA4moHwUjIJRMApGAV4AAH9VW81yanOqAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0002-0893-5367","institution":"University of Benin","correspondingAuthor":true,"prefix":"","firstName":"Emmanuel","middleName":"Eimiomodebheki","lastName":"Odion","suffix":""}],"badges":[],"createdAt":"2025-04-30 23:01:48","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-6568149/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6568149/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":81807935,"identity":"a036734a-e0ff-40ea-8780-d7a2018bbf98","added_by":"auto","created_at":"2025-05-02 07:57:19","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":451865,"visible":true,"origin":"","legend":"\u003cp\u003eSee image above for figure legend.\u003c/p\u003e","description":"","filename":"1.png","url":"https://assets-eu.researchsquare.com/files/rs-6568149/v1/cc540ec8c0d2349e4b2f0b6b.png"},{"id":81807933,"identity":"472ce618-3cac-4e7b-b406-d26c57ca0f8a","added_by":"auto","created_at":"2025-05-02 07:57:19","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":44826,"visible":true,"origin":"","legend":"\u003cp\u003eFTIR spectraof EP1\u003c/p\u003e","description":"","filename":"floatimage2.png","url":"https://assets-eu.researchsquare.com/files/rs-6568149/v1/e67d2eece748f0d76e8cacd4.png"},{"id":81807932,"identity":"d6ed4e3d-0a1c-44aa-a0fa-4d0d5329d451","added_by":"auto","created_at":"2025-05-02 07:57:19","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":64520,"visible":true,"origin":"","legend":"\u003cp\u003eChromatogram of EP1\u003c/p\u003e","description":"","filename":"floatimage3.png","url":"https://assets-eu.researchsquare.com/files/rs-6568149/v1/f0a6c1e83804f352b8818bba.png"},{"id":81809093,"identity":"efe585a9-ee99-4083-b376-b965dbe703da","added_by":"auto","created_at":"2025-05-02 08:21:19","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1520535,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6568149/v1/b6f638ae-7bce-488b-b2b6-1d56955835f6.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003e\u003cstrong\u003eSpectroscopic Analysis and Antibacterial Activity of Partially Purified Dichloromethane Fraction of \u003c/strong\u003e\u003cem\u003e\u003cstrong\u003eEmilia praetermissa\u003c/strong\u003e\u003c/em\u003e\u003cstrong\u003e Milne-Redhead (Asteraceae) Leaf\u003c/strong\u003e\u003c/p\u003e","fulltext":[{"header":"Introduction","content":"\u003cp\u003e \u003cem\u003eEmilia praetermissa\u003c/em\u003e Milne Redhead (Asteraceae) \u003cem\u003eis\u003c/em\u003e a non-persistent vascular plant with origin in West Africa and is widely distributed on other continents, such as the Caribbean and Asia [\u003cspan additionalcitationids=\"CR2\" citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e], where it was initially misidentified as \u003cem\u003eEmilia forsbegii\u003c/em\u003e [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e].It is habitually known for its invasive nature in both cultivated and fallowed lands. According to reports locally, \u003cem\u003eE. praetermissa\u003c/em\u003e has been found to be very effective in the treatment of internal and external wounds, bacterial diarrhea, and respiratory infections and in preventing new-born skin infections [\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eBacteria have been implicated in several skin infections and diarrheal-related diseases [\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e]. These entities are microscopic, ubiquitous, single-cell organisms that are broadly classified as gram-positive and gram-negative [\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e]. Monoderm bacteria are characterized by a thick peptidoglycan cell wall, whereas diderm bacteria possess an outer cell membrane [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e]. Many of these bacteria can be found within and outside the body, where they have either beneficial or harmful effects. Some examples of monoderm include \u003cem\u003eStaphylococcus aureus\u003c/em\u003e and \u003cem\u003eBacillus subtilis\u003c/em\u003e, whereas diderm contain \u003cem\u003ePseudomonas aeruginosa\u003c/em\u003e, \u003cem\u003eEscherichia coli\u003c/em\u003e and \u003cem\u003eKlebsiella pneumoniae\u003c/em\u003e [\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e].\u003c/p\u003e \u003cp\u003e \u003cem\u003eS. aureus\u003c/em\u003e are cocci measuring 1 \u0026micro;m in diameter and are distinguished from other species in the genus by their coagulase-positive nature, salt leniency and regular hemolysis [\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e]. It has been implicated in several forms of infection, extending from shallow skin lesions to profound seated and nosocomial infections. It has also been reported to cause spoilage in food by releasing enterotoxin into foodstuff and responsible for toxic shock syndrome in human through super antigens release into the blood [\u003cspan additionalcitationids=\"CR11\" citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e]. \u003cem\u003eS. aureus\u003c/em\u003e colonizes the skin and nasal passage while exerting its virulence by releasing surface proteins that promote the colonization of host tissue [\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e]. The release of capsules and immunoglobulin-binding protein A promote phagocytosis and toxin release, which damage host tissues [\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e].