The effects of platelet rich plasma on healing of seawater immersion burn in rabbit

The effects of platelet rich plasma on healing of seawater immersion burn in rabbit | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article The effects of platelet rich plasma on healing of seawater immersion burn in rabbit Gan Zhang, Xiaosong Chen, Henglong Hu, Liang Hu, Congcong Chen, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8213837/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Objiective To investigate the effects of platelet-rich plasma (PRP) on healing of seawater immersion burn wound in rabbits. Method Seawater immersion burn model in rabbits was achieved by heating copper tube and seawater. 48 New Zealand rabbits were randomly divided into two groups ( group A and group B). Group A implanted nothing, and group B implanted PRP. The healing of seawater immersion burn wound in rabbits was detected by microscopic observation, histology, and immunohistochemistry, ELISA, and qPCR. Result The wound healing rate in the group B was significantly increased compared with group A. Compared with the group A, the rate of CD31-positive cells in group B was increased, while the contents of TNF-α and IL-1β were significantly decreased. The mRNA levels of VEGF, and PDGF in group B were evidently increased. Conclusion PRP can promote seawater immersion burn wound healing in rabbits by increasing the positive cell rate of CD31, upregulating the expression of VEGF, and TGF-β1 protein in wound tissue, and decreasing the contents of TNFα and IL-1β. PRP Burn Seawater Immersion CD31 Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8213837","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":555161598,"identity":"34433e8f-3631-4dcb-936f-35c1d1809a72","order_by":0,"name":"Gan Zhang","email":"","orcid":"","institution":"The 901th Hospital of the Joint Logistic Support of the Chinese people's Liberation Army","correspondingAuthor":false,"prefix":"","firstName":"Gan","middleName":"","lastName":"Zhang","suffix":""},{"id":555161601,"identity":"22ddfb40-aaa2-499f-9355-b76ade8608c3","order_by":1,"name":"Xiaosong 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CD31","lastPublishedDoi":"10.21203/rs.3.rs-8213837/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8213837/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eObjiective\u003c/strong\u003e To investigate the effects of platelet-rich plasma (PRP) on healing of seawater immersion burn wound in rabbits.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethod\u003c/strong\u003eSeawater immersion burn model in rabbits was achieved by heating copper tube and seawater. 48 New Zealand rabbits were randomly divided into two groups ( group A and group B). Group A implanted nothing, and group B implanted PRP. The healing of seawater immersion burn wound in rabbits was detected by microscopic observation, histology, and immunohistochemistry, ELISA, and qPCR.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult\u003c/strong\u003e The wound healing rate in the group B was significantly increased compared with group A. Compared with the group A, the rate of CD31-positive cells in group B was increased, while the contents of TNF-α and IL-1β were significantly decreased. The mRNA levels of VEGF, and PDGF in group B were evidently increased.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusion \u003c/strong\u003ePRP can promote seawater immersion burn wound healing in rabbits by increasing the positive cell rate of CD31, upregulating the expression of VEGF, and TGF-β1 protein in wound tissue, and decreasing the contents of TNFα and IL-1β.\u003c/p\u003e","manuscriptTitle":"The effects of platelet rich plasma on healing of seawater immersion burn in rabbit","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-12-24 11:55:34","doi":"10.21203/rs.3.rs-8213837/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"7a5f339a-32c4-4fec-a187-174269662043","owner":[],"postedDate":"December 24th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2026-03-27T11:56:04+00:00","versionOfRecord":[],"versionCreatedAt":"2025-12-24 11:55:34","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8213837","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8213837","identity":"rs-8213837","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}