Suppression of CGRP and TRPV1 Expression in Dorsal Root Ganglia of Knee Osteoarthritis Rats by Huojing Decoction via TrkA/MKK3/6/p38 Pathway

Suppression of CGRP and TRPV1 Expression in Dorsal Root Ganglia of Knee Osteoarthritis Rats by Huojing Decoction via TrkA/MKK3/6/p38 Pathway | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Suppression of CGRP and TRPV1 Expression in Dorsal Root Ganglia of Knee Osteoarthritis Rats by Huojing Decoction via TrkA/MKK3/6/p38 Pathway Xinchao Jiang, Yinyin Guo, Mei Fang, Xin Wang, Biao Zhang, Yi Song, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4485622/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Knee osteoarthritis (KOA) is a chronic condition characterized by persistent pain that can lead to severe disability. In this study, we primarily investigated the analgesic effect of Huojing decoction on MIA-induced knee arthritis. Methods MIA was injected to induce KOA pain in rats. Huojing decoction was then administered once daily via intragastric administration for 14 days. Pain level was assessed by measuring the paw withdrawal threshold (PWT) and paw withdrawal latency (PWL). ELISA determined serum levels of TNF-α, IL-1β, and IL-6. The Dorsal Root Ganglia (DRG) of L4 and L5 were collected from rats. TRPV1 and CGRP were detected through immunohistochemistry and immunofluorescence. The protein expression of TrkA, MKK3/6, and p38 was assessed by Western blot, and q-PCR measured the mRNA expression of TNF-α, IL-1β, and IL-6. Results Mechanical allodynia and thermal hyperalgesia were observed in KOA rats. The expression levels of inflammatory cytokines IL-1β, IL-6, and TNF-α were significantly decreased after Huojing decoction administration of KOA rats. Furthermore, administration of Huojing decoction in rats significantly decreased the mRNA expression levels of IL-1β, IL-6, and TNF-α in DRG. TRPV1 and CGRP levels were reduced with treatment. Conversely, the protein expressions of TrkA, MKK3/6, and p38 in the DRG of rats were significantly decreased. Conclusion Our data suggested that Huojing decoction can alleviate inflammation in KOA pain rats. Additionally, it can inhibit the expression of TrKA, MKK3/6, and p38 signaling pathways, indicating its analgesic effect on KOA pain rats. Huojing decoction Analgesic effect Knee osteoarthritis Dorsal root ganglion TrkA/MKK3/6/p38 Pathway Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4485622","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":310672451,"identity":"f1b42e1c-5609-4f5f-9231-3d3844c32193","order_by":0,"name":"Xinchao 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Pathway","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Huojing decoction, Analgesic effect, Knee osteoarthritis, Dorsal root ganglion, TrkA/MKK3/6/p38 Pathway","lastPublishedDoi":"10.21203/rs.3.rs-4485622/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4485622/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eBackground\u003c/h2\u003e \u003cp\u003eKnee osteoarthritis (KOA) is a chronic condition characterized by persistent pain that can lead to severe disability. In this study, we primarily investigated the analgesic effect of Huojing decoction on MIA-induced knee arthritis.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e \u003cp\u003eMIA was injected to induce KOA pain in rats. Huojing decoction was then administered once daily via intragastric administration for 14 days. Pain level was assessed by measuring the paw withdrawal threshold (PWT) and paw withdrawal latency (PWL). ELISA determined serum levels of TNF-α, IL-1β, and IL-6. The Dorsal Root Ganglia (DRG) of L4 and L5 were collected from rats. TRPV1 and CGRP were detected through immunohistochemistry and immunofluorescence. The protein expression of TrkA, MKK3/6, and p38 was assessed by Western blot, and q-PCR measured the mRNA expression of TNF-α, IL-1β, and IL-6.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eMechanical allodynia and thermal hyperalgesia were observed in KOA rats. The expression levels of inflammatory cytokines IL-1β, IL-6, and TNF-α were significantly decreased after Huojing decoction administration of KOA rats. Furthermore, administration of Huojing decoction in rats significantly decreased the mRNA expression levels of IL-1β, IL-6, and TNF-α in DRG. TRPV1 and CGRP levels were reduced with treatment. Conversely, the protein expressions of TrkA, MKK3/6, and p38 in the DRG of rats were significantly decreased.\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e \u003cp\u003eOur data suggested that Huojing decoction can alleviate inflammation in KOA pain rats. Additionally, it can inhibit the expression of TrKA, MKK3/6, and p38 signaling pathways, indicating its analgesic effect on KOA pain rats.\u003c/p\u003e","manuscriptTitle":"Suppression of CGRP and TRPV1 Expression in Dorsal Root Ganglia of Knee Osteoarthritis Rats by Huojing Decoction via TrkA/MKK3/6/p38 Pathway","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-06-10 05:44:47","doi":"10.21203/rs.3.rs-4485622/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"6ed50aff-ffef-4359-a637-ea7c0ea87913","owner":[],"postedDate":"June 10th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-07-21T11:56:51+00:00","versionOfRecord":[],"versionCreatedAt":"2024-06-10 05:44:47","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4485622","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4485622","identity":"rs-4485622","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}