\u003c/p\u003e \u003cp\u003e \u003cem\u003eE. coli\u003c/em\u003e are facultative anaerobic rods that inhabit the gastrointestinal tract in human, where it forms part of the normal microbiota [\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e]. It exists singly or in pairs measuring 0.5\u0026ndash;1.0 \u0026micro;m \u0026times; 1.0\u0026ndash;4.0 \u0026micro;m in width and length, do not form protective covering after exposure to harsh conditions and non-acid fast [\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e]. Although many \u003cem\u003eE. coli\u003c/em\u003e strains are considered safe, though some serovar may cause running stomach when taken into the gastrointestinal tract; others may cause anaemia, while infections may be observed in some body organs and tracts [\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e]. Virulence may be acquired in \u003cem\u003eE. coli\u003c/em\u003e by bacteriophage and plasmid formation, with pathogenicity islands encoding virulence factors and transporons within a genome [\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eThe search for better antibacterial agents has necessitated the study of plant materials because of their believed safety, low untoward effects and cost effectiveness. Extracts from plants are known to contain phytochemicals with similar or better analogues than existing drugs. The antibacterial nature of the leaf extract of \u003cem\u003eE. praetermissa\u003c/em\u003e was established in a previous study by our group, and GC‒MS analysis was also used to obtain a list of identified phytocompounds with likely antibacterial activities [\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e]. This study intends to narrow downthe identified compounds by partial purification of the dichloromethane fraction from the extract that has previously shown antibacterial activity. This study provides information on compounds with antibacterial potential in \u003cem\u003eE. praetermissa\u003c/em\u003e; thus, this research aims to identify compounds with antibacterial potential from the leaves of \u003cem\u003eE. praetermissa.\u003c/em\u003e\u003c/p\u003e"},{"header":"Materials and methods","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e \u003ch2\u003eSolvents, Reagent, Drugs and Instruments\u003c/h2\u003e \u003cp\u003eSolvent and reagents used in this study were of analytical grade, while drug used was gotten from manufacturer of repute. Methanol (99%) (BHD), dichloromethane (BHD), n-hexane (BHD), Silica gel (Himedia GRM7477), KBr (99 -100.2%) (Merck), Mueller-Hunton Agar (Babio), Ciprofloxacin (Cipla), GC (7890A Agilent US model) - MS (MSD5675C), FTIR (Perkin Elmer 3000 MX).\u003c/p\u003e \u003c/div\u003e\n\u003ch3\u003eCollection, identification and preparation of the plants\u003c/h3\u003e\n\u003cp\u003eFresh aerial parts of \u003cem\u003eE. praetermissa\u003c/em\u003e were collected in October within the hours of 7:00 am \u0026minus;\u0026thinsp;8:30 am at the University of Benin, Ugbowo Campus, with geo-references at latitude 6\u003csup\u003e0\u003c/sup\u003e 23\u0026rsquo; 30\u0026rdquo;N and longitude 5\u003csup\u003e0\u003c/sup\u003e 36\u0026rsquo; 30\u0026rdquo;E. Identification and authentication were performed by Professor Emmanuel Aigbokhan of the Plant Biology and Biotechnology Department. The herbarium voucher number was UBH-E407, while further authentication was performed by uploading the picture to Google image service. A specimen of \u003cem\u003eE. praetermissa\u003c/em\u003e was placed in the herbarium for prospective reference.\u003c/p\u003e \u003cp\u003eThe leaves were carefully separated from the stem and air dried away from direct sunlight for a duration of 25 days. The sample was reduced to a fine powder with the aid of an electric milling machine, and airtight container was used to store the pulverized sample until further work was carried out.\u003c/p\u003e\n\u003ch3\u003eExtraction and Partial Purification\u003c/h3\u003e\n\u003cp\u003eExtraction was performed via maceration using 2x2.5 L of methanol and 400 g of the powdered sample. This setup was left for 48 h with stirring at intervals of 12 h; this mixture was subsequently decanted into a 3 L beaker and passed through size 1 filter paper. \u003cem\u003eAn in-vacuum\u003c/em\u003e rotary evaporator maintained at a temperature of 50\u0026deg;C was used to concentrate the filtrate, and the weight of the extract obtained was noted (35.00 g) and subsequently kept in a refrigerator at 4\u0026deg;C until use.\u003c/p\u003e \u003cp\u003eThe crude extract (26.00 g) was adsorbed to 25 g of silica gel (mesh size 60\u0026ndash;120 \u0026micro;m) until it became powdery, after which it was transferred into a column (measuring 5 cm \u0026times; 50 cm) packed with silica gel (200 g). N-hexane (600 mL) was isocratically used to elute the column, and the eluent was collected. This process was repeated by gradient elution using n-hexane-dichloromethane at different ratios: 90:10, 80:20, 70:30, 60:40, 50:50, 40:60, 30:70, 20:80, 10:90 and 0:100. Each variation in solvent mixture resulted in a total volume of 300 mL and a total of 292 test tubes used to collect the eluent from the column. These samples were observed to have different colours ranging from yellow to deep green and pale green. Crystals were observed in test tubes labelled 41\u0026ndash;67 (EP1); hence, they became our focus. The white crystals were subjected to gas chromatography‒mass spectrometry (GC‒MS) and Fourier transform infrared (FTIR) spectroscopic analysis, and the antibacterial potential of the eluents (EP1\u0026ndash;EP11) was evaluated.\u003c/p\u003e \u003cp\u003e \u003cb\u003eGC‒MS analysis\u003c/b\u003e: This analysis was carried out via coupled GC‒MS with a triple-axis detector and a 10 \u0026micro;L syringe autosampler. The GC system (7890A) was an Agilent USA model, while the MS was an inert MSD 5675C. A capillary column from Agilent (19091-433HP-5Ms) treated with 5% phenyl, 95% methyl polysiloxane and measuring 30 m (length), 0.2 \u0026micro;m (internal diameter), and 250 \u0026micro;m (thickness) was utilized for chromatographic separation, while helium was the carrier gas. The ion source and interface temperatures were set at 250\u0026deg;C and 300\u0026deg;C, 16.2 psi as the pressure and a 1:50 split ratio with a 1 \u0026micro;l injector in split mode, while 280\u0026deg;C was the injection temperature. The temperature of the column was started at 50\u0026deg;C for 2 min and adjusted at a rate of 20\u0026deg;C/min to 100\u0026deg;C. This mixture was elevated to 250\u0026deg;C at 20\u0026deg;C/min and maintained for 5 min. While acquiring data, software from the manufacturer was utilized to control the system, and compound qualification was achieved by mass spectra comparison with data from previous research and NIST [\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e].