Fel d 1-expressing plant-derived bioparticle: A novel treatment for cat allergy

Fel d 1-expressing plant-derived bioparticle: A novel treatment for cat allergy | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Allergy This is a preprint and has not been peer reviewed. Data may be preliminary. 12 September 2025 V1 Latest version Share on Fel d 1-expressing plant-derived bioparticle: A novel treatment for cat allergy Authors : Janice Layhadi , Liliana Cifuentes Gutierrez , Sean T. Keane , William Fulton 0000-0002-2803-5008 , Nichell Samson , Lily Y.D. Wu , Paulina Filipaviciute , … Show All … , Prista Hikmawati , Oleksandra Fedina , Ana Jimenez-Gil , Stephen Durham 0000-0001-5264-6207 , Guy Scadding , Guy Tropper , Louis‐Philippe Vézina , Patrick Colin , Ronald van Ree , and Mohamed H Shamji [email protected] Show Fewer Authors Info & Affiliations https://doi.org/10.22541/au.175766654.41680675/v1 Published Allergy Version of record Peer review timeline 409 views 263 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Allergen immunotherapy (AIT) is the only disease-modifying therapeutic approach for cat allergy, though it requires at least three years of treatment and can potentially induce severe systemic reactions. Plant-derived bioparticles expressing Fel d 1 allergen (Fel d 1 e BP) have been developed as a novel therapeutic candidate for cat allergy. Cellular and scRNA-seq analyses reveal that Fel d 1 e BP induce Th1 and IL-10 + T cells. We also demonstrate that Fel d 1 e BP targets the induction of protective metallothionine genes, CCL18 + monocytes and naïve B cells that are interferon responsive. Moreover, Fel d 1 e BP demonstrated reduced capacity to elicit basophil activation and histamine release, indicating their hypoallergenic nature. Administration of titrated doses of Fel d 1 e BP through skin prick test revealed that they are well-tolerated with reduced mean wheal compared to native Fel d 1 in open-label Phase 0 study. Altogether, we demonstrate that Fel d 1 e BP is hypo-allergenic and demonstrate tolerogenic properties making them a novel candidate for use in AIT for cat allergy. Supplementary Material File (fel d 1 bp manuscript_20jan_v2.docx) Download 177.00 KB File (figure 1.pptx) Download 1.15 MB File (figure 2.pptx) Download 2.62 MB File (figure 3.pptx) Download 6.27 MB File (figure 4.pptx) Download 5.36 MB File (figure 5.pptx) Download 4.09 MB File (figure 6.pptx) Download 1.12 MB Information & Authors Information Version history V1 Version 1 12 September 2025 Peer review timeline Published Allergy Version of Record 19 Mar 2026 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection Allergy Keywords allergy treament asthma biomarkers immunotherapy and tolerance induction Authors Affiliations Janice Layhadi Imperial College London National Heart and Lung Institute View all articles by this author Liliana Cifuentes Gutierrez Imperial College London National Heart and Lung Institute View all articles by this author Sean T. Keane Imperial College London National Heart and Lung Institute View all articles by this author William Fulton 0000-0002-2803-5008 Imperial College London National Heart and Lung Institute View all articles by this author Nichell Samson Imperial College London National Heart and Lung Institute View all articles by this author Lily Y.D. Wu Imperial College London National Heart and Lung Institute View all articles by this author Paulina Filipaviciute Imperial College London National Heart and Lung Institute View all articles by this author Prista Hikmawati Imperial College London National Heart and Lung Institute View all articles by this author Oleksandra Fedina Imperial College London National Heart and Lung Institute View all articles by this author Ana Jimenez-Gil Imperial College London National Heart and Lung Institute View all articles by this author Stephen Durham 0000-0001-5264-6207 Imperial College London National Heart and Lung Institute View all articles by this author Guy Scadding Imperial College London National Heart and Lung Institute View all articles by this author Guy Tropper Angany Inc View all articles by this author Louis‐Philippe Vézina Angany Inc View all articles by this author Patrick Colin Angany Inc View all articles by this author Ronald van Ree Amsterdam UMC Location University of Amsterdam View all articles by this author Mohamed H Shamji [email protected] Imperial College London National Heart and Lung Institute View all articles by this author Metrics & Citations Metrics Article Usage 409 views 263 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Janice Layhadi, Liliana Cifuentes Gutierrez, Sean T. Keane, et al. Fel d 1-expressing plant-derived bioparticle: A novel treatment for cat allergy. Authorea . 12 September 2025. DOI: https://doi.org/10.22541/au.175766654.41680675/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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