Abstract
Particularly in dry and semi-arid areas, soil salinity and phosphorus deficiency are major factors limiting agricultural productivity (Rhoades and Loveday, 1990). For possible application in saline soil management, the current work sought to separate and describe salt-tolerant phosphate-solubilizing bacteria (PSB) from rhizospheric soil samples. Soil samples were collected from saline-affected agricultural fields, and serial dilutions were plated on Pikovskaya’s agar to screen for PSB based on halo zone formation. Selected isolates were further evaluated for quantitative phosphate solubilization in liquid media and tested for salt tolerance on media supplemented with increasing concentrations of NaCl (0–10%). Several isolates demonstrated efficient phosphate solubilization and sustained growth at NaCl concentrations of up to 7%, indicating moderate to high salt tolerance. These strains hold promise as bioinoculants for improving phosphorus availability and promoting plant growth in saline environments. Further studies, including molecular identification and greenhouse trials, are recommended to assess their efficacy under field conditions.
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Isolation of salt-tolerant phosphate-solubilizing bacteria for potential use in saline soil management | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 12 June 2025 V1 Latest version Share on Isolation of salt-tolerant phosphate-solubilizing bacteria for potential use in saline soil management Author : Kulbhushan Pawar 0009-0001-4736-9062 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174971526.65551372/v1 Published Journal of Advances in Microbiology Research Version of record Peer review timeline 175 views 96 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Particularly in dry and semi-arid areas, soil salinity and phosphorus deficiency are major factors limiting agricultural productivity (Rhoades and Loveday, 1990). For possible application in saline soil management, the current work sought to separate and describe salt-tolerant phosphate-solubilizing bacteria (PSB) from rhizospheric soil samples. Soil samples were collected from saline-affected agricultural fields, and serial dilutions were plated on Pikovskaya’s agar to screen for PSB based on halo zone formation. Selected isolates were further evaluated for quantitative phosphate solubilization in liquid media and tested for salt tolerance on media supplemented with increasing concentrations of NaCl (0–10%). Several isolates demonstrated efficient phosphate solubilization and sustained growth at NaCl concentrations of up to 7%, indicating moderate to high salt tolerance. These strains hold promise as bioinoculants for improving phosphorus availability and promoting plant growth in saline environments. Further studies, including molecular identification and greenhouse trials, are recommended to assess their efficacy under field conditions. Supplementary Material File (main.pdf) Download 318.34 KB File (photoplate psb.pdf) Download 669.39 KB Information & Authors Information Version history V1 Version 1 12 June 2025 Peer review timeline Published Journal of Advances in Microbiology Research Version of Record 1 Jul 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords biofertilizer phosphate- saline soil phosphate-solubilizing bacteria pikovskaya’s agar salt tolerance soil salinity Authors Affiliations Kulbhushan Pawar 0009-0001-4736-9062 [email protected] R B Narayanrao Borawake College View all articles by this author Metrics & Citations Metrics Article Usage 175 views 96 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Kulbhushan Pawar. Isolation of salt-tolerant phosphate-solubilizing bacteria for potential use in saline soil management. Authorea . 12 June 2025. 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