PENGARUH DAN MEKANISME KERJA EKSTRAK ETANOL BUAH MERAH (PANDANUS CONOIDEUS LAM) TERHADAP APOPTOSIS SEL GRANULOSA OVARIUM MENCIT MODEL ENDOMETRIOSIS MELALUI ANALISIS EKSPRESI TNF, NF-kB, CASPASE3, HSP70 DAN SOD

AIM: Purpose of this study was to prove whether the administration of red fruit extract therapy can reduce the incidence of apoptosis in ovarian granulosa cells of mice endometriosis model. METHODS:This study used experimental research design, 27 mice of endometriosis model (BalB / c) was divided into 3 groups, each consist of 9 mice: normal mice group group (K-), endometriosis model group received placebo (K+) and group of endometriosis model of mice given red fruit extract (P). samples were taken from the peritoneum to evaluate Tumor Necrosis Factor alpha (TNF) and Nuclear factor kappa beta (NF-kB) and ovaries for caspase 3, Heat shock protein (HSP70) and Superoxide dismutase (SOD). RESULTS :Administration of red fruit extract decrease granulosa cell apoptosis. The index of ovaries granulosa cell apoptosis in mice of K+ group was significantly higher compared to P group (p <0.05). TNF and NF-kB of endometriosis mice given red fruit extract was significantly lower than in the group endometriosis mice that received placebo (p <0.05). The decrease of TNF do not have correlation with the decrease of NF-kB (B=0,001;p=0,684). Caspase3 in the positive control group increased significantly compared with the negative control group (p <0.05). The SOD expression in P group was significantly higher than the K+ group (p <0.05). HSP 70 levels in the K+ group were higher than in the P (p <0.05). CONCLUSION : Red fruit ethanol extract decrease apoptosis of granulosa cells due to endometriosis, through the mechanism of decreasing TNF and caspase3 expressions. There is increase of SOD, and decrease of NF-kB, TNF and HSP70 after Red fruit ethanol extract administration. From pathway analysis shows that the decrease of NF-kB expression is not correlate with decrease of TNF. And the increase of SOD expression also not correlate with decrease of granulosa cell apoptotic index . Administration of red fruit ethanol extract causing HSP70 expression decrease. The decrease of apoptosis is not correlate with HSP70 expression decrease.