\u003c/p\u003e \u003cp\u003e \u003cstrong\u003eFTIR Spectroscopy\u003c/strong\u003e \u003cp\u003eFunctional group determination in EP1 was performed via a Perkin Elmer 3000 MX spectrometer. This process involves mixing a few crystals of EP1 with potassium bromide and reducing them to a fine homogenous powder in a mortar (agate). This formed an appropriate pellet following the application of a pressure of 6350 kg. The spectra were recorded following scanning in the wavenumber range between 4000 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e and 650 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e. The resolution was set at 4 cm\u003csup\u003e\u0026minus;\u0026thinsp;1,\u003c/sup\u003e and the scan per spectrum was 32 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e. Analysis of the infrared spectra was performed via WIN-IR Pro Version 3.0, with a sensitivity of the peak at 2 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e.\u003c/p\u003e \u003c/p\u003e\n\u003ch3\u003eAntimicrobial activity\u003c/h3\u003e\n\u003cdiv id=\"Sec7\" class=\"Section2\"\u003e \u003ch2\u003ePreparation of media and agar plates\u003c/h2\u003e \u003cp\u003eA total of 38 g of Mueller\u0026ndash;Hinton agar was added to 1 L of distilled water in a conical flask, and this mixture was heated to 100\u0026deg;C while stirring. The mixture was covered with foil paper and autoclaved for 15 min at 121\u0026deg;C. The mixture was allowed to cool to 45\u0026deg;C and subsequently dispensed (30 mL) under a sterile lamp into Petri dishes, while the plates were allowed to set and solidify. The samples were dried in a hot air oven at 50\u0026deg;C for 10 minutes to remove excess surface moisture.\u003c/p\u003e \u003cp\u003eCollection and preparation of bacterial cultures\u003c/p\u003e \u003cp\u003eThe drug-resistant clinical isolate of the bacteria used for this study was obtained from the University of Benin Teaching Hospital and was cultured overnight (24 h at 37\u0026deg;C) in sterile nutrient broth by inoculation. The bacteria used for this study included \u003cem\u003eS. aureus, K. pneumoniae, E. coli, B. subtilis\u003c/em\u003e and \u003cem\u003eP. aeruginosa\u003c/em\u003e. Identical colonies from these plates were inoculated into sterile nutrient broths. These mixtures were allowed to incubate for 12 h and subsequently adjusted with the MacFarland standard (0.5%) to obtain an inoculum size of 108 cfu/mL. These concentrations were further adjusted to 106 cfu/mL by diluting the inoculum to 1:100.\u003c/p\u003e \u003cp\u003eThe modified agar well diffusion method was used in the susceptibility test; this was achieved by thoroughly mixing 200 \u0026micro;L of standard inoculum with 30 mL of sterile Mueller-Hinton agar, which was allowed to solidify. A sterile cork borer (8 mm or 10 mm in diameter) was used to cut the wells into the agar plate. The base of each well was sealed with molten Mueller-Hinton agar, and a calibrated micropipette with a rubber teat was used to introduce 10 mg/mL of the eluent (EP1\u0026ndash;EP11) into each well. The plates were allowed to stand for 30 min before being incubated upside down at 37\u0026deg;C for 24 h. Inhibition zone diameters (measured in millimetres) of each eluent were measured and recorded. A similar protocol was repeated for the control, ciprofloxacin (0.5 mg/mL) [\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e].\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec8\" class=\"Section2\"\u003e \u003ch2\u003eStatistical analysis\u003c/h2\u003e \u003cp\u003eThe antimicrobial test was performed in duplicate, and the data are presented as the means\u0026thinsp;\u0026plusmn;\u0026thinsp;standard errors of the means. The data were analysed via one-way analysis of variance. A post hoc study was performed via Tukey analysis, and the level of significance was set at p\u0026thinsp;\u0026le;\u0026thinsp;0.05 (***), using version 5 of the GraphPad Prism.\u003c/p\u003e \u003c/div\u003e"},{"header":"Results and Discussion","content":"\u003cp\u003eFollowing extraction, the crude extract was adsorbed onto silica gel, and different solvent ratios (dichloromethane\u0026ndash;hexane) were used to elute the prepared column. The eluents were collected in test tubes (Table \u003cspan class=\"InternalRef\"\u003e1\u003c/span\u003e), which were subsequently bulked (EP1\u0026ndash;EP11) together on the basis of thin-layer chromatographic profiles (Table \u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003e). Figure \u003cspan class=\"InternalRef\"\u003e1\u003c/span\u003e show the scheme from the extraction of the powdered plant to the point where the eluents were obtained.\u0026nbsp;\u003c/p\u003e\u0026nbsp;\u003ctable id=\"Tab1\" border=\"1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003epartially purified crude extract of \u003cem\u003eEmilia praetermissa\u003c/em\u003e\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eSolvent mix\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eSolvent ratio\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eVolume (mL)\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eTest tubes\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHexane\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e100\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e600\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1\u0026ndash;40\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHexane-dichloromethane\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e90:10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e300\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e41\u0026ndash;67\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHexane-dichloromethane\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e80:20\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e300\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e68\u0026ndash;91\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHexane-dichloromethane\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e70:30\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e300\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e92\u0026ndash;118\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHexane-dichloromethane\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e60:40\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e300\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e119\u0026ndash;142\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHexane-dichloromethane\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