Lutein Attenuates Isoproterenol-Induced Cardiac Hypertrophy in Rats

Lutein Attenuates Isoproterenol-Induced Cardiac Hypertrophy in Rats | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Lutein Attenuates Isoproterenol-Induced Cardiac Hypertrophy in Rats Vinícius Cisneiros de Oliveira Santos, Michael Ramon Lima Conceição, and 9 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3967687/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 7 You are reading this latest preprint version Abstract Purpose Lutein (LUT) is a carotenoid found in fruits, and green vegetables with potent antioxidant, anti-inflammatory and cardioprotective action. However, the mechanisms involved in the cardioprotection against cardiac hypertrophy (CH) remains unkown. Objectives Investigate the anti-hypertrophic action of LUT in rats using the isoproterenol-induced CH model. Methods For CH induction, isoproterenol (ISO; 4.5 mg/kg/day, 7 days, i.p) was administrated and animals were treated with LUT (20 mg/kg/day, 7 days) or apocynin (APO, 10 mg/kg/day, 7 days). To investigate the participation of the nitric oxide (NO) pathway in the mechanism of action of LUT, the animals were treated with L-NAME (20 mg/kg/day, 7 days), an inhibitor of NO synthase. Results LUT and APO animals showed attenuated morphometric, fibrosis and inflammatory enhancement compared to ISO group, in addition to reducing the infarct area and the mortality rate triggered by ISO. Serum levels of CPK-TOTAL, CPK-MB, LDH, AST and ALT were significantly reduced in animals treated with LUT when compared to the ISO group. LUT attenuated the electrocardiographic changes induced by ISO (increase of QRS and QTc and inversion of T wave) and prevented the reduction of left ventricular pressure and heart rate in the ISO group. ISO increased the production of reactive oxygen species (ROS) in the heart which was prevented by LUT. ISO increased the Bax protein expression, which was attenuated by LUT treatment. Also, L-NAME partially reversed the LUT-mediated cardioprotection. Conclusion The results show that LUT exerts a cardioprotective effect against CH in rats partially related to NO pathway. Lutein carotenoid cardiac hypertrophy antioxidant isoproterenol rat Full Text Additional Declarations No competing interests reported. Supplementary Files DataAvailabilitydeclaration.xlsx SuplementaryBlot.tif Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 27 Mar, 2024 Reviews received at journal 25 Mar, 2024 Reviewers agreed at journal 16 Mar, 2024 Reviewers invited by journal 14 Mar, 2024 Editor assigned by journal 13 Mar, 2024 Submission checks completed at journal 13 Mar, 2024 First submitted to journal 18 Feb, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3967687","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":279631482,"identity":"a03eb23f-8530-49e1-b808-a9f7d160799a","order_by":0,"name":"Vinícius Cisneiros de Oliveira Santos","email":"","orcid":"","institution":"Universidade Federal de Sergipe","correspondingAuthor":false,"prefix":"","firstName":"Vinícius","middleName":"Cisneiros de Oliveira","lastName":"Santos","suffix":""},{"id":279631483,"identity":"069e2422-9772-4f1a-a664-ebdb33f313fc","order_by":1,"name":"Michael Ramon Lima 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Rats\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"naunyn-schmiedebergs-archives-of-pharmacology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"nsap","sideBox":"Learn more about [Naunyn-Schmiedeberg's Archives of Pharmacology](https://www.springer.com/journal/210)","snPcode":"210","submissionUrl":"https://submission.nature.com/new-submission/210/3","title":"Naunyn-Schmiedeberg's Archives of Pharmacology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Lutein, carotenoid, cardiac hypertrophy, antioxidant, isoproterenol, rat","lastPublishedDoi":"10.21203/rs.3.rs-3967687/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3967687/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003ePurpose\u003c/h2\u003e \u003cp\u003eLutein (LUT) is a carotenoid found in fruits, and green vegetables with potent antioxidant, anti-inflammatory and cardioprotective action. However, the mechanisms involved in the cardioprotection against cardiac hypertrophy (CH) remains unkown.\u003c/p\u003e\u003ch2\u003eObjectives\u003c/h2\u003e \u003cp\u003eInvestigate the anti-hypertrophic action of LUT in rats using the isoproterenol-induced CH model.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e \u003cp\u003eFor CH induction, isoproterenol (ISO; 4.5 mg/kg/day, 7 days, i.p) was administrated and animals were treated with LUT (20 mg/kg/day, 7 days) or apocynin (APO, 10 mg/kg/day, 7 days). To investigate the participation of the nitric oxide (NO) pathway in the mechanism of action of LUT, the animals were treated with L-NAME (20 mg/kg/day, 7 days), an inhibitor of NO synthase.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eLUT and APO animals showed attenuated morphometric, fibrosis and inflammatory enhancement compared to ISO group, in addition to reducing the infarct area and the mortality rate triggered by ISO. Serum levels of CPK-TOTAL, CPK-MB, LDH, AST and ALT were significantly reduced in animals treated with LUT when compared to the ISO group. LUT attenuated the electrocardiographic changes induced by ISO (increase of QRS and QTc and inversion of T wave) and prevented the reduction of left ventricular pressure and heart rate in the ISO group. ISO increased the production of reactive oxygen species (ROS) in the heart which was prevented by LUT. ISO increased the Bax protein expression, which was attenuated by LUT treatment. Also, L-NAME partially reversed the LUT-mediated cardioprotection.\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e \u003cp\u003eThe results show that LUT exerts a cardioprotective effect against CH in rats partially related to NO pathway.\u003c/p\u003e","manuscriptTitle":"Lutein Attenuates Isoproterenol-Induced Cardiac Hypertrophy in Rats","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-03-15 22:48:51","doi":"10.21203/rs.3.rs-3967687/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2024-03-27T09:42:08+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-03-25T09:17:40+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"c3198d05-7756-4af6-bfb8-4d5f2b9f6c32","date":"2024-03-16T16:38:23+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-03-14T13:43:39+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-03-13T07:26:49+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-03-13T07:26:48+00:00","index":"","fulltext":""},{"type":"submitted","content":"Naunyn-Schmiedeberg's Archives of Pharmacology","date":"2024-02-18T18:09:51+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"naunyn-schmiedebergs-archives-of-pharmacology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"nsap","sideBox":"Learn more about [Naunyn-Schmiedeberg's Archives of Pharmacology](https://www.springer.com/journal/210)","snPcode":"210","submissionUrl":"https://submission.nature.com/new-submission/210/3","title":"Naunyn-Schmiedeberg's Archives of Pharmacology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"b55147a9-52d1-4953-8368-871b20221006","owner":[],"postedDate":"March 15th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[],"tags":[],"updatedAt":"2024-04-25T03:55:24+00:00","versionOfRecord":[],"versionCreatedAt":"2024-03-15 22:48:51","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-3967687","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3967687","identity":"rs-3967687","version":["v1"]},"buildId":"J0_U0BvcaRcwD8yVFaRlm","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}