In vitro evaluation of critical ultrafiltration fluxes and transmembrane pressure in a high flux dialyzer

In vitro evaluation of critical ultrafiltration fluxes and transmembrane pressure in a high flux dialyzer | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article In vitro evaluation of critical ultrafiltration fluxes and transmembrane pressure in a high flux dialyzer Siavash SOHAN GIR, Nathalie GAYRARD, Alain FICHEUX, Jonas LAGET, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6271588/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 05 Jul, 2025 Read the published version in Scientific Reports → Version 1 posted 12 You are reading this latest preprint version Abstract In patients treated with haemodiafiltration, high convection volumes are considered beneficial. However it leads to pressure instability and membrane fouling. We aimed to identify critical ultrafiltration fluxes based on different approaches including the maximal global ultrafiltration coefficient ( G K D−UF−max ), and to test the influence of ultrafiltration on system stability and membrane fouling. Experiments of cross-flow filtration of a protein-containing fluid (cow milk) were performed. The ultrafiltration rate (QUF) was sequentially modified using a peristaltic pump and transmembrane pressure TMP was recorded. G K D−UF and TMP stability over time were assessed. QUF critical values were estimated from the G K D−UF , critical flux, irreversible fouling and sustainable flux approaches. Membrane fouling was observed by microscopy. Proteins from the feed, ultrafiltrate and retained on membrane were assessed by protein assays and SDS-PAGE. The G K D−UF−max approach identified QUF critical values close to the irreversible fouling and sustainable flux. When QUF exceeded critical values, major increase in TMP over time was observed and more clogged dialyzer fibres were detected. Utilizing QUF below the G K D−UF−max critical value lead to stableTMP over time and fewer clogged fibres therefore G K D−UF−max is helpful to identify the critical ultrafiltration rate and can be used to optimize ultrafiltration flow that prevent membrane fouling. Health sciences/Nephrology/Renal replacement therapy/Haemodialysis Physical sciences/Physics/Fluid dynamics Haemodiafiltration Ultrafiltration Permeability Membrane fouling Critical flux Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryInfoFile.docx Cite Share Download PDF Status: Published Journal Publication published 05 Jul, 2025 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 21 Apr, 2025 Reviews received at journal 14 Apr, 2025 Reviews received at journal 13 Apr, 2025 Reviews received at journal 11 Apr, 2025 Reviewers agreed at journal 08 Apr, 2025 Reviewers agreed at journal 05 Apr, 2025 Reviewers agreed at journal 04 Apr, 2025 Reviewers agreed at journal 04 Apr, 2025 Reviewers invited by journal 02 Apr, 2025 Editor assigned by journal 02 Apr, 2025 Submission checks completed at journal 28 Mar, 2025 First submitted to journal 28 Mar, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6271588","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":445900443,"identity":"e4f84041-4c18-498e-ba0c-6656a19e07d5","order_by":0,"name":"Siavash SOHAN 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Ultrafiltration, Permeability, Membrane fouling, Critical flux","lastPublishedDoi":"10.21203/rs.3.rs-6271588/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6271588/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eIn patients treated with haemodiafiltration, high convection volumes are considered beneficial. However it leads to pressure instability and membrane fouling. We aimed to identify critical ultrafiltration fluxes based on different approaches including the maximal global ultrafiltration coefficient (\u003csub\u003eG\u003c/sub\u003eK\u003csub\u003eD−UF−max\u003c/sub\u003e), and to test the influence of ultrafiltration on system stability and membrane fouling. Experiments of cross-flow filtration of a protein-containing fluid (cow milk) were performed. The ultrafiltration rate (QUF) was sequentially modified using a peristaltic pump and transmembrane pressure TMP was recorded. \u003csub\u003eG\u003c/sub\u003eK\u003csub\u003eD−UF\u003c/sub\u003e and TMP stability over time were assessed. QUF critical values were estimated from the \u003csub\u003eG\u003c/sub\u003eK\u003csub\u003eD−UF\u003c/sub\u003e, critical flux, irreversible fouling and sustainable flux approaches. Membrane fouling was observed by microscopy. Proteins from the feed, ultrafiltrate and retained on membrane were assessed by protein assays and SDS-PAGE.\u003c/p\u003e\n\u003cp\u003eThe \u003csub\u003eG\u003c/sub\u003eK\u003csub\u003eD−UF−max\u003c/sub\u003e approach identified QUF critical values close to the irreversible fouling and sustainable flux. When QUF exceeded critical values, major increase in TMP over time was observed and more clogged dialyzer fibres were detected. 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