e50:50\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e300\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e143\u0026ndash;169\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHexane-dichloromethane\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e40:60\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e300\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e170\u0026ndash;193\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHexane-dichloromethane\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e30:70\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e300\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e194\u0026ndash;219\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHexane-dichloromethane\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e20:80\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e300\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e220\u0026ndash;242\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHexane-dichloromethane\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e10:90\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e300\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e243\u0026ndash;269\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eDichloromethane\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e100\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e300\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e270\u0026ndash;292\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003ctable id=\"Tab2\" border=\"1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eCoding of eluents from test tubes\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eCoding\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eTest tube\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEP1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e41\u0026ndash;67\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEP2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e68\u0026ndash;80\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEP3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e81\u0026ndash;96\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEP4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e97\u0026ndash;111\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEP5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e112\u0026ndash;143\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEP6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e144\u0026ndash;157\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEP7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e158\u0026ndash;197\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEP8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e198\u0026ndash;224\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEP9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e225\u0026ndash;240\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEP10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e241\u0026ndash;268\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEP11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e269\u0026ndash;292\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003c/p\u003e\n\u003ch3\u003eSusceptibility test\u003c/h3\u003e\n\u003cp\u003eThe antibacterial potential of the eluents was evaluated against five bacterial species, \u003cem\u003eB. subtilis, E. coli, K. pneumoniae, S. aureus\u003c/em\u003e and \u003cem\u003eP. aeruginosa\u003c/em\u003e, via the agar well diffusion method previously described by Babaiwa and coworkers [\u003cspan class=\"CitationRef\"\u003e22\u003c/span\u003e]. Ciprofloxacin was utilized as the reference drug at 0.5 mg/mL. \u003cem\u003eE. coli\u003c/em\u003e, \u003cem\u003eB. subtilis\u003c/em\u003e and \u003cem\u003eS. aureus\u003c/em\u003e were susceptible to 10 mg/ml EP1-EP4, EP6-EP9 and EP11, respectively (Table \u003cspan class=\"InternalRef\"\u003e3\u003c/span\u003e). The zone of inhibition was 12\u0026thinsp;\u0026plusmn;\u0026thinsp;0.12 mm (EP4) for \u003cem\u003eB. subtilis\u003c/em\u003e, ranging from 12 mm to 14 mm (EP1-EP3, EP6-EP9 and EP11) for \u003cem\u003eE. coli\u003c/em\u003e and 14 mm to 18 mm (EP1-EP3, EP6-EP9 and EP11) for \u003cem\u003eS. aureus\u003c/em\u003e. The effects of the EP2, EP3, EP7 and EP10 strains tested against \u003cem\u003eE. coli\u003c/em\u003e were significant compared with those of ciprofloxacin, with a level of significance at p\u0026thinsp;\u0026le;\u0026thinsp;0.05. Similarly, EP2, EP6 and EP11 were significantly different at p\u0026thinsp;\u0026le;\u0026thinsp;0.05 compared with the standard drug with \u003cem\u003eS. aureus\u003c/em\u003e. Resistance of \u003cem\u003eB. subtilis\u003c/em\u003e, EP4, EP5 and EP10 to both \u003cem\u003eE. coli\u003c/em\u003e and \u003cem\u003eS. aureus was observed with the EP1-EP3 and EP5-EP11 eluents\u003c/em\u003e. While maintaining similar concentrations, resistance was observed against \u003cem\u003eP. aeruginosa and K. pneumoniae\u003c/em\u003e via EP1 - EP11. Eluents that are more susceptible than ciprofloxacin (0.5 mg/mL) are known, although our previous study revealed the susceptibility of the \u003cem\u003eE. praetermissa\u003c/em\u003e dichloromethane fraction to \u003cem\u003eP. aeruginosa\u003c/em\u003e [\u003cspan class=\"CitationRef\"\u003e20\u003c/span\u003e]. This study further purified the dichloromethane fraction into various eluents (EP1 to EP11), and the activities against \u003cem\u003eS. aureus\u003c/em\u003e, \u003cem\u003eB. subtilis\u003c/em\u003e and \u003cem\u003eE. coli\u003c/em\u003e were noted. Resistance may have been observed in gram-negative bacteria, but susceptibility to \u003cem\u003eE. coli\u003c/em\u003e indicated that these eluents may possess specific related antibacterial actions. The specific mechanism by which these genes confer resistance could involve efflux pump action, permeability barrier induction, mutational or recombination changes in target enzymes and alterations in targets with decreased affinity for drug compounds [\u003cspan class=\"CitationRef\"\u003e23\u003c/span\u003e]. Additionally, resistance could hypothetically be due to virulence [\u003cspan class=\"CitationRef\"\u003e24\u003c/span\u003e\u0026ndash;\u003cspan class=\"CitationRef\"\u003e25\u003c/span\u003e]. The susceptibility of \u003cem\u003eS. aureus, E. coli\u003c/em\u003e and \u003cem\u003eB. subtilis\u003c/em\u003e to these eluents could imply their use in the treatment of skin infection and diarrhea caused by these bacteria, justifying their ethno-medicinal usage of this plant.