Effects of β-catenin siRNA on fibrotic markers in endometrial and endometriotic stromal cells from patients with endometriosis.

Sachiko Matsuzaki (405488), Claude Darcha (405489)
2015 · doi:10.1371/journal.pone.0076808.g001 · W6923010688
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⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-07-15 ⓘ

This study investigated the effects of β-catenin siRNA on fibrotic marker mRNA expression in endometrial and endometriotic stromal cells, observing specific changes with or without TGF-β1 stimulation.

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The study investigated whether silencing β-catenin affects the expression of fibrotic markers in stromal cells derived from people with endometriosis, using siRNA against β-catenin versus control siRNA. Endometriotic and endometrial stromal cells (n=10 each) were assessed for TGF-β1–stimulated and unstimulated mRNA levels of αSMA, Col-I, CTGF, and FN, with results reported relative to GAPDH. β-catenin siRNA altered the mRNA expression of these fibrosis-related markers in both endometriotic and endometrial stromal cells under basal conditions and after TGF-β1 stimulation, indicating involvement of the Wnt/β-catenin pathway in fibrosis-associated gene regulation. A limitation stated in the provided text is that outcomes are based on mRNA expression rather than protein or functional measures. This paper is centrally about endometriosis — it directly tests β-catenin siRNA effects on fibrotic marker expression in endometriotic stromal cells.

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Abstract

Effects of β-catenin siRNA on the mRNA expression of αSMA, Col-I, CTGF, and FN in endometriotic (A) and endometrial (B) stromal cells with or without TGF-β1 stimulation.\nC: control siRNA-transfected cells; ß: ß-catenin siRNA-transfected cells.\n*: p<.05 versus control (C) cells without TGF-β1 stimulation.\n**: p<.05 versus control (C) cells with TGF-β1 stimulation.\nNumerical values are presented as the mean + SEM. Expression levels of αSMA, Col-I, CTGF, and FN mRNA are given relative to the expression level of the reference gene, GAPDH.\nEndometriotic stromal cells (n=10), endometrial stromal cells (n=10).
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Involvement of the Wnt/β-Catenin Signaling Pathway in the Cellular and Molecular Mechanisms of Fibrosis in Endometriosis Figure 1 Effects of β-catenin siRNA on fibrotic markers in endometrial and endometriotic stromal cells from patients with endometriosis. Effects of β-catenin siRNA on the mRNA expression of αSMA, Col-I, CTGF, and FN in endometriotic (A) and endometrial (B) stromal cells with or without TGF-β1 stimulation. C: control siRNA-transfected cells; ß: ß-catenin siRNA-transfected cells. *: p<.05 versus control (C) cells without TGF-β1 stimulation. **: p<.05 versus control (C) cells with TGF-β1 stimulation. Numerical values are presented as the mean + SEM. Expression levels of αSMA, Col-I, CTGF, and FN mRNA are given relative to the expression level of the reference gene, GAPDH. Endometriotic stromal cells (n=10), endometrial stromal cells (n=10).

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endometriosis

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