\u0026nbsp;\u003c/p\u003e\u0026nbsp;\u003ctable id=\"Tab3\" border=\"1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eAntibacterial activity\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eBacteria\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eEP1\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eEP2\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eEP3\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eEP4\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eEP5\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eEP6\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eEP7\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eEP8\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eEP9\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eEP10\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eEP11\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eCiprofloxacin\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eEscherichia coli\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e14\u0026thinsp;\u0026plusmn;\u0026thinsp;0.20\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e13\u0026thinsp;\u0026plusmn;\u0026thinsp;0.11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e12\u0026thinsp;\u0026plusmn;\u0026thinsp;0.11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e13\u0026thinsp;\u0026plusmn;\u0026thinsp;0.10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e14\u0026thinsp;\u0026plusmn;\u0026thinsp;0.10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e13\u0026thinsp;\u0026plusmn;\u0026thinsp;0.11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e13\u0026thinsp;\u0026plusmn;\u0026thinsp;0.12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e13\u0026thinsp;\u0026plusmn;\u0026thinsp;0.00\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e13\u0026thinsp;\u0026plusmn;\u0026thinsp;0.11\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eKlebsiella pneumoniae\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e22\u0026thinsp;\u0026plusmn;\u0026thinsp;0.20\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003ePseudomonas aeruginosa\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e30\u0026thinsp;\u0026plusmn;\u0026thinsp;0.14\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eStaphylococcus aureus\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e16\u0026thinsp;\u0026plusmn;\u0026thinsp;0.10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e18\u0026thinsp;\u0026plusmn;\u0026thinsp;0.11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e15\u0026thinsp;\u0026plusmn;\u0026thinsp;0.21\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e18\u0026thinsp;\u0026plusmn;\u0026thinsp;0.10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e18\u0026thinsp;\u0026plusmn;\u0026thinsp;0.10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e18\u0026thinsp;\u0026plusmn;\u0026thinsp;0.08\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e18\u0026thinsp;\u0026plusmn;\u0026thinsp;0.05\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e14\u0026thinsp;\u0026plusmn;\u0026thinsp;0.20\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e26\u0026thinsp;\u0026plusmn;\u0026thinsp;0.12\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eBacillus subtilis\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e12\u0026thinsp;\u0026plusmn;\u0026thinsp;0.12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNZ\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e30\u0026thinsp;\u0026plusmn;\u0026thinsp;0.22\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003c/p\u003e\n\u003cp\u003eKeywords: NZ\u0026thinsp;=\u0026thinsp;No zone of inhibition; zones of inhibition were measured in millimetres.\u003c/p\u003e\n\u003cdiv id=\"Sec11\" class=\"Section2\"\u003e\n \u003ch2\u003e\u003cimg src=\"data:image/png;base64,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\"\u003e\u003cbr\u003e\u003c/h2\u003e\n \u003ch2\u003eFTIR analysis\u003c/h2\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec13\" class=\"Section2\"\u003e\n \u003cp\u003eThe functional groups in the EP1 eluent were determined from the absorption spectrum from the FTIR data. The absorption spectrum is provided in Fig. \u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003e, while a summary of the absorption range and specific functional groups in EP1 are provided in Table \u003cspan class=\"InternalRef\"\u003e4\u003c/span\u003e. The main functional groups include the O‒H bond of the hydroxyl group and the N‒H medium bond of the secondary amine and amide. C-H and C-C stretches were observed for saturated alkanes and rings. The C\u0026thinsp;=\u0026thinsp;C bonds and C\u0026thinsp;\u003cspan type=\"Underline\" class=\"Underline\" name=\"Emphasis\"\u003e\u0026equiv;\u003c/span\u003e\u0026thinsp;C stretches are indicative of alkene and alkyne groups. The H-C\u0026thinsp;=\u0026thinsp;O stretch for the aldehydric group and overtones was observed at 1919.60 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e, although it was weak. Towards the end of the group frequency region, N-O symmetric stretching and C-O stretching were observed, representing nitrile and aldehydric, amide and ester groups, respectively.\u0026nbsp;\u003c/p\u003e\u0026nbsp;\u003ctable id=\"Tab4\" border=\"1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 4\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eFTIR spectra interpretation\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eS/N\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eWavenumber (Reference)\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eWavenumber (Test Sample)\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eFunctional group assignment\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eInference\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e3650\u0026thinsp;\u0026minus;\u0026thinsp;3200\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e3336.00\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eO-H, N-H\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eN-H medium, H-bonded\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e3000\u0026thinsp;\u0026minus;\u0026thinsp;2850\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e2974.40\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC-H\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC-H stretching bond\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e3000\u0026thinsp;\u0026minus;\u0026thinsp;2850\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e2929.70\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC-H\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC-H stretching bond\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e2830\u0026thinsp;\u0026minus;\u0026thinsp;2695\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e2832.80\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eH-C\u0026thinsp;=\u0026thinsp;O\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eH-C\u0026thinsp;=\u0026thinsp;O stretch\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e2260\u0026thinsp;\u0026minus;\u0026thinsp;2100\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e2124.60\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e-C\u0026thinsp;\u003cspan type=\"Underline\" class=\"Underline\" name=\"Emphasis\"\u003e\u0026equiv;\u003c/span\u003e\u0026thinsp;C-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u0026thinsp;\u003cspan type=\"Underline\" class=\"Underline\" name=\"Emphasis\"\u003e\u0026equiv;\u003c/span\u003e\u0026thinsp;C stretch\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e2000\u0026thinsp;\u0026minus;\u0026thinsp;1665\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1919.60\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eOvertones, weak from this range\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1680\u0026thinsp;\u0026minus;\u0026thinsp;1620\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1636.30\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u0026thinsp;=\u0026thinsp;C\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u0026thinsp;=\u0026thinsp;C bonds\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1500\u0026thinsp;\u0026minus;\u0026thinsp;1400\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1449.90\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC-C\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC-C stretch in ring\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1360\u0026thinsp;\u0026minus;\u0026thinsp;1290\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1326.90\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eN-O\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eN-O symmetric stretch\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1260\u0026thinsp;\u0026minus;\u0026thinsp;1050\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1088.40\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC-O\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC-O stretch\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1300\u0026thinsp;\u0026minus;\u0026thinsp;1000\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1028.40\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC-O\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC-O stretch\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1000\u0026thinsp;\u0026minus;\u0026thinsp;650\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e879.70\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e=C-H\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e=C-H bend\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n \u003cp\u003e\u003c/p\u003e\n \u003cp\u003eGC‒MS analysis\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec14\" class=\"Section2\"\u003e\n \u003cp\u003eThe chromatogram of EP1 revealed fourteen compounds (peaks) (Fig. \u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003e), with retention times ranging from 4.792 min to 19.944 min. The prominent compounds were observed at 9.319 min, 9.719 min, 13.662 min, 13.902 min, and 19.944 min, with a total percentage area of 82.70%, while the individual percentage areas were as follows: 15.74%, 7.90%, 5.52%, 46.92%, and 6.62%, respectively.\u003c/p\u003e\n \u003cp\u003eThese fourteen compounds are mainly triazole, straight-chain alkanes, epoxide, amide and phthalate (Table \u003cspan class=\"InternalRef\"\u003e3\u003c/span\u003e). The prominent compounds are 2-ethyl-2H-benzotriazole (4.51%), N-(3-Allyl-2-oxo-2,3-dihydro-1,3-b enzothiazol-6-yl) acetamide (5.52%), bis(2-ethylhexyl) phthalate (6.62%), 2,4-di-tert-butylphenol (7.90%), (4aS)-3,5,5,9-tetramethyl-1,2,5,6,7,8-hexahydro-4a,8-epoxybenzo[\u003cspan class=\"CitationRef\"\u003e7\u003c/span\u003e]annulene (15.74%) and di-sec-butyl phthalate (46.92%). These findings indicate a high level of phthalates in EP1 (53.54%). In a previous study, bis-(2-ethylhexyl) phthalate was shown to have antibacterial activity against \u003cem\u003eE. coli\u003c/em\u003e and \u003cem\u003eS. aureus\u003c/em\u003e [\u003cspan class=\"CitationRef\"\u003e27\u003c/span\u003e]. In contrast, di-sec-butyl phthalate, which is isolated from the whole plant of \u003cem\u003eBegonia malabarica\u003c/em\u003e (Begoniaceae), has shown antibacterial potential against \u003cem\u003eK. pneumoniae, P. aeruginosa\u003c/em\u003e and \u003cem\u003eE. coli\u003c/em\u003e at 100 mg/mL [\u003cspan class=\"CitationRef\"\u003e28\u003c/span\u003e]. 2,4-Di-tert-butylphenol purified from the Streptomyces species KCA1 from \u003cem\u003ePhyllanthus niruri\u003c/em\u003e inhibited \u003cem\u003eS. aureus\u003c/em\u003e and \u003cem\u003eE. coli\u003c/em\u003e at 0.78 \u0026micro;g/mL and 50 \u0026micro;g/mL [\u003cspan class=\"CitationRef\"\u003e29\u003c/span\u003e]. These findings contradict those of this study, especially with respect to the inhibition of \u003cem\u003eK. pneumonia\u003c/em\u003e and \u003cem\u003eP. aeruginosa\u003c/em\u003e.\u0026nbsp;\u003c/p\u003e\n \u003ctable id=\"Tab5\" border=\"1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 5\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eCompounds identified from EP1 from the retention time, percentage area and molecular weight\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eS/N\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eRetention Time (min)\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003ePercentage Area\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eBase peak\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eMolecular weight\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eCompound\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e4.792\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e4.51\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e91.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e147.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2-ethyl-2H-Benzotriazole\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e7.013\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1.09\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e57.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e268.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNonadecane\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e9.319\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e15.74\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e161.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e218.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(4aS)-3,5,5,9-Tetramethyl-1,2,5,6,7,8-hexahydro-4a,8-epoxybenzo[\u003cspan class=\"CitationRef\"\u003e7\u003c/span\u003e]annulene\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e9.427\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1.87\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e57.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e212.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003ePentadecane\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e9.719\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e7.90\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e191.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e206.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2,4-Di-tert-butylphenol\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e11.539\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1.77\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e57.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e280.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHeptacosane\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e11.951\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1.34\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e57.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e240.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHeptadecane\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e13.427\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1.85\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e57.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e280.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHeptacosane\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e13.662\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e5.52\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e231.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e248.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eN-(3-Allyl-2-oxo-2,3-dihydro-1,3-b enzothiazol-6-yl) acetamide\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e13.902\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e46.92\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e149.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e278.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eDi-sec-butyl phthalate\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e15.138\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e2.33\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e57.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e282.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEicosane\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e15.464\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1.23\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e57.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e282.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEicosane\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e13\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e17.026\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e1.31\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e57.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e296.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHeneicosane\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e14\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e19.944\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e6.62\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e149.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e390.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eBis(2-ethylhexyl) phthalate\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n \u003cp\u003e\u003c/p\u003e\n \u003cp\u003eSavelli and coworkers [\u003cspan class=\"CitationRef\"\u003e30\u003c/span\u003e] reported the use of derivatives of 2-ethyl-2H-benzotriazole as antimicrobial agents, whereas Nuvole \u003cem\u003eet al\u003c/em\u003e. [\u003cspan class=\"CitationRef\"\u003e31\u003c/span\u003e] reported the susceptibility of \u003cem\u003eE. coli\u003c/em\u003e to acidic derivatives of benzotriazole. This compound is made of a benzene ring fused to a triazole; this triazole ring possesses antimicrobial activity, which can be partially or completely lost following annulation of the triazole ring at any point [\u003cspan class=\"CitationRef\"\u003e32\u003c/span\u003e]. Pentadecane, heptadecane, eicosane, heneicosane and nonadecane, with a percentage area of 12.79%, are straight alkanes; some have previously been reported in \u003cem\u003eE. coccinea\u003c/em\u003e [\u003cspan class=\"CitationRef\"\u003e33\u003c/span\u003e] to have antimicrobial activity [\u003cspan class=\"CitationRef\"\u003e34\u003c/span\u003e]. Derivatives of nonadecane have been reported to have moderate antibacterial effects against \u003cem\u003eE. coli, S. aureus, P. aeruginosa\u003c/em\u003e and \u003cem\u003eB. subtilis\u003c/em\u003e [\u003cspan class=\"CitationRef\"\u003e35\u003c/span\u003e\u0026ndash;\u003cspan class=\"CitationRef\"\u003e36\u003c/span\u003e]. The presence of these compounds in the eluent EP1 may be responsible for the antibacterial activity observed and could be synergistic in action against these bacteria.\u003c/p\u003e\n\u003c/div\u003e"},{"header":"Conclusion","content":"\u003cp\u003eGradient eluents from hexane-dichloromethane of \u003cem\u003eE. praetermissa\u003c/em\u003e were shown to possess antibacterial activity against selected clinical isolates. Antibacterial activity was observed in the EP1 eluent because of the presence of long-chain alkanes, benzotriazole, phthalate and butylphenol derivatives. These findings validate the use of \u003cem\u003eE. praetermissa\u003c/em\u003e in the treatment of skin infection and diarrhea, since some of the bacteria involved in these conditions are inhibited.\u003c/p\u003e "},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eContribution of Authors\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eBeauty Ighomena was involved in data collection and editing of the manuscript, whereas Emmanuel Eimiomodebheki Odion conceptualized the study and wrote and edited the manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgement:\u0026nbsp;\u003c/strong\u003eThe authors wish to especially thank Mr. Wilfred Aisagbonbuomwan, a laboratory staff member of the Department of Pharmaceutical Microbiology, for assisting with the microbial tests.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConflict of\u0026nbsp;\u003c/strong\u003e\u003cstrong\u003einterest:\u003c/strong\u003e There was no conflict of interest before, during or after the study.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding:\u003c/strong\u003e The success of this work was due to their personal contributions. Funding was not received throughout the study duration.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eChung, K-F., Ku, S-M., Kono, Y \u0026amp; Peng, C-I. \u003cem\u003eEmilia praetermissa\u003c/em\u003e Milne-Redh. (Asteraceae) - A Misidentified Alien Species in Northern Taiwan. \u003cem\u003eTaiwania\u003c/em\u003e 54 (4), 385-390 (2009).\u003c/li\u003e\n\u003cli\u003eGraveson, R. Plants of Saint Lucia: A Pictorial Flora of Wild and Cultivated Vascular Plants. (2016).\u003c/li\u003e\n\u003cli\u003ePOWO. Plants of the World Online. In: Plants of the World Online, London, UK: Royal Botanic Gardens, Kew. (2020).\u003c/li\u003e\n\u003cli\u003eLisowski, S. Le genre Emilia (Cass) Cass. 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Elsevier. 407 (2010).\u003c/li\u003e\n\u003cli\u003eNaeim, H., El-Hawiet, A., Abdel Rahman, R.A, Hussein, A., El Demellawy, M.A. \u0026amp; Amira M.\u003cem\u003e.\u003c/em\u003e Antibacterial Activity of \u003cem\u003eCentaurea pumilio\u003c/em\u003e L. Root and Aerial Part Extracts Against Some Multidrug Resistant Bacteria. \u003cem\u003eBMC Complement Medicine and Therapies\u003c/em\u003e 20, 79 (2020). https://doi.org/10.1186/s12906-020-2876-y.\u003c/li\u003e\n\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Emilia praetermissa, Staphylococcus aureus, Escherichia coli, Bacillus subtilis, gradient elution","lastPublishedDoi":"10.21203/rs.3.rs-6568149/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6568149/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003ePurpose\u003c/h2\u003e \u003cp\u003eThe search for better antibacterial agents from plants has resulted in the identification of molecules with existing analogues. \u003cem\u003eEmilia praetermissa\u003c/em\u003e is an herbaceous plant that is traditionally used to treat bacterial skin infections and diarrhea. This study aimed to identify compounds in partially purified \u003cem\u003eE. praetermissa\u003c/em\u003e and investigate their antibacterial activity.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e \u003cp\u003eGradient elution chromatography was used to achieve partial purification of the compounds. Gas chromatography‒mass spectrometry (GCMS) and Fourier transform infrared spectroscopy were used to identify the phytochemicals from the eluent of interest (EP1). The agar well diffusion plate protocol enabled the assessment of the susceptibility profile of the eluents (EP1\u0026ndash;EP11) (10 mg/mL) against clinical isolates: \u003cem\u003eBacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa\u003c/em\u003e and \u003cem\u003eStaphylococcus aureus\u003c/em\u003e.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eEP1 revealed fourteen compounds: 4aS-3,4,5,5,9-tetramethyl-1,25,6,7,8-hexahydro-4a,8-epoxybenzo[7[annulene (15.74%), N-(3-allyl-2-oxo-2,3-dihydro-1,3-b enzothiazol-6-yl) acetamide (5.52%), pentadecane (1.87%), heptacosane (3.52%), heptadecane (1.34%), nonadecane (1.09%), eicosane (3.56%), heneicosane (1.31%), di-sec-butyl phthalate (46.92%), bis(2-ethylhexyl) phthalate (6.62%), 2,4-di-tert-butylphenol (7.90%) and 2-ethyl-2H-benzotriazole (4.51%). The functional groups identified include O-H, N-H, C-H, C-C and C\u0026thinsp;=\u0026thinsp;C, which combine with the data from the GC‒MS. Susceptibility testing revealed the inhibition of \u003cem\u003eStaphylococcus aureus\u003c/em\u003e, \u003cem\u003eBacillus subtilis\u003c/em\u003e and \u003cem\u003eEscherichia coli\u003c/em\u003e by eluents EP1\u0026ndash;EP11, excluding EP4, EP5 and EP10.\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e \u003cp\u003eThe results show that the identified compounds inhibit the pathogenic bacteria responsible for inducing skin infections and diarrhea, confirming their traditional use.\u003c/p\u003e","manuscriptTitle":"Spectroscopic Analysis and Antibacterial Activity of Partially Purified Dichloromethane Fraction of Emilia praetermissa Milne-Redhead (Asteraceae) Leaf","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-05-02 07:57:14","doi":"10.21203/rs.3.rs-6568149/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"55868273-eded-4dcd-92c3-fdcb0a407fa3","owner":[],"postedDate":"May 2nd, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":47931890,"name":"Medicinal Chemistry"}],"tags":[],"updatedAt":"2025-05-02T07:57:14+00:00","versionOfRecord":[],"versionCreatedAt":"2025-05-02 07:57:14","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-6568149","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6568149","identity":"rs-6568